RGD Reference Report - Artemin induced functional recovery and reinnervation after partial nerve injury. - Rat Genome Database

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Artemin induced functional recovery and reinnervation after partial nerve injury.

Authors: Wang, Ruizhong  Rossomando, Anthony  Sah, Dinah W Y  Ossipov, Michael H  King, Tamara  Porreca, Frank 
Citation: Wang R, etal., Pain. 2014 Mar;155(3):476-84. doi: 10.1016/j.pain.2013.11.007. Epub 2013 Nov 21.
RGD ID: 27226886
Pubmed: PMID:24269493   (View Abstract at PubMed)
PMCID: PMC3936608   (View Article at PubMed Central)
DOI: DOI:10.1016/j.pain.2013.11.007   (Journal Full-text)

Systemic artemin promotes regeneration of dorsal roots to the spinal cord after crush injury. However, it is unclear whether systemic artemin can also promote peripheral nerve regeneration, and functional recovery after partial lesions distal to the dorsal root ganglion (DRG) remains unknown. In the present investigation, male Sprague Dawley rats received axotomy, ligation, or crush of the L5 spinal nerve or sham surgery. Starting the day of injury, animals received intermittent subcutaneous artemin or vehicle across 2weeks. Sensory thresholds to tactile or thermal stimuli were monitored for 6weeks after injury. Immunohistochemical analyses of the DRG and nerve regeneration were performed at the 6-week time point. Artemin transiently reversed tactile and thermal hypersensitivity after axotomy, ligation, or crush injury. Thermal and tactile hypersensitivity reemerged within 1week of treatment termination. However, artemin-treated rats with nerve crush, but not axotomy or ligation, subsequently showed gradual return of sensory thresholds to preinjury baseline levels by 6weeks after injury. Artemin normalized labeling for NF200, IB4, and CGRP in nerve fibers distal to the crush injury, suggesting persistent normalization of nerve crush-induced neurochemical changes. Sciatic and intradermal administration of dextran or cholera toxin B distal to the crush injury site resulted in labeling of neuronal profiles in the L5 DRG, suggesting regeneration functional restoration of nonmyelinated and myelinated fibers across the injury site into cutaneous tissue. Artemin also diminished ATF3 and caspase 3 expression in the L5 DRG, suggesting persistent neuroprotective actions. A limited period of artemin treatment elicits disease modification by promoting sensory reinnervation of distal territories and restoring preinjury sensory thresholds.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
NEFHHumanNerve Injuries treatmentISORGD:3159 RGD 
NefhRatNerve Injuries treatmentIEP  RGD 
NefhMouseNerve Injuries treatmentISORGD:3159 RGD 


Genes (Rattus norvegicus)
Nefh  (neurofilament heavy chain)

Genes (Mus musculus)
Nefh  (neurofilament, heavy polypeptide)

Genes (Homo sapiens)
NEFH  (neurofilament heavy chain)