RGD Reference Report - Regulatory T cell activity is partly inhibited in a mouse model of chronic Pseudomonas aeruginosa lung infection. - Rat Genome Database

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Regulatory T cell activity is partly inhibited in a mouse model of chronic Pseudomonas aeruginosa lung infection.

Authors: Ding, Feng-Ming  Zhu, Song-Lei  Shen, Ce  Ji, Xiao-Li  Zhou, Xin 
Citation: Ding FM, etal., Exp Lung Res. 2015 Feb;41(1):44-55. doi: 10.3109/01902148.2014.964351. Epub 2014 Nov 14.
RGD ID: 38501102
Pubmed: PMID:25398094   (View Abstract at PubMed)
DOI: DOI:10.3109/01902148.2014.964351   (Journal Full-text)


OBJECTIVES: We aimed to investigate the activity of regulatory T (Treg) cells in chronic Pseudomonas aeruginosa (PA) lung infection and its influence on effector T-cell responses.
MATERIALS AND METHODS: C57BL/6 mice were randomly inoculated with PA-laden agarose beads (1 × 10(5) CFU/50 μL) or planktonic PA (1 × 10(5) CFU/50 μL), and euthanized at the time points of 4 hour, day 1, 3, 5, and 7. Bacterial load, bronchoalveolar lavage (BAL) fluid cell counts, and lung tissue histology were assessed. BAL fluid concentrations of TGF-β1, IFN-γ, IL-1β, IL-4, IL-6, IL-10, and IL-17A were measured. Messenger RNA (mRNA) levels of TGF-β1, IL-10 and CD4(+) T-cell subtype-specific transcription factors were determined. The expression of CD4(+)CD25(+)forkhead box P3 (FoxP3)(+) cells in lungs and spleens were analyzed.
RESULTS: Mice inoculated with PA-laden agarose beads developed chronic PA lung infection during 7-day study period, while mice inoculated with planktonic PA cleared bacteria in 3 days. Compared with mice recovered from acute PA lung infection, those with chronic infection had significantly increased effector T-cell responses, accompanied by a more severe neutrophilic inflammation. Mice with chronic PA lung infection had significantly lower concentration of TGF-β1 and higher concentrations of IFN-γ, IL-1β, IL-6, and IL-17A in BAL fluid. Meanwhile, they had significantly lower mRNA levels of TGF-β1, IL-10 and FoxP3 in lung tissues, and lower expression of CD4(+)CD25(+)FoxP3(+) cells in lungs and spleens.
CONCLUSIONS: These findings indicate that Treg cell activity is partly inhibited in mice with chronic PA lung infection, which contributes to the enhanced effector T-cell responses in airways.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FOXP3Humanbacterial pneumonia severityISOFoxp3 (Mus musculus) RGD 
Foxp3Mousebacterial pneumonia severityIEP  RGD 
Foxp3Ratbacterial pneumonia severityISOFoxp3 (Mus musculus) RGD 
IFNGHumanbacterial pneumonia severityISOIfng (Mus musculus) RGD 
IL10Humanbacterial pneumonia severityISOIl10 (Mus musculus) RGD 
IL17AHumanbacterial pneumonia severityISOIl17a (Mus musculus) RGD 
IL1BHumanbacterial pneumonia severityISOIl1b (Mus musculus) RGD 
IL6Humanbacterial pneumonia severityISOIl6 (Mus musculus) RGD 
IfngRatbacterial pneumonia severityISOIfng (Mus musculus) RGD 
IfngMousebacterial pneumonia severityIEP  RGD 
Il10Ratbacterial pneumonia severityISOIl10 (Mus musculus) RGD 
Il10Mousebacterial pneumonia severityIEP  RGD 
Il17aRatbacterial pneumonia severityISOIl17a (Mus musculus) RGD 
Il17aMousebacterial pneumonia severityIEP  RGD 
Il1bRatbacterial pneumonia severityISOIl1b (Mus musculus) RGD 
Il1bMousebacterial pneumonia severityIEP  RGD 
Il6Ratbacterial pneumonia severityISOIl6 (Mus musculus) RGD 
Il6Mousebacterial pneumonia severityIEP  RGD 
RORCHumanbacterial pneumonia severityISORorc (Mus musculus) RGD 
RorcMousebacterial pneumonia severityIEP  RGD 
RorcRatbacterial pneumonia severityISORorc (Mus musculus) RGD 
TGFB1Humanbacterial pneumonia severityISOTgfb1 (Mus musculus) RGD 
Tgfb1Ratbacterial pneumonia severityISOTgfb1 (Mus musculus) RGD 
Tgfb1Mousebacterial pneumonia severityIEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Foxp3  (forkhead box P3)
Ifng  (interferon gamma)
Il10  (interleukin 10)
Il17a  (interleukin 17A)
Il1b  (interleukin 1 beta)
Il6  (interleukin 6)
Rorc  (RAR-related orphan receptor C)
Tgfb1  (transforming growth factor, beta 1)

Genes (Mus musculus)
Foxp3  (forkhead box P3)
Ifng  (interferon gamma)
Il10  (interleukin 10)
Il17a  (interleukin 17A)
Il1b  (interleukin 1 beta)
Il6  (interleukin 6)
Rorc  (RAR-related orphan receptor gamma)
Tgfb1  (transforming growth factor, beta 1)

Genes (Homo sapiens)
FOXP3  (forkhead box P3)
IFNG  (interferon gamma)
IL10  (interleukin 10)
IL17A  (interleukin 17A)
IL1B  (interleukin 1 beta)
IL6  (interleukin 6)
RORC  (RAR related orphan receptor C)
TGFB1  (transforming growth factor beta 1)


Additional Information