RGD Reference Report - Regulation of neurofilament gene expression by thyroid hormone in the developing rat brain. - Rat Genome Database

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Regulation of neurofilament gene expression by thyroid hormone in the developing rat brain.

Authors: Ghosh, S  Rahaman, SO  Sarkar, PK 
Citation: Ghosh S, etal., Neuroreport. 1999 Aug 2;10(11):2361-5.
RGD ID: 9743942
Pubmed: PMID:10439464   (View Abstract at PubMed)

The role of thyroid hormone (TH) in the expression of neurofilament (NF) genes during the first 3-4 postnatal weeks of rat brain development has been examined. I.p. administration of TH to 2-day-old hypothyroid rats resulted in a 2-fold increase in cerebral NF-M and NF-L mRNAs, while administration to 15-day-old hypothyroid rats led to a 1.5- to 3-fold increase in NF-H mRNA within 2-4 h of hormone injection. Comparison of the level of these mRNAs in cerebra from 5, 10, 15 and 20-day-old normal and hypothyroid rats by Northern blot analysis revealed that hypothyroidism declined the expression of all three mRNAs by 50-70% at all ages examined. Western blot analysis of total protein and cytoskeletal proteins isolated from cerebras of 5, 10, 15, 20 and 25-day-old normal and hypothyroid rats demonstrated an even greater reduction (60-90%) in the expression of NF proteins in the hypothyroid cerebra during the period examined. The overall results show that TH plays an important role in regulating the expression of all three NF genes during rat brain development.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypothyroidism treatmentISONefh (Rattus norvegicus)9743942; 9743942 RGD 
hypothyroidism treatmentISONefl (Rattus norvegicus)9743942; 9743942 RGD 
hypothyroidism treatmentISONefm (Rattus norvegicus)9743942; 9743942 RGD 
hypothyroidism treatmentIEP 9743942; 9743942; 9743942 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cerebral cortex development  IEP 9743942; 9743942; 9743942 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nefh  (neurofilament heavy chain)
Nefl  (neurofilament light chain)
Nefm  (neurofilament medium chain)

Genes (Mus musculus)
Nefh  (neurofilament, heavy polypeptide)
Nefl  (neurofilament, light polypeptide)
Nefm  (neurofilament, medium polypeptide)

Genes (Homo sapiens)
NEFH  (neurofilament heavy chain)
NEFL  (neurofilament light chain)
NEFM  (neurofilament medium chain)


Additional Information