RGD Reference Report - Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease. - Rat Genome Database

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Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.

Authors: Kikuchi, M  Hatano, N  Yokota, S  Shimozawa, N  Imanaka, T  Taniguchi, H 
Citation: Kikuchi M, etal., J Biol Chem. 2004 Jan 2;279(1):421-8. Epub 2003 Oct 15.
RGD ID: 1580664
Pubmed: PMID:14561759   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M305623200   (Journal Full-text)

Subcellular proteomics, which includes isolation of subcellular components prior to a proteomic analysis, is advantageous not only in characterizing large macro-molecular complexes such as organelles but also in elucidating mechanisms of protein transport and organelle biosynthesis. Because of the high sensitivity achieved by the present proteomics technology, the purity of samples to be analyzed is important for the interpretation of the results obtained. In the present study, peroxisomes isolated from rat liver by usual cell fractionation were further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70. The isolated peroxisomes were analyzed by SDS-PAGE combined with liquid chromatography/mass spectrometry. Altogether 34 known peroxisomal proteins were identified in addition to several mitochondrial and microsomal proteins. Some of the latter may reside in the peroxisomes as well. Analysis of membrane fractions identified all known peroxins except for Pex7. Two new peroxisomal proteins of unknown function were of high abundance. One is a bi-functional protein consisting of an aminoglycoside phosphotransferase-domain and an acyl-CoA dehydrogenase domain. The other is a newly identified peroxisome-specific isoform of Lon protease, an ATP-dependent protease with chaperone-like activity. The peroxisomal localization of the protein was confirmed by immunological techniques. The peroxisome-type Lon protease, which is distinct from the mitochondrial isoform, may play an important role in the peroxisomal biogenesis.

Objects Annotated

Genes (Rattus norvegicus)
Abcd2  (ATP binding cassette subfamily D member 2)
Abcd3  (ATP binding cassette subfamily D member 3)
Acaa1a  (acetyl-CoA acyltransferase 1A)
Acad11  (acyl-CoA dehydrogenase family, member 11)
Acox2  (acyl-CoA oxidase 2)
Acox3  (acyl-CoA oxidase 3, pristanoyl)
Acsl1  (acyl-CoA synthetase long-chain family member 1)
Agps  (alkylglycerone phosphate synthase)
Agxt  (alanine--glyoxylate aminotransferase)
Aldh3a2  (aldehyde dehydrogenase 3 family, member A2)
Amacr  (alpha-methylacyl-CoA racemase)
Baat  (bile acid CoA:amino acid N-acyltransferase)
Cat  (catalase)
Cps1  (carbamoyl-phosphate synthase 1)
Cyb5a  (cytochrome b5 type A)
Cyb5r3  (cytochrome b5 reductase 3)
Decr2  (2,4-dienoyl-CoA reductase 2)
Ehhadh  (enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase)
Fis1  (fission, mitochondrial 1)
Gnpat  (glyceronephosphate O-acyltransferase)
Gstk1  (glutathione S-transferase kappa 1)
Hao2  (hydroxyacid oxidase 2)
Hsd17b4  (hydroxysteroid (17-beta) dehydrogenase 4)
Idh1  (isocitrate dehydrogenase (NADP(+)) 1)
Isoc1  (isochorismatase domain containing 1)
Lonp2  (lon peptidase 2, peroxisomal)
Mgst1  (microsomal glutathione S-transferase 1)
Pdia6  (protein disulfide isomerase family A, member 6)
Pecr  (peroxisomal trans-2-enoyl-CoA reductase)
Pex1  (peroxisomal biogenesis factor 1)
Pex10  (peroxisomal biogenesis factor 10)
Pex11a  (peroxisomal biogenesis factor 11 alpha)
Pex11b  (peroxisomal biogenesis factor 11 beta)
Pex12  (peroxisomal biogenesis factor 12)
Pex13  (peroxisomal biogenesis factor 13)
Pex14  (peroxisomal biogenesis factor 14)
Pex16  (peroxisomal biogenesis factor 16)
Pex2  (peroxisomal biogenesis factor 2)
Pex3  (peroxisomal biogenesis factor 3)
Pex5  (peroxisomal biogenesis factor 5)
Pex6  (peroxisomal biogenesis factor 6)
Phyh  (phytanoyl-CoA 2-hydroxylase)
Prdx5  (peroxiredoxin 5)
Pxmp2  (peroxisomal membrane protein 2)
Pxmp4  (peroxisomal membrane protein 4)
Rab2a  (RAB2A, member RAS oncogene family)
Scp2  (sterol carrier protein 2)
Slc27a2  (solute carrier family 27 member 2)
Ugt1a1  (UDP glucuronosyltransferase family 1 member A1)
Uox  (urate oxidase)
Vapa  (VAMP associated protein A)

Objects referenced in this article
Gene Lonp1 lon peptidase 1, mitochondrial Rattus norvegicus

Additional Information