Nefh (neurofilament heavy chain) - Rat Genome Database

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Gene: Nefh (neurofilament heavy chain) Rattus norvegicus
Symbol: Nefh
Name: neurofilament heavy chain
RGD ID: 3159
Description: Enables protein-macromolecule adaptor activity and toxic substance binding activity. A structural constituent of cytoskeleton. Involved in several processes, including cellular response to estradiol stimulus; nervous system development; and ovarian follicle atresia. Located in several cellular components, including dendrite; intermediate filament; and perikaryon. Used to study congenital hypothyroidism. Biomarker of glaucoma; hypothyroidism; phenylketonuria; sciatic neuropathy; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2CC; amyotrophic lateral sclerosis; amyotrophic lateral sclerosis type 1; and monoclonal gammopathy of uncertain significance. Orthologous to human NEFH (neurofilament heavy chain); PARTICIPATES IN amyotrophic lateral sclerosis pathway; INTERACTS WITH (R)-adrenaline; (R,R,R)-alpha-tocopherol; 1,2-dichlorobenzene.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: 200 kDa neurofilament protein; neurofilament heavy; neurofilament heavy polypeptide; neurofilament triplet H protein; neurofilament, heavy polypeptide; NF-H; Nfh
RGD Orthologs
Green Monkey
Naked Mole-Rat
Alliance Genes
More Info more info ...
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.21479,830,362 - 79,840,347 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl1479,830,362 - 79,840,351 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx1484,232,750 - 84,242,741 (-)NCBIRnor_SHRUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.01485,472,824 - 85,482,815 (-)NCBIRnor_SHRSPUTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.01481,922,138 - 81,932,117 (-)NCBIRnor_WKYUTH_Rnor_WKY_Bbb_1.0
Rnor_6.01485,181,572 - 85,191,557 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl1485,181,572 - 85,191,557 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.01485,859,193 - 85,869,187 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.41485,594,789 - 85,604,774 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
RGSC_v3.11485,614,415 - 85,623,919 (-)NCBI
Celera1478,720,827 - 78,730,812 (-)NCBICelera
Cytogenetic Map14q21NCBI
JBrowse: View Region in Genome Browser (JBrowse)

Gene-Chemical Interaction Annotations     Click to see Annotation Detail View
(R)-adrenaline  (EXP)
(R,R,R)-alpha-tocopherol  (EXP)
1,2-dichlorobenzene  (EXP)
1-chloro-2,4-dinitrobenzene  (ISO)
17alpha-ethynylestradiol  (EXP)
17beta-estradiol  (ISO)
2,2',4,4',5,5'-hexachlorobiphenyl  (ISO)
2,2',4,4'-Tetrabromodiphenyl ether  (EXP)
2,2',5,5'-tetrachlorobiphenyl  (ISO)
2,3,7,8-tetrachlorodibenzodioxine  (EXP)
2,5-hexanedione  (EXP)
3,3',4,4',5-pentachlorobiphenyl  (ISO)
3,3',5,5'-tetrabromobisphenol A  (EXP)
3,4-methylenedioxymethamphetamine  (EXP)
3-methyladenine  (ISO)
5-aza-2'-deoxycytidine  (ISO)
6-propyl-2-thiouracil  (EXP)
9-cis-retinoic acid  (ISO)
acetonitrile  (EXP)
aldicarb  (ISO)
all-trans-retinoic acid  (ISO)
amiodarone  (EXP)
ammonium chloride  (EXP)
antirheumatic drug  (ISO)
aristolochic acid A  (ISO)
arsane  (ISO)
arsenic atom  (ISO)
atrazine  (ISO)
atropine  (EXP)
benzo[a]pyrene  (ISO)
benzyl benzoate  (EXP)
bis(2-ethylhexyl) phthalate  (EXP)
bisphenol A  (EXP,ISO)
Brodifacoum  (EXP)
butanal  (ISO)
Butylbenzyl phthalate  (EXP)
cadmium dichloride  (EXP)
caffeine  (EXP)
cannabidiol  (ISO)
carbamazepine  (EXP)
carbaryl  (ISO)
carbon disulfide  (EXP)
carbonyl sulfide  (EXP)
CGP 52608  (ISO)
chlordecone  (ISO)
chlorpromazine  (EXP)
chlorpyrifos  (EXP)
cisplatin  (ISO)
clofibrate  (ISO)
cocaine  (EXP)
copper atom  (ISO)
copper(0)  (ISO)
copper(II) sulfate  (ISO)
Cuprizon  (EXP)
cycloheximide  (EXP)
cyclosporin A  (EXP,ISO)
cyfluthrin  (ISO)
cypermethrin  (ISO)
daidzein 7-O-beta-D-glucoside  (EXP)
deguelin  (ISO)
diazepam  (EXP)
diazinon  (EXP,ISO)
dibutyl phthalate  (EXP)
dichlorvos  (EXP)
dieldrin  (EXP)
diethyl phthalate  (EXP)
diisobutyl phthalate  (EXP)
diisononyl phthalate  (EXP)
dimethyl sulfoxide  (ISO)
diquat  (EXP)
disulfiram  (ISO)
dorsomorphin  (ISO)
doxycycline  (EXP)
entinostat  (ISO)
ethanol  (EXP)
ethylene glycol  (EXP)
fenvalerate  (EXP)
fulvestrant  (EXP)
genistein  (EXP)
gentamycin  (EXP)
glufosinate  (EXP)
glycerol  (EXP)
graphite  (EXP)
hydroquinone  (ISO)
lead diacetate  (ISO)
lead(0)  (ISO)
lead(II) chloride  (EXP)
lithium sulfate  (EXP)
malathion  (EXP)
manganese(II) chloride  (ISO)
melatonin  (ISO)
mercury dibromide  (ISO)
methanol  (EXP)
methylmercury chloride  (EXP,ISO)
Mipafox  (ISO)
mitoxantrone  (ISO)
morphine  (EXP)
N,N-dimethylformamide  (EXP)
N-acetyl-L-cysteine  (EXP)
ochratoxin A  (EXP)
octanoic acid  (EXP)
octyl gallate  (EXP)
orphenadrine  (EXP)
ozone  (ISO)
paraoxon  (ISO)
paraquat  (EXP)
parathion  (EXP)
pentachlorophenol  (EXP)
phenobarbital  (EXP)
phenylmercury acetate  (ISO)
picoxystrobin  (ISO)
pirinixic acid  (ISO)
potassium citrate monohydrate  (EXP)
potassium sulfate  (EXP)
progesterone  (ISO)
propan-2-ol  (EXP)
propanal  (ISO)
propranolol  (EXP)
resveratrol  (ISO)
Riluzole  (EXP)
rotenone  (ISO)
sarin  (EXP)
SB 431542  (ISO)
scopolamine  (ISO)
silicon dioxide  (ISO)
sodium arsenite  (EXP,ISO)
sodium hydrogencarbonate  (EXP)
Soman  (EXP)
streptozocin  (EXP)
sunitinib  (ISO)
T-2 toxin  (ISO)
tamoxifen  (ISO)
tetracycline  (EXP)
thapsigargin  (ISO)
thimerosal  (ISO)
thioacetamide  (EXP)
titanium dioxide  (ISO)
tributylstannane  (EXP)
Tributyltin oxide  (ISO)
trichlorfon  (ISO)
trichostatin A  (ISO)
trimethyltin  (EXP)
triphenyl phosphate  (EXP)
triptonide  (ISO)
tris(2-chloroethyl) phosphate  (EXP)
tropan-3alpha-yl 3-hydroxy-2-phenylpropanoate  (EXP)
Tryptanthrine  (ISO)
tunicamycin  (ISO)
urea  (EXP)
valproic acid  (EXP,ISO)
vorinostat  (ISO)

Gene Ontology Annotations     Click to see Annotation Detail View

Cellular Component

Molecular Pathway Annotations     Click to see Annotation Detail View

References - curated
# Reference Title Reference Citation
1. Deletions of the heavy neurofilament subunit tail in amyotrophic lateral sclerosis. Al-Chalabi A, etal., Hum Mol Genet 1999 Feb;8(2):157-64.
2. Heterodimeric associations between neuronal intermediate filament proteins. Athlan ES and Mushynski WE, J Biol Chem. 1997 Dec 5;272(49):31073-8.
3. Involvement of microglia in early axoglial alterations of the optic nerve induced by experimental glaucoma. Bordone MP, etal., J Neurochem. 2017 Jul;142(2):323-337. doi: 10.1111/jnc.14070. Epub 2017 Jun 9.
4. Partial sequence of the rat heavy neurofilament polypeptide (NF-H). Identification of putative phosphorylation sites [published erratum appears in FEBS Lett 1989 Feb 27;244(2):509] Breen KC, etal., FEBS Lett 1988 Dec 5;241(1-2):213-8.
5. Effects of brain ischemia on intermediate filaments of rat hippocampus. Camargo-De-Morais M, etal., Neurochem Res. 1996 May;21(5):595-602.
6. The C-terminal tail domain of neurofilament protein-H (NF-H) forms the crossbridges and regulates neurofilament bundle formation. Chen J, etal., J Cell Sci. 2000 Nov;113 Pt 21:3861-9.
7. Oxidative stress and 17-alpha- and 17-beta-estradiol modulate neurofilaments differently. Chiasson K, etal., J Mol Neurosci. 2006;30(3):297-310.
8. Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form. Chin SS and Liem RK, J Neurosci 1990 Nov;10(11):3714-26.
9. The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection. Dautigny A, etal., Biochem Biophys Res Commun 1988 Aug 15;154(3):1099-106.
10. Regioselective binding of 2,5-hexanedione to high-molecular-weight rat neurofilament proteins in vitro. DeCaprio AP, etal., Toxicol Appl Pharmacol. 1997 Jul;145(1):211-7.
11. The prognostic value of neurofilament levels in patients with sepsis-associated encephalopathy - A prospective, pilot observational study. Ehler J, etal., PLoS One. 2019 Jan 24;14(1):e0211184. doi: 10.1371/journal.pone.0211184. eCollection 2019.
12. Requirement of heavy neurofilament subunit in the development of axons with large calibers. Elder GA, etal., J Cell Biol 1998 Oct 5;143(1):195-205.
13. NF-M is an essential target for the myelin-directed "outside-in" signaling cascade that mediates radial axonal growth. Garcia ML, etal., J Cell Biol 2003 Dec 8;163(5):1011-20.
14. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
15. Perikaryal accumulation and proteolysis of neurofilament proteins in the post-mortem rat brain. Geddes JW, etal., Neurobiol Aging. 1995 Jul-Aug;16(4):651-60.
16. Regulation of neurofilament gene expression by thyroid hormone in the developing rat brain. Ghosh S, etal., Neuroreport. 1999 Aug 2;10(11):2361-5.
17. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
18. mRNA levels of all three neurofilament proteins decline following nerve transection. Goldstein ME, etal., Brain Res. 1988 Jun;427(3):287-91.
19. KEGG: Kyoto Encyclopedia of Genes and Genomes KEGG
20. Spontaneous perikaryal neurofilament phosphorylation in the septofimbrial nucleus of the rat. Klosen P and van den Bosch de Aguilar P, Neurosci Lett. 1992 May 11;139(1):108-13.
21. Overexpression of neurofilament subunit NF-L and NF-H extends survival of a mouse model for amyotrophic lateral sclerosis. Kong J and Xu Z, Neurosci Lett. 2000 Mar 3;281(1):72-4.
22. The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. Lees JF, etal., EMBO J 1988 Jul;7(7):1947-55.
23. Cerebrospinal fluid light and heavy neurofilament level increased in anti-N-methyl-d-aspartate receptor encephalitis. Li J, etal., Brain Behav. 2019 Aug;9(8):e01354. doi: 10.1002/brb3.1354. Epub 2019 Jul 17.
24. Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22. Lieberburg I, etal., Proc Natl Acad Sci U S A 1989 Apr;86(7):2463-7.
25. Vascular Endothelial Growth Factor-Transfected Bone Marrow Mesenchymal Stem Cells Improve the Recovery of Motor and Sensory Functions of Rats With Spinal Cord Injury. Liu X, etal., Spine (Phila Pa 1976). 2020 Apr 1;45(7):E364-E372. doi: 10.1097/BRS.0000000000003333.
26. Longitudinal Assessment of Transorbital Sonography, Visual Acuity, and Biomarkers for Inflammation and Axonal Injury in Optic Neuritis. Lochner P, etal., Dis Markers. 2017;2017:5434310. doi: 10.1155/2017/5434310. Epub 2017 Sep 11.
27. Differential expression and localization of the phosphorylated and nonphosphorylated neurofilaments during the early postnatal development of rat hippocampus. Lopez-Picon FR, etal., Hippocampus. 2003;13(7):767-79.
28. Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue. Mages B, etal., Front Cell Neurosci. 2018 Jun 18;12:161. doi: 10.3389/fncel.2018.00161. eCollection 2018.
29. Allopregnanolone prevents and suppresses oxaliplatin-evoked painful neuropathy: multi-parametric assessment and direct evidence. Meyer L, etal., Pain. 2011 Jan;152(1):170-81. doi: 10.1016/j.pain.2010.10.015. Epub 2010 Nov 10.
30. Rat ISS GO annotations from MGI mouse gene data--August 2006 MGD data from the GO Consortium
31. Overexpression of MAP2 and NF-H Associated with Dendritic Pathology in the Spinal Cord of Mice Infected with Rabies Virus. Monroy-Gómez J, etal., Viruses. 2018 Mar 6;10(3). pii: v10030112. doi: 10.3390/v10030112.
32. Chronic cocaine administration causes extensive white matter damage in brain: diffusion tensor imaging and immunohistochemistry studies. Narayana PA, etal., Psychiatry Res. 2014 Mar 30;221(3):220-30. doi: 10.1016/j.pscychresns.2014.01.005. Epub 2014 Jan 23.
33. Electronic Transfer of LocusLink and RefSeq Data NCBI rat LocusLink and RefSeq merged data July 26, 2002
34. OMIM Disease Annotation Pipeline OMIM Disease Annotation Pipeline
35. Neurofilament heavy chain expression and neuroplasticity in rat auditory cortex after unilateral and bilateral deafness. Park MH, etal., Hear Res. 2016 Sep;339:155-60. doi: 10.1016/j.heares.2016.07.010. Epub 2016 Jul 22.
36. Early in vitro genesis and differentiation of axons and dendrites by hippocampal neurons analyzed quantitatively with neurofilament-H and microtubule-associated protein 2 antibodies. Pennypacker K, etal., Exp Neurol. 1991 Jan;111(1):25-35.
37. The diagnostic and prognostic value of neurofilament heavy chain levels in immune-mediated optic neuropathies. Petzold A and Plant GT, Mult Scler Int. 2012;2012:217802. doi: 10.1155/2012/217802. Epub 2012 Dec 17.
38. Quantification of neurodegeneration by measurement of brain-specific proteins. Petzold A, etal., J Neuroimmunol. 2003 May;138(1-2):45-8. doi: 10.1016/s0165-5728(03)00092-4.
39. Axonal degeneration and inflammation in acute optic neuritis. Petzold A, etal., J Neurol Neurosurg Psychiatry. 2004 Aug;75(8):1178-80. doi: 10.1136/jnnp.2003.017236.
40. Treatment response in relation to inflammatory and axonal surrogate marker in multiple sclerosis. Petzold A, etal., Mult Scler. 2004 Jun;10(3):281-3. doi: 10.1191/1352458504ms1021sr.
41. The new global multiple sclerosis severity score (MSSS) correlates with axonal but not glial biomarkers. Petzold A, etal., Mult Scler. 2006 Jun;12(3):325-8. doi: 10.1191/135248505ms1277oa.
42. KEGG Annotation Import Pipeline Pipeline to import KEGG annotations from KEGG into RGD
43. Retarded development of neurons and oligodendroglia in rat forebrain produced by hyperphenylalaninemia results in permanent deficits in myelin despite long recovery periods. Reynolds R, etal., Exp Neurol. 1993 Dec;124(2):357-67.
44. GOA pipeline RGD automated data pipeline
45. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
46. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
47. Novel axonal distribution of neurofilament-H phosphorylated at the glycogen synthase kinase 3beta-phosphorylation site in its E-segment. Sasaki T, etal., J Neurosci Res. 2009 Nov 1;87(14):3088-97. doi: 10.1002/jnr.22148.
48. Neurotrophin-3 prevents the proximal accumulation of neurofilament proteins in sensory neurons of streptozocin-induced diabetic rats. Sayers NM, etal., Diabetes. 2003 Sep;52(9):2372-80.
49. Thyroid hormones stimulate expression and modification of cytoskeletal protein during rat sciatic nerve regeneration. Schenker M, etal., Brain Res. 2002 Dec 13;957(2):259-70.
50. Simultaneous up-regulation of neurofilament proteins during the postnatal development of the rat nervous system. Schlaepfer WW and Bruce J, J Neurosci Res. 1990 Jan;25(1):39-49.
51. [Tetramethylpyrazine accelerated spinal cord repair through regulation of caspase-3 and neurofilament protein expression: an acute spinal cord injury model in rats]. Shen ZX, etal., Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Aug;33(8):693-9.
52. Neurofilaments in blood and CSF for diagnosis and prediction of onset in Creutzfeldt-Jakob disease. Steinacker P, etal., Sci Rep. 2016 Dec 8;6:38737. doi: 10.1038/srep38737.
53. Anti-neurofilament antibodies in neuropathy with monoclonal gammopathy of undetermined significance produce experimental motor nerve conduction block. Stubbs EB Jr, etal., Acta Neuropathol. 2003 Feb;105(2):109-16. Epub 2002 Sep 13.
54. The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes. Takahashi N and Ishizuka B, Endocrinology. 2012 Apr;153(4):1990-8. doi: 10.1210/en.2011-2026. Epub 2012 Feb 7.
55. Tentative Sequence Identification Numbers Tentative Sequence Data IDs. TIGR Gene Index, Rat Data
56. Selective solubilization of high-molecular-mass neurofilament subunit during nerve regeneration. Tsuda M, etal., J Neurochem. 2000 Feb;74(2):860-8.
57. Arsenic-induced neurotoxicity in relation to toxicokinetics: effects on sciatic nerve proteins. Vahidnia A, etal., Chem Biol Interact. 2008 Nov 25;176(2-3):188-95. doi: 10.1016/j.cbi.2008.07.001. Epub 2008 Jul 12.
58. Artemin induced functional recovery and reinnervation after partial nerve injury. Wang R, etal., Pain. 2014 Mar;155(3):476-84. doi: 10.1016/j.pain.2013.11.007. Epub 2013 Nov 21.
59. DGKiota regulates presynaptic release during mGluR-dependent LTD. Yang J, etal., EMBO J. 2011 Jan 5;30(1):165-80. doi: 10.1038/emboj.2010.286. Epub 2010 Nov 30.
60. Acrylamide alters cytoskeletal protein level in rat sciatic nerves. Yu S, etal., Neurochem Res. 2006 Oct;31(10):1197-204. Epub 2006 Oct 17.
61. Congenital hypothyroidism is associated with intermediate filament misregulation, glutamate transporters down-regulation and MAPK activation in developing rat brain. Zamoner A, etal., Neurotoxicology. 2008 Nov;29(6):1092-9. doi: 10.1016/j.neuro.2008.09.004. Epub 2008 Sep 18.
62. Mechanisms Underlying H2O2-Evoked Carbonyl Modification of Cytoskeletal Protein and Axon Injury in PC-12 Cells. Zhang X, etal., Cell Physiol Biochem. 2018;48(3):1088-1098. doi: 10.1159/000491975. Epub 2018 Jul 24.
Additional References at PubMed
PMID:2878828   PMID:7536898   PMID:8110465   PMID:8634266   PMID:9313898   PMID:10221457   PMID:10461886   PMID:12130654   PMID:14561875   PMID:14651853   PMID:15248193   PMID:17114649  
PMID:17626162   PMID:17634366   PMID:18434031   PMID:18498707   PMID:18635547   PMID:18729720   PMID:20176735   PMID:20439489   PMID:20633607   PMID:20805831   PMID:21515322   PMID:21828286  
PMID:22763971   PMID:24327345   PMID:25452168   PMID:25869803   PMID:27040688   PMID:28259758   PMID:29476059   PMID:32357304  


Comparative Map Data
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.21479,830,362 - 79,840,347 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl1479,830,362 - 79,840,351 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx1484,232,750 - 84,242,741 (-)NCBIRnor_SHRUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.01485,472,824 - 85,482,815 (-)NCBIRnor_SHRSPUTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.01481,922,138 - 81,932,117 (-)NCBIRnor_WKYUTH_Rnor_WKY_Bbb_1.0
Rnor_6.01485,181,572 - 85,191,557 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl1485,181,572 - 85,191,557 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.01485,859,193 - 85,869,187 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.41485,594,789 - 85,604,774 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
RGSC_v3.11485,614,415 - 85,623,919 (-)NCBI
Celera1478,720,827 - 78,730,812 (-)NCBICelera
Cytogenetic Map14q21NCBI
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
GRCh382229,480,218 - 29,491,390 (+)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl2229,480,218 - 29,491,390 (+)EnsemblGRCh38hg38GRCh38
GRCh372229,876,207 - 29,887,379 (+)NCBIGRCh37GRCh37hg19GRCh37
Build 362228,206,219 - 28,217,279 (+)NCBINCBI36Build 36hg18NCBI36
Build 342228,200,772 - 28,211,829NCBI
Celera2213,676,024 - 13,687,118 (+)NCBICelera
Cytogenetic Map22q12.2NCBI
HuRef2212,839,995 - 12,851,105 (+)NCBIHuRef
CHM1_12229,835,584 - 29,846,662 (+)NCBICHM1_1
T2T-CHM13v2.02229,943,567 - 29,954,761 (+)NCBIT2T-CHM13v2.0
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
GRCm39114,888,754 - 4,898,064 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl114,888,754 - 4,898,064 (-)EnsemblGRCm39 Ensembl
GRCm38114,938,754 - 4,948,064 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl114,938,754 - 4,948,064 (-)EnsemblGRCm38mm10GRCm38
MGSCv37114,838,757 - 4,848,067 (-)NCBIGRCm37MGSCv37mm9NCBIm37
MGSCv36114,838,761 - 4,851,268 (-)NCBIMGSCv36mm8
Celera115,436,254 - 5,444,974 (-)NCBICelera
Cytogenetic Map11A1NCBI
cM Map113.12NCBI
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
ChiLan1.0 EnsemblNW_0049554553,735,410 - 3,744,107 (+)EnsemblChiLan1.0
ChiLan1.0NW_0049554553,735,330 - 3,744,714 (+)NCBIChiLan1.0ChiLan1.0
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
NHGRI_mPanPan12242,155,232 - 42,166,273 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v02210,525,738 - 10,536,788 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.12228,331,778 - 28,341,874 (+)NCBIpanpan1.1PanPan1.1panPan2
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
CanFam3.12622,726,523 - 22,735,129 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl2622,726,526 - 22,735,125 (+)EnsemblCanFam3.1canFam3CanFam3.1
Dog10K_Boxer_Tasha2622,598,106 - 22,606,671 (+)NCBIDog10K_Boxer_Tasha
ROS_Cfam_1.02623,100,617 - 23,109,200 (+)NCBIROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl2623,100,626 - 23,109,200 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.12622,810,029 - 22,818,553 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.02623,073,295 - 23,081,873 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.02623,138,184 - 23,146,781 (+)NCBIUU_Cfam_GSD_1.0
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
HiC_Itri_2NW_024405118112,417,611 - 112,427,289 (-)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_0049366573,443,485 - 3,453,133 (+)EnsemblSpeTri2.0SpeTri2.0 Ensembl
SpeTri2.0NW_0049366573,443,406 - 3,453,096 (+)NCBISpeTri2.0SpeTri2.0SpeTri2.0
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
Sscrofa11.1 Ensembl1446,559,860 - 46,568,088 (+)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.11446,559,698 - 46,568,686 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.21449,572,997 - 49,583,859 (+)NCBISscrofa10.2Sscrofa10.2susScr3
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
ChlSab1.11912,357,982 - 12,375,449 (+)NCBIChlSab1.1ChlSab1.1chlSab2
Vero_WHO_p1.0NW_023666045113,778,644 - 113,789,304 (-)NCBIVero_WHO_p1.0Vero_WHO_p1.0
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
HetGla_female_1.0 EnsemblNW_0046247475,806,645 - 5,815,554 (+)EnsemblHetGla_female_1.0HetGla_female_1.0 EnsemblhetGla2
HetGla 1.0NW_0046247475,806,576 - 5,816,158 (+)NCBIHetGla_female_1.0HetGla 1.0hetGla2


Variants in Nefh
43 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:72
Count of miRNA genes:60
Interacting mature miRNAs:68
Prediction methods:Miranda, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (
For more information about miRGate, see PMID:25858286 or access the full paper here.

QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
631839Niddm37Non-insulin dependent diabetes mellitus QTL 373.37blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)141103062295876975Rat
70187Pancm5Pancreatic morphology QTL 516.7pancreas mass (VT:0010144)pancreas weight to body weight ratio (CMO:0000630)143032009280829842Rat
2313048Bss84Bone structure and strength QTL 843.10.0001tibia strength trait (VT:1000284)tibia total energy absorbed before break (CMO:0001736)143766971982669719Rat
2313084Bss83Bone structure and strength QTL 832.90.0001tibia size trait (VT:0100001)tibia midshaft endosteal cross-sectional area (CMO:0001716)143766971982669719Rat
2313089Bss81Bone structure and strength QTL 813.40.0001body length (VT:0001256)body length, nose to rump (CMO:0000079)143766971982669719Rat
2313100Bss82Bone structure and strength QTL 8230.0001tibia size trait (VT:0100001)tibia midshaft cross-sectional area (CMO:0001717)143766971982669719Rat
738037Hcas6Hepatocarcinoma susceptibility QTL 62.93liver integrity trait (VT:0010547)liver nonremodeling tumorous lesion volume to total liver volume ratio (CMO:0001464)143905723783368335Rat
631523Pia13Pristane induced arthritis QTL 133.3joint integrity trait (VT:0010548)joint inflammation composite score (CMO:0000919)144079346098037301Rat
1300136Rf22Renal function QTL 223.9renal blood flow trait (VT:2000006)absolute change in renal vascular resistance (CMO:0001900)144226252995023211Rat
1549834Scl45Serum cholesterol level QTL 455.8blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)145002321195023211Rat
2300197Scl59Serum cholesterol level QTL 59blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)1455147478100147478Rat
9590294Uminl4Urine mineral level QTL 45.660.001urine mineral amount (VT:0015086)urine electrolyte level (CMO:0000593)1455624247100624247Rat
9589034Epfw11Epididymal fat weight QTL 1160.001epididymal fat pad mass (VT:0010421)epididymal fat pad weight to body weight ratio (CMO:0000658)1455624247100624247Rat
2317879Alcrsp27Alcohol response QTL 273.30.63response to alcohol trait (VT:0010489)duration of loss of righting reflex (CMO:0002289)1456631369101631369Rat
634328Hc5Hypercalciuria QTL 52.3urine calcium amount (VT:0002985)urine calcium excretion rate (CMO:0000763)1458184885103184885Rat
70153Bp59Blood pressure QTL 593.2arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)146875779683368335Rat
1582259Gluco23Glucose level QTL 233.10.0008blood glucose amount (VT:0000188)blood glucose level area under curve (AUC) (CMO:0000350)1470053989104886043Rat
1641900Alcrsp11Alcohol response QTL 11alcohol metabolism trait (VT:0015089)blood ethanol level (CMO:0000535)1470053989104886043Rat
1582197Gluco27Glucose level QTL 273.40.0006blood glucose amount (VT:0000188)blood glucose level area under curve (AUC) (CMO:0000350)147341532392554092Rat
1582209Gluco20Glucose level QTL 203.80.0005blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)147341532392554092Rat
1582236Gluco22Glucose level QTL 223.30.0164blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)147341532392554092Rat
1582255Gluco29Glucose level QTL 293.10.0025blood glucose amount (VT:0000188)absolute change in blood glucose level area under curve (CMO:0002034)147341532392554092Rat
1582250Gluco26Glucose level QTL 263.30.0009blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)147341532395876975Rat

Markers in Region
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21479,830,405 - 79,830,599 (+)MAPPERmRatBN7.2
Rnor_6.01485,181,616 - 85,181,809NCBIRnor6.0
Rnor_5.01485,859,237 - 85,859,430UniSTSRnor5.0
RGSC_v3.41485,594,833 - 85,595,026UniSTSRGSC3.4
Celera1478,720,871 - 78,721,064UniSTS
RH 3.4 Map14554.1UniSTS
Cytogenetic Map14q21UniSTS
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21479,830,370 - 79,830,547 (+)MAPPERmRatBN7.2
Rnor_6.01485,181,581 - 85,181,757NCBIRnor6.0
Rnor_5.01485,859,202 - 85,859,378UniSTSRnor5.0
RGSC_v3.41485,594,798 - 85,594,974UniSTSRGSC3.4
Celera1478,720,836 - 78,721,012UniSTS
Cytogenetic Map14q21UniSTS


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
Medium 4 38
Low 2 33 21 9 19 9 4 5 34 11 31 9 4
Below cutoff 1 9 12 12 12 4 5 2 24 10 2 4


RefSeq Acc Id: ENSRNOT00000011604   ⟹   ENSRNOP00000011604
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl1479,830,362 - 79,840,347 (-)Ensembl
Rnor_6.0 Ensembl1485,181,572 - 85,191,557 (-)Ensembl
RefSeq Acc Id: ENSRNOT00000110325   ⟹   ENSRNOP00000096290
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl1479,830,375 - 79,840,351 (-)Ensembl
RefSeq Acc Id: NM_012607   ⟹   NP_036739
RefSeq Status: VALIDATED
Rat AssemblyChrPosition (strand)Source
mRatBN7.21479,830,362 - 79,840,347 (-)NCBI
Rnor_6.01485,181,572 - 85,191,557 (-)NCBI
Rnor_5.01485,859,193 - 85,869,187 (-)NCBI
RGSC_v3.41485,594,789 - 85,604,774 (-)RGD
Celera1478,720,827 - 78,730,812 (-)RGD
RefSeq Acc Id: NP_036739   ⟸   NM_012607
- UniProtKB: F1LRZ7 (UniProtKB/TrEMBL)
- Sequence:
RefSeq Acc Id: ENSRNOP00000011604   ⟸   ENSRNOT00000011604
RefSeq Acc Id: ENSRNOP00000096290   ⟸   ENSRNOT00000110325
Protein Domains
IF rod

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-P16884-F1-model_v2 AlphaFold P16884 1-1072 view protein structure


eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

RGD ID:13699451
Promoter ID:EPDNEW_R9975
Type:single initiation site
Description:neurofilament heavy
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database,
Experiment Methods:Single-end sequencing.
Rat AssemblyChrPosition (strand)Source
Rnor_6.01485,191,571 - 85,191,631EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:3159 AgrOrtholog
BioCyc Gene G2FUF-15157 BioCyc
Ensembl Genes ENSRNOG00000008716 Ensembl, ENTREZGENE, UniProtKB/TrEMBL
Ensembl Transcript ENSRNOT00000011604 ENTREZGENE
  ENSRNOT00000011604.5 UniProtKB/TrEMBL
  ENSRNOT00000110325.1 UniProtKB/TrEMBL
Gene3D-CATH UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Single helix bin UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Vasodilator-stimulated phosphoprotein UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
InterPro DUF1388 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  IF_conserved UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  IF_rod_dom UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  NF-H UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  PTHR23214 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
Pfam DUF1388 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  Filament UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
PhenoGen Nefh PhenoGen
PROSITE IF_ROD_1 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
  IF_ROD_2 UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
RatGTEx ENSRNOG00000008716 RatGTEx
SMART Filament UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
Superfamily-SCOP Intermediate filament protein, coiled coil region UniProtKB/Swiss-Prot, UniProtKB/TrEMBL
TIGR TC204032
UniProt Secondary O35482 UniProtKB/Swiss-Prot
  Q540Z7 UniProtKB/Swiss-Prot
  Q63368 UniProtKB/Swiss-Prot

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2021-04-12 Nefh  neurofilament heavy chain  Nefh  neurofilament heavy  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2017-01-17 Nefh  neurofilament heavy  Nefh  neurofilament, heavy polypeptide  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2002-06-10 Nefh  Neurofilament, heavy polypeptide      Symbol and Name status set to approved 70586 APPROVED

RGD Curation Notes
Note Type Note Reference
gene_domains contains 51 Lys-Ser-Pro repeat triplets, which are possible sites of phosphorylation 728968