RGD Reference Report - Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. - Rat Genome Database

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Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.

Authors: Wilhelm, BG  Mandad, S  Truckenbrodt, S  Krohnert, K  Schafer, C  Rammner, B  Koo, SJ  Classen, GA  Krauss, M  Haucke, V  Urlaub, H  Rizzoli, SO 
Citation: Wilhelm BG, etal., Science. 2014 May 30;344(6187):1023-8. doi: 10.1126/science.1252884.
RGD ID: 10047219
Pubmed: PMID:24876496   (View Abstract at PubMed)
DOI: DOI:10.1126/science.1252884   (Journal Full-text)

Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.




Cellular Component
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Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Vamp1Ratpresynapse is_active_inEXP PMID:24876496SynGO 
Vamp1Ratpresynapse is_active_inIDA PMID:24876496SynGO 
Vamp1Ratpresynapse is_active_inIEP PMID:24876496SynGO 
ArhgdiaRatsynapse is_active_inEXP PMID:24876496SynGO 
ArhgdiaRatsynapse is_active_inIDA PMID:24876496SynGO 
Gdi2Ratsynapse is_active_inEXP PMID:24876496SynGO 
Gdi2Ratsynapse is_active_inIDA PMID:24876496SynGO 
Rab18Ratsynapse is_active_inEXP PMID:24876496SynGO 
Rab18Ratsynapse is_active_inIDA PMID:24876496SynGO 
Rab1aRatsynapse is_active_inEXP PMID:24876496SynGO 
Rab1aRatsynapse is_active_inIDA PMID:24876496SynGO 
Rab1bRatsynapse is_active_inEXP PMID:24876496SynGO 
Rab1bRatsynapse is_active_inIDA PMID:24876496SynGO 
Rab21Ratsynapse is_active_inEXP PMID:24876496SynGO 
Rab21Ratsynapse is_active_inIDA PMID:24876496SynGO 
Rab8aRatsynapse is_active_inEXP PMID:24876496SynGO 
Rab8aRatsynapse is_active_inIDA PMID:24876496SynGO 
AmphRatterminal bouton located_inHDA PMID:24876496ParkinsonsUK-UCL 
Ap2m1Ratterminal bouton located_inHDA PMID:24876496ParkinsonsUK-UCL 
AppRatterminal bouton located_inHDA PMID:24876496ParkinsonsUK-UCL 
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Genes (Rattus norvegicus)
Amph  (amphiphysin) Ap2m1  (adaptor related protein complex 2 subunit mu 1) App  (amyloid beta precursor protein)
Arhgdia  (Rho GDP dissociation inhibitor alpha) Atp6v0a1  (ATPase H+ transporting V0 subunit a1) Calb1  (calbindin 1)
Calb2  (calbindin 2) Cltc  (clathrin heavy chain) Cplx1  (complexin 1)
Cplx2  (complexin 2) Dnajc5  (DnaJ heat shock protein family (Hsp40) member C5) Epn1  (Epsin 1)
Gdi2  (GDP dissociation inhibitor 2) Hspa8  (heat shock protein family A (Hsp70) member 8) Itsn1  (intersectin 1)
Napa  (NSF attachment protein alpha) Pacsin1  (protein kinase C and casein kinase substrate in neurons 1) Pvalb  (parvalbumin)
Rab18  (RAB18, member RAS oncogene family) Rab1a  (RAB1A, member RAS oncogene family) Rab1b  (RAB1B, member RAS oncogene family)
Rab21  (RAB21, member RAS oncogene family) Rab5a  (RAB5A, member RAS oncogene family) Rab5al1  (Rab5a, member RAS oncogene family-like 1)
Rab7a  (RAB7A, member RAS oncogene family) Rab8a  (RAB8A, member RAS oncogene family) Scamp1  (secretory carrier membrane protein 1)
Septin5  (septin 5) Sgip1  (SH3GL interacting endocytic adaptor 1) Snap23  (synaptosome associated protein 23)
Snap25  (synaptosome associated protein 25) Snap29  (synaptosome associated protein 29) Snap91  (synaptosome associated protein 91)
Snca  (synuclein alpha) Sncb  (synuclein, beta) Stx6  (syntaxin 6)
Stx7  (syntaxin 7) Stxbp1  (syntaxin binding protein 1) Sv2a  (synaptic vesicle glycoprotein 2a)
Sv2b  (synaptic vesicle glycoprotein 2b) Syn1  (synapsin I) Syn2  (synapsin II)
Syngr1  (synaptogyrin 1) Syp  (synaptophysin) Syt1  (synaptotagmin 1)
Syt2  (synaptotagmin 2) Syt7  (synaptotagmin 7) Unc13a  (unc-13 homolog A)
Vamp1  (vesicle-associated membrane protein 1) Vamp2  (vesicle-associated membrane protein 2) Vti1a  (vesicle transport through interaction with t-SNAREs 1A)