RGD Reference Report - Selective solubilization of high-molecular-mass neurofilament subunit during nerve regeneration. - Rat Genome Database

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Selective solubilization of high-molecular-mass neurofilament subunit during nerve regeneration.

Authors: Tsuda, M  Tashiro, T  Komiya, Y 
Citation: Tsuda M, etal., J Neurochem. 2000 Feb;74(2):860-8.
RGD ID: 9698439
Pubmed: PMID:10646539   (View Abstract at PubMed)

A reduction in neurofilament (NF) protein synthesis and changes in their phosphorylation state are observed during nerve regeneration. To investigate how such metabolic changes are involved in the reorganization of the axonal cytoskeleton, we studied the injury-induced changes in the solubility and axonal transport of NF proteins as well as their phosphorylation states in the rat sciatic nerve. In the control nerve, 15-25% of high-molecular-mass NF subunit (NF-H) was recovered in the 1% Triton-soluble fraction when fractionated in the presence of phosphatase inhibitors. After a complete loss of NF proteins distal to the injury site (70-75 mm from the spinal cord) 1 week after injury, NF-H detected in the regenerating sprouts at 2 weeks or later exhibited higher solubility (>50%) and lower C-terminal phosphorylation level than NF-H in the control nerve. Solubility increase was also apparent with L-[35S]methionine-labeled NF-H that was in transit in the proximal axon at the time of injury. The low-molecular-mass subunit remained in the insoluble fraction in both the normal and the regenerating nerves, indicating that selective solubilization of NF-H rather than total filament disassembly occurs during regeneration.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NEFHHumanWallerian Degeneration  ISONefh (Rattus norvegicus)protein:decreased expression:neuronRGD 
NEFLHumanWallerian Degeneration  ISONefl (Rattus norvegicus)protein:decreased expression:neuronRGD 
NEFMHumanWallerian Degeneration  ISONefm (Rattus norvegicus)protein:decreased expression:neuronRGD 
NefhRatWallerian Degeneration  IEP protein:decreased expression:neuronRGD 
NefhMouseWallerian Degeneration  ISONefh (Rattus norvegicus)protein:decreased expression:neuronRGD 
NeflRatWallerian Degeneration  IEP protein:decreased expression:neuronRGD 
NeflMouseWallerian Degeneration  ISONefl (Rattus norvegicus)protein:decreased expression:neuronRGD 
NefmRatWallerian Degeneration  IEP protein:decreased expression:neuronRGD 
NefmMouseWallerian Degeneration  ISONefm (Rattus norvegicus)protein:decreased expression:neuronRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NefhRataxon regeneration  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nefh  (neurofilament heavy chain)
Nefl  (neurofilament light chain)
Nefm  (neurofilament medium chain)

Genes (Mus musculus)
Nefh  (neurofilament, heavy polypeptide)
Nefl  (neurofilament, light polypeptide)
Nefm  (neurofilament, medium polypeptide)

Genes (Homo sapiens)
NEFH  (neurofilament heavy chain)
NEFL  (neurofilament light chain)
NEFM  (neurofilament medium chain)


Additional Information