Cyb5r3 (cytochrome b5 reductase 3) - Rat Genome Database

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Gene: Cyb5r3 (cytochrome b5 reductase 3) Rattus norvegicus
Symbol: Cyb5r3
Name: cytochrome b5 reductase 3
RGD ID: 2502
Description: Enables cytochrome-b5 reductase activity, acting on NAD(P)H and nucleotide binding activity. Predicted to be involved in nitric oxide biosynthetic process. Located in endoplasmic reticulum membrane and mitochondrial outer membrane. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3); PARTICIPATES IN amino sugar metabolic pathway; nucleotide sugar metabolic pathway; INTERACTS WITH 1-naphthyl isothiocyanate; 17alpha-ethynylestradiol; 2,3,7,8-tetrachlorodibenzodioxine.
Type: protein-coding
Previously known as: B5R; Dia1; Diaphorase (NADH) (cytochrome b-5 reductase); diaphorase 1; diaphorase-1; NADH-cytochrome b5 reductase 3; Nadhcb5; RNNADHCB5
RGD Orthologs
Green Monkey
Naked Mole-Rat
Alliance Genes
More Info more info ...
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.27114,306,686 - 114,324,247 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl7114,306,685 - 114,324,298 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx7116,061,829 - 116,079,400 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.07118,286,246 - 118,303,784 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.07118,255,692 - 118,273,230 (-)NCBIRnor_WKY
Rnor_6.07124,024,003 - 124,041,564 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7124,023,995 - 124,041,594 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.07124,006,942 - 124,024,503 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.47121,187,821 - 121,205,382 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
RGSC_v3.17121,222,050 - 121,239,612 (-)NCBI
Celera7110,620,903 - 110,638,468 (-)NCBICelera
Cytogenetic Map7q34NCBI
JBrowse: View Region in Genome Browser (JBrowse)

Gene-Chemical Interaction Annotations     Click to see Annotation Detail View
1,2-dichloroethane  (ISO)
1,2-dimethylhydrazine  (ISO)
1-naphthyl isothiocyanate  (EXP)
17alpha-ethynylestradiol  (EXP)
2,2',4,4',5,5'-hexachlorobiphenyl  (ISO)
2,2',4,4'-Tetrabromodiphenyl ether  (ISO)
2,3,7,8-tetrachlorodibenzodioxine  (EXP,ISO)
2,4,6-tribromophenol  (ISO)
2,4-dinitrotoluene  (EXP)
2,6-dinitrotoluene  (EXP)
2-nitrofluorene  (EXP)
3-chloropropane-1,2-diol  (EXP)
3-isobutyl-1-methyl-7H-xanthine  (ISO)
3H-1,2-dithiole-3-thione  (EXP)
4-(N-nitrosomethylamino)-1-(3-pyridyl)butan-1-one  (EXP)
7,12-dimethyltetraphene  (ISO)
acrylamide  (EXP,ISO)
aflatoxin B1  (ISO)
alpha-hexachlorocyclohexane  (EXP)
ammonium chloride  (EXP)
aristolochic acid A  (ISO)
Aroclor 1254  (ISO)
arsenous acid  (ISO)
benzamidoxime  (ISO)
benzo[a]pyrene  (ISO)
benzo[a]pyrene diol epoxide I  (ISO)
Benzo[k]fluoranthene  (ISO)
bezafibrate  (EXP)
bifenthrin  (ISO)
bis(2-ethylhexyl) phthalate  (ISO)
bisphenol A  (EXP,ISO)
bisphenol F  (ISO)
butan-1-ol  (ISO)
C60 fullerene  (EXP)
cannabidiol  (ISO)
carbon nanotube  (ISO)
CGP 52608  (ISO)
chlordecone  (ISO)
choline  (ISO)
ciglitazone  (ISO)
cisplatin  (EXP,ISO)
clofibrate  (EXP)
clofibric acid  (EXP)
copper atom  (ISO)
copper(0)  (ISO)
copper(II) sulfate  (ISO)
crocidolite asbestos  (ISO)
cyclosporin A  (ISO)
decabromodiphenyl ether  (ISO)
dexamethasone  (EXP,ISO)
diarsenic trioxide  (ISO)
dibutyl phthalate  (EXP)
epoxiconazole  (ISO)
erythromycin estolate  (EXP)
ethanol  (EXP)
fenthion  (ISO)
flavonoids  (EXP)
flutamide  (EXP)
folic acid  (ISO)
genistein  (ISO)
glafenine  (EXP)
hydralazine  (ISO)
hydrogen cyanide  (ISO)
hydroxylamine  (ISO)
indometacin  (EXP,ISO)
ivermectin  (ISO)
ketamine  (EXP)
kojic acid  (EXP)
L-methionine  (ISO)
methidathion  (ISO)
methylmercury chloride  (ISO)
N(4)-hydroxycytidine  (ISO)
N-nitrosodiethylamine  (EXP)
N-nitrosodimethylamine  (EXP)
NAD zwitterion  (ISO)
NAD(+)  (ISO)
ochratoxin A  (ISO)
paracetamol  (EXP)
perfluorooctanoic acid  (EXP,ISO)
phenobarbital  (EXP,ISO)
phlorizin  (ISO)
pirinixic acid  (EXP,ISO)
potassium cyanide  (ISO)
pregnenolone 16alpha-carbonitrile  (EXP)
quercetin  (EXP)
quinidine  (EXP)
resveratrol  (ISO)
ritonavir  (EXP)
rotenone  (EXP)
sodium arsenite  (ISO)
sodium dichromate  (EXP)
sodium fluoride  (ISO)
Soman  (EXP)
sulfamethoxazole hydroxylamine  (ISO)
sunitinib  (ISO)
tamoxifen  (ISO)
temozolomide  (ISO)
testosterone  (ISO)
tetrachloromethane  (EXP,ISO)
tetracycline  (EXP)
thimerosal  (ISO)
thioacetamide  (EXP)
titanium dioxide  (ISO)
Triptolide  (ISO)
tunicamycin  (ISO)
valproic acid  (ISO)
vinclozolin  (EXP)
zinc atom  (ISO)
zinc(0)  (ISO)
zingerone  (EXP)
zoledronic acid  (ISO)

Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process

Cellular Component

Molecular Function

Molecular Pathway Annotations     Click to see Annotation Detail View

References - curated
# Reference Title Reference Citation
1. A case of methemoglobinemia type II due to NADH-cytochrome b5 reductase deficiency: determination of the molecular basis. Aalfs CM, etal., Hum Mutat 2000;16(1):18-22.
2. High-level expression in Escherichia coli of the soluble, catalytic domain of rat hepatic cytochrome b5 reductase. Barber MJ and Quinn GB, Protein Expr Purif. 1996 Aug;8(1):41-7. doi: 10.1006/prep.1996.0072.
3. The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent. Bewley MC, etal., Biochemistry. 2001 Nov 13;40(45):13574-82. doi: 10.1021/bi0106336.
4. The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site. Bewley MC, etal., Biochemistry. 2003 Nov 18;42(45):13145-51.
5. Polymorphisms in the carcinogen detoxification genes CYB5A and CYB5R3 and breast cancer risk in African American women. Blanke KL, etal., Cancer Causes Control. 2014 Nov;25(11):1513-21. doi: 10.1007/s10552-014-0454-7. Epub 2014 Sep 16.
6. A role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes. Borgese N, etal., J Cell Biol. 1996 Dec;135(6 Pt 1):1501-13. doi: 10.1083/jcb.135.6.1501.
7. Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands. Davis CA, etal., Arch Biochem Biophys 2002 Apr 1;400(1):63-75.
8. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
9. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
10. KEGG: Kyoto Encyclopedia of Genes and Genomes KEGG
11. Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease. Kikuchi M, etal., J Biol Chem. 2004 Jan 2;279(1):421-8. Epub 2003 Oct 15.
12. Molecular basis of recessive congenital methemoglobinemia, types I and II: Exon skipping and three novel missense mutations in the NADH-cytochrome b5 reductase (diaphorase 1) gene. Kugler W, etal., Hum Mutat. 2001 Apr;17(4):348.
13. Molecular basis of two novel mutations found in type I methemoglobinemia. Lorenzo FR 5th, etal., Blood Cells Mol Dis. 2011 Apr 15;46(4):277-81. doi: 10.1016/j.bcmd.2011.01.005. Epub 2011 Feb 24.
14. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Lund RR, etal., Mol Cell Proteomics. 2015 Nov;14(11):2988-99. doi: 10.1074/mcp.M115.050385. Epub 2015 Sep 8.
15. Rat ISS GO annotations from MGI mouse gene data--August 2006 MGD data from the GO Consortium
16. Heterogeneity of the rat NADH-cytochrome-b5-reductase transcripts resulting from multiple alternative first exons. Mota Vieira L, etal., Eur J Biochem 1994 Mar 15;220(3):729-37.
17. Electronic Transfer of LocusLink and RefSeq Data NCBI rat LocusLink and RefSeq merged data July 26, 2002
18. OMIM Disease Annotation Pipeline OMIM Disease Annotation Pipeline
19. A single mRNA, transcribed from an alternative, erythroid-specific, promoter, codes for two non-myristylated forms of NADH-cytochrome b5 reductase. Pietrini G, etal., J Cell Biol 1992 Jun;117(5):975-86.
20. Two transcripts encode rat cytochrome b5 reductase. Pietrini G, etal., Proc Natl Acad Sci U S A 1988 Oct;85(19):7246-50.
21. KEGG Annotation Import Pipeline Pipeline to import KEGG annotations from KEGG into RGD
22. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
23. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
24. Comprehensive gene review and curation RGD comprehensive gene curation
25. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, etal., Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. Epub 2002 Dec 11.
26. Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Zenno S, etal., J Biochem (Tokyo) 1990 Jun;107(6):810-6.
Additional References at PubMed
PMID:2498303   PMID:12865426   PMID:14741744   PMID:15489334   PMID:19946888   PMID:20833797   PMID:20861021   PMID:23376485   PMID:24945955   PMID:25850901   PMID:29706024  


Comparative Map Data
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.27114,306,686 - 114,324,247 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl7114,306,685 - 114,324,298 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx7116,061,829 - 116,079,400 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.07118,286,246 - 118,303,784 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.07118,255,692 - 118,273,230 (-)NCBIRnor_WKY
Rnor_6.07124,024,003 - 124,041,564 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7124,023,995 - 124,041,594 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.07124,006,942 - 124,024,503 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.47121,187,821 - 121,205,382 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
RGSC_v3.17121,222,050 - 121,239,612 (-)NCBI
Celera7110,620,903 - 110,638,468 (-)NCBICelera
Cytogenetic Map7q34NCBI
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
GRCh382242,617,840 - 42,649,392 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p13 Ensembl2242,615,730 - 42,720,870 (-)EnsemblGRCh38hg38GRCh38
GRCh372243,013,846 - 43,045,398 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 362241,344,763 - 41,375,349 (-)NCBINCBI36Build 36hg18NCBI36
Build 342241,339,318 - 41,367,516NCBI
Celera2226,924,326 - 26,955,882 (-)NCBICelera
Cytogenetic Map22q13.2NCBI
HuRef2225,974,473 - 26,005,949 (-)NCBIHuRef
CHM1_12242,972,995 - 43,004,519 (-)NCBICHM1_1
T2T-CHM13v2.02243,098,710 - 43,130,267 (-)NCBIT2T-CHM13v2.0
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
GRCm391583,037,696 - 83,060,641 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl1583,037,695 - 83,056,793 (-)EnsemblGRCm39 Ensembl
GRCm381583,153,495 - 83,176,440 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl1583,153,494 - 83,172,592 (-)EnsemblGRCm38mm10GRCm38
MGSCv371582,983,931 - 83,002,638 (-)NCBIGRCm37MGSCv37mm9NCBIm37
MGSCv361582,981,267 - 82,999,974 (-)NCBIMGSCv36mm8
Celera1585,286,802 - 85,305,568 (-)NCBICelera
Cytogenetic Map15E1NCBI
cM Map1539.4NCBI
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
ChiLan1.0 EnsemblNW_00495541327,969,949 - 27,979,909 (-)EnsemblChiLan1.0
ChiLan1.0NW_00495541327,971,140 - 27,979,905 (-)NCBIChiLan1.0ChiLan1.0
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
PanPan1.12241,666,548 - 41,694,104 (-)NCBIpanpan1.1PanPan1.1panPan2
PanPan1.1 Ensembl2241,666,548 - 41,694,104 (-)Ensemblpanpan1.1panPan2
Mhudiblu_PPA_v02223,513,724 - 23,544,746 (-)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
CanFam3.11022,829,898 - 22,848,284 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl1022,819,221 - 22,848,284 (+)EnsemblCanFam3.1canFam3CanFam3.1
Dog10K_Boxer_Tasha1022,756,034 - 22,783,841 (+)NCBIDog10K_Boxer_Tasha
ROS_Cfam_1.01023,565,239 - 23,592,996 (+)NCBIROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl1023,565,191 - 23,595,635 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.11023,282,809 - 23,310,756 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.01023,602,619 - 23,630,348 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.01023,777,463 - 23,805,206 (+)NCBIUU_Cfam_GSD_1.0
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
HiC_Itri_2NW_0244049456,411,149 - 6,427,796 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_004936718549,938 - 564,195 (-)EnsemblSpeTri2.0
SpeTri2.0NW_004936718552,369 - 564,195 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
Sscrofa11.1 Ensembl56,146,276 - 6,170,202 (+)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.156,146,230 - 6,170,209 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.253,604,124 - 3,628,138 (+)NCBISscrofa10.2Sscrofa10.2susScr3
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
ChlSab1.11925,172,513 - 25,191,139 (-)NCBIChlSab1.1ChlSab1.1chlSab2
Vero_WHO_p1.0NW_023666045100,266,413 - 100,298,273 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
(Heterocephalus glaber - naked mole-rat)
Naked Mole-rat AssemblyChrPosition (strand)SourceGenome Browsers
HetGla_female_1.0 EnsemblNW_0046247526,182,129 - 6,198,400 (+)EnsemblHetGla_female_1.0HetGla_female_1.0 EnsemblhetGla2
HetGla 1.0NW_0046247526,182,156 - 6,198,395 (+)NCBIHetGla_female_1.0HetGla 1.0hetGla2


Variants in Cyb5r3
63 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:648
Count of miRNA genes:243
Interacting mature miRNAs:292
Transcripts:ENSRNOT00000012878, ENSRNOT00000064700
Prediction methods:Microtar, Miranda, Rnahybrid
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (
For more information about miRGate, see PMID:25858286 or access the full paper here.

QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
634336Anxrr17Anxiety related response QTL 173.66locomotor behavior trait (VT:0001392)number of entries into a discrete space in an experimental apparatus (CMO:0000960)7924703115097879Rat
61357Bp38Blood pressure QTL 381.60.052arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)741333674119109060Rat
1300179Kidm5Kidney mass QTL 53.51kidney mass (VT:0002707)left kidney wet weight (CMO:0000083)743747012135012528Rat
631504Cm27Cardiac mass QTL 273.45heart left ventricle mass (VT:0007031)heart left ventricle wet weight (CMO:0000071)744421311118198041Rat
70173Niddm19Non-insulin dependent diabetes mellitus QTL 194.330.00005blood glucose amount (VT:0000188)blood glucose level area under curve (AUC) (CMO:0000350)764002457135012528Rat
631540Bw9Body weight QTL 94.5body mass (VT:0001259)body weight (CMO:0000012)769736226117455174Rat
634331Pia17Pristane induced arthritis QTL 174.7joint integrity trait (VT:0010548)arthritic paw count (CMO:0001460)773829340130221005Rat
7411654Foco25Food consumption QTL 259.30.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)775918751120918751Rat
7411607Foco15Food consumption QTL 150.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)775918751120918751Rat
1331728Bp214Blood pressure QTL 2142.825arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)780221299124373579Rat
1331768Kidm10Kidney mass QTL 104.62096kidney mass (VT:0002707)left kidney wet weight (CMO:0000083)780221299125221299Rat
2317052Aia17Adjuvant induced arthritis QTL 172.13joint integrity trait (VT:0010548)left rear ankle joint diameter (CMO:0002149)781737938126737938Rat
634322Bw12Body weight QTL 120body mass (VT:0001259)body weight (CMO:0000012)783153392128153392Rat
1358891Bp265Blood pressure QTL 2652.21arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)783591953134666232Rat
1358914Bp266Blood pressure QTL 266arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)783591953134666232Rat
71114Niddm14Non-insulin dependent diabetes mellitus QTL 144.5blood glucose amount (VT:0000188)plasma glucose level (CMO:0000042)784257275129257275Rat
2298475Eau6Experimental allergic uveoretinitis QTL 60.0029uvea integrity trait (VT:0010551)experimental autoimmune uveitis score (CMO:0001504)784257275129257275Rat
1558655Swd4Spike wave discharge measurement QTL 43.680.0002brain electrophysiology trait (VT:0010557)brain spike-and-wave discharge severity grade (CMO:0001988)786983365131983365Rat
1549899Stresp8Stress response QTL 84.370.0008stress-related behavior trait (VT:0010451)defensive burying duration (CMO:0001961)790482196135012528Rat
2299163Iddm34Insulin dependent diabetes mellitus QTL 342.71blood glucose amount (VT:0000188)age at onset/diagnosis of type 1 diabetes mellitus (CMO:0001140)791281130135012528Rat
631687Hcas1Hepatocarcinoma susceptibility QTL 13.90.001liver integrity trait (VT:0010547)liver tumorous lesion volume to total liver volume ratio (CMO:0001082)791412594129807172Rat
2313102Bmd79Bone mineral density QTL 792.30.0001tibia mineral mass (VT:1000283)total volumetric bone mineral density (CMO:0001728)794811085116738842Rat
1357336Gluco6Glucose level QTL 63.4blood glucose amount (VT:0000188)serum glucose level (CMO:0000543)794811326116294265Rat
731176Glom5Glomerulus QTL 52.50.0035kidney glomerulus morphology trait (VT:0005325)count of superficial glomeruli not directly contacting the kidney surface (CMO:0001002)796670164135012528Rat
1331731Bp216Blood pressure QTL 2162.851arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)7102297359133492884Rat
70159Bp61Blood pressure QTL 610.0001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)7103146217116738842Rat
731174Uae23Urinary albumin excretion QTL 232.40.0042urine albumin amount (VT:0002871)urine albumin excretion rate (CMO:0000757)7104603555135012528Rat
2306821Bp335Blood pressure QTL 3350.001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)7106571501135012528Rat
631663Bw6Body weight QTL 63.4body mass (VT:0001259)body weight (CMO:0000012)7111075573134976056Rat
1300112Bp183Blood pressure QTL 1833.51arterial blood pressure trait (VT:2000000)blood pressure time series experimental set point of the baroreceptor response (CMO:0002593)7111182207135012528Rat
1331748Bp215Blood pressure QTL 2154.043arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)7112308254133492884Rat
1357339Stl14Serum triglyceride level QTL 143.450.0001blood triglyceride amount (VT:0002644)serum triglyceride level (CMO:0000360)7112729683133492707Rat

Markers in Region
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.27114,321,734 - 114,321,932 (+)MAPPERmRatBN7.2
Rnor_6.07124,039,052 - 124,039,249NCBIRnor6.0
Rnor_5.07124,021,991 - 124,022,188UniSTSRnor5.0
RGSC_v3.47121,202,870 - 121,203,067UniSTSRGSC3.4
Celera7110,635,956 - 110,636,153UniSTS
Cytogenetic Map7q34UniSTS
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.27114,306,856 - 114,307,051 (+)MAPPERmRatBN7.2
Rnor_6.07124,024,174 - 124,024,368NCBIRnor6.0
Rnor_5.07124,007,113 - 124,007,307UniSTSRnor5.0
RGSC_v3.47121,187,992 - 121,188,186UniSTSRGSC3.4
Celera7110,621,074 - 110,621,268UniSTS
RH 3.4 Map7903.0UniSTS
Cytogenetic Map7q34UniSTS


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
Medium 3 43 57 41 19 41 8 11 74 35 39 11 8
Low 2
Below cutoff


Reference Sequences
RefSeq Acc Id: ENSRNOT00000064700   ⟹   ENSRNOP00000061381
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl7114,306,687 - 114,324,298 (-)Ensembl
Rnor_6.0 Ensembl7124,023,995 - 124,041,594 (-)Ensembl
RefSeq Acc Id: ENSRNOT00000102247   ⟹   ENSRNOP00000077900
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl7114,306,686 - 114,324,297 (-)Ensembl
RefSeq Acc Id: ENSRNOT00000111302   ⟹   ENSRNOP00000082175
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl7114,306,686 - 114,322,571 (-)Ensembl
RefSeq Acc Id: ENSRNOT00000118499   ⟹   ENSRNOP00000076621
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl7114,306,685 - 114,324,262 (-)Ensembl
RefSeq Acc Id: NM_138877   ⟹   NP_620232
Rat AssemblyChrPosition (strand)Source
mRatBN7.27114,306,686 - 114,324,247 (-)NCBI
Rnor_6.07124,024,003 - 124,041,564 (-)NCBI
Rnor_5.07124,006,942 - 124,024,503 (-)NCBI
RGSC_v3.47121,187,821 - 121,205,382 (-)RGD
Celera7110,620,903 - 110,638,468 (-)RGD
RefSeq Acc Id: XM_006242065   ⟹   XP_006242127
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.27114,306,686 - 114,322,577 (-)NCBI
Rnor_6.07124,024,003 - 124,039,895 (-)NCBI
Rnor_5.07124,006,942 - 124,024,503 (-)NCBI
RefSeq Acc Id: XM_039078438   ⟹   XP_038934366
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
mRatBN7.27114,306,695 - 114,322,267 (-)NCBI
Reference Sequences
RefSeq Acc Id: NP_620232   ⟸   NM_138877
- UniProtKB: Q64720 (UniProtKB/Swiss-Prot),   P20070 (UniProtKB/Swiss-Prot)
- Sequence:
RefSeq Acc Id: XP_006242127   ⟸   XM_006242065
- Peptide Label: isoform X1
- UniProtKB: P20070 (UniProtKB/Swiss-Prot)
- Sequence:
RefSeq Acc Id: ENSRNOP00000061381   ⟸   ENSRNOT00000064700
RefSeq Acc Id: XP_038934366   ⟸   XM_039078438
- Peptide Label: isoform X2
RefSeq Acc Id: ENSRNOP00000082175   ⟸   ENSRNOT00000111302
RefSeq Acc Id: ENSRNOP00000077900   ⟸   ENSRNOT00000102247
RefSeq Acc Id: ENSRNOP00000076621   ⟸   ENSRNOT00000118499
Protein Domains
FAD-binding FR-type

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-P20070-F1-model_v2 AlphaFold P20070 1-301 view protein structure


eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

RGD ID:13695544
Promoter ID:EPDNEW_R6069
Type:initiation region
Description:cytochrome b5 reductase 3
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database,
Experiment Methods:Single-end sequencing.
Rat AssemblyChrPosition (strand)Source
Rnor_6.07124,041,574 - 124,041,634EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:2502 AgrOrtholog
BioCyc Gene G2FUF-32607 BioCyc
Ensembl Genes ENSRNOG00000009592 Ensembl, ENTREZGENE, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
Ensembl Protein ENSRNOP00000061381.4 UniProtKB/TrEMBL
  ENSRNOP00000076621.1 UniProtKB/TrEMBL
  ENSRNOP00000077900 ENTREZGENE, UniProtKB/Swiss-Prot
  ENSRNOP00000082175 ENTREZGENE, UniProtKB/Swiss-Prot
Ensembl Transcript ENSRNOT00000064700.4 UniProtKB/TrEMBL
  ENSRNOT00000102247 ENTREZGENE, UniProtKB/Swiss-Prot
  ENSRNOT00000111302 ENTREZGENE, UniProtKB/Swiss-Prot
  ENSRNOT00000118499.1 UniProtKB/TrEMBL
Gene3D-CATH UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
InterPro CBR-like UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Cbr1-like_FAD-bd_dom UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  FAD-bd_FR_type UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Flavoprot_Pyr_Nucl_cyt_Rdtase UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  FNR_nucleotide-bd UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  OxRdtase_FAD/NAD-bd UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Riboflavin_synthase-like_b-brl UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
KEGG Report rno:25035 UniProtKB/Swiss-Prot
PANTHER PTHR19370 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
Pfam FAD_binding_6 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  NAD_binding_1 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
PhenoGen Cyb5r3 PhenoGen
Superfamily-SCOP SSF52343 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  SSF63380 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
UniProt A0A8I5Y5E9_RAT UniProtKB/TrEMBL
  NB5R3_RAT UniProtKB/Swiss-Prot, ENTREZGENE
UniProt Secondary Q64569 UniProtKB/Swiss-Prot
  Q64720 UniProtKB/Swiss-Prot

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2006-03-30 Cyb5r3  cytochrome b5 reductase 3  Dia1  diaphorase 1  Symbol and Name updated 1299863 APPROVED
2002-11-06 Dia1  diaphorase 1    Diaphorase (NADH) (cytochrome b-5 reductase)  Name updated 625702 APPROVED
2002-06-10 Dia1  Diaphorase (NADH) (cytochrome b-5 reductase)      Symbol and Name status set to approved 70586 APPROVED

RGD Curation Notes
Note Type Note Reference
gene_disease deficiency of the human homolog causes recessive congenital methemoglobinemia types I and II 728833