RGD Reference Report - Correlation of measurable serum markers of inflammation with lung levels following bilateral femur fracture in a rat model. - Rat Genome Database

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Correlation of measurable serum markers of inflammation with lung levels following bilateral femur fracture in a rat model.

Authors: Sears, BW  Volkmer, D  Yong, S  Himes, RD  Lauing, K  Morgan, M  Stover, MD  Callaci, JJ 
Citation: Sears BW, etal., J Inflamm Res. 2010 Aug 1;2010(3):105-114.
RGD ID: 5131471
Pubmed: PMID:21442011   (View Abstract at PubMed)
PMCID: PMC3062967   (View Article at PubMed Central)
DOI: DOI:10.2147/JIR.S12853   (Journal Full-text)

INTRODUCTION: Evaluation of the systemic inflammatory status following major orthopedic trauma has become an important adjunct in basing post-injury clinical decisions. In the present study, we examined the correlation of serum and lung inflammatory marker levels following bilateral femur fracture. MATERIALS AND METHODS: 45 Sprague Dawley rats underwent sham operation or bilateral femoral intramedullary pinning and mid-diaphyseal closed fracture via blunt guillotine. Animals were euthanized at specific time points after injury. Serum and lung tissue were collected, and 24 inflammatory markers were analyzed by immunoassay. Lung histology was evaluated by a blinded pathologist. RESULTS: Bilateral femur fracture significantly increased serum markers of inflammation including interleukin (IL)-2, IL-6, IL-10, GM-CSF, KC/GRO, MCP-1, and WBC. Femur fracture significantly increased serum and lung levels of IL-1a and KC/GRO at 6 hours. Lung levels of IL-6 demonstrated a trend towards significance. Histologic changes in pulmonary tissue after fracture included pulmonary edema and bone elements including cellular hematopoietic cells, bone fragments and marrow emboli. DISCUSSION AND CONCLUSION: Our results indicate that bilateral femur fracture with fixation in rats results in increases in serum markers of inflammation. Among the inflammatory markers measured, rise in the serum KC/GRO (CINC-1), a homolog to human IL-8, correlated with elevated levels of lung KC/GRO. Ultimately, analysis of serum levels of KC/GRO (CINC-1), or human IL-8, may be a useful adjunct to guide clinical decisions regarding surgical timing.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CSF2HumanFemoral Fractures  ISOCsf2 (Rattus norvegicus)protein:increased expression:serumRGD 
CXCL1HumanFemoral Fractures  ISOCxcl1 (Rattus norvegicus)protein:increased expression:lung and serumRGD 
Csf2RatFemoral Fractures  IEP protein:increased expression:serumRGD 
Csf2MouseFemoral Fractures  ISOCsf2 (Rattus norvegicus)protein:increased expression:serumRGD 
Cxcl1RatFemoral Fractures  IEP protein:increased expression:lung and serumRGD 
Cxcl1MouseFemoral Fractures  ISOCxcl1 (Rattus norvegicus)protein:increased expression:lung and serumRGD 
IL10HumanFemoral Fractures  ISOIl10 (Rattus norvegicus)protein:increased expression:serumRGD 
IL1AHumanFemoral Fractures  ISOIl1a (Rattus norvegicus)protein:increased expression:lung and serumRGD 
IL2HumanFemoral Fractures  ISOIl2 (Rattus norvegicus)protein:increased expression:serumRGD 
IL6HumanFemoral Fractures  ISOIl6 (Rattus norvegicus)protein:increased expression:serumRGD 
Il10RatFemoral Fractures  IEP protein:increased expression:serumRGD 
Il10MouseFemoral Fractures  ISOIl10 (Rattus norvegicus)protein:increased expression:serumRGD 
Il1aRatFemoral Fractures  IEP protein:increased expression:lung and serumRGD 
Il1aMouseFemoral Fractures  ISOIl1a (Rattus norvegicus)protein:increased expression:lung and serumRGD 
Il2RatFemoral Fractures  IEP protein:increased expression:serumRGD 
Il2MouseFemoral Fractures  ISOIl2 (Rattus norvegicus)protein:increased expression:serumRGD 
Il6RatFemoral Fractures  IEP protein:increased expression:serumRGD 
Il6MouseFemoral Fractures  ISOIl6 (Rattus norvegicus)protein:increased expression:serumRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Csf2Ratextracellular space  IDA  RGD 
Cxcl1Ratextracellular space  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csf2  (colony stimulating factor 2)
Cxcl1  (C-X-C motif chemokine ligand 1)
Il10  (interleukin 10)
Il1a  (interleukin 1 alpha)
Il2  (interleukin 2)
Il6  (interleukin 6)

Genes (Mus musculus)
Csf2  (colony stimulating factor 2 (granulocyte-macrophage))
Cxcl1  (C-X-C motif chemokine ligand 1)
Il10  (interleukin 10)
Il1a  (interleukin 1 alpha)
Il2  (interleukin 2)
Il6  (interleukin 6)

Genes (Homo sapiens)
CSF2  (colony stimulating factor 2)
CXCL1  (C-X-C motif chemokine ligand 1)
IL10  (interleukin 10)
IL1A  (interleukin 1 alpha)
IL2  (interleukin 2)
IL6  (interleukin 6)


Additional Information