RGD Reference Report - In vivo effect of surfactant on inflammatory cytokines during endotoxin-induced lung injury in rodents. - Rat Genome Database

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In vivo effect of surfactant on inflammatory cytokines during endotoxin-induced lung injury in rodents.

Authors: Mittal, N  Sanyal, SN 
Citation: Mittal N and Sanyal SN, J Immunotoxicol. 2011 Oct-Dec;8(4):274-83. Epub 2011 Jul 18.
RGD ID: 6480432
Pubmed: PMID:21767135   (View Abstract at PubMed)
DOI: DOI:10.3109/1547691X.2011.591294   (Journal Full-text)

Lipopolysaccharide (LPS) is a known inducer of acute respiratory distress syndrome (ARDS) in humans and animals. In this study, ARDS was developed in rats by intratracheal instillation of LPS and the effect of two types of surfactant (natural vs. synthetic) was examined to determine their potential corrective roles in general, as well as to compare the two surfactants against one another in particular, in endotoxin-induced lung injury. Sprague-Dawley male rats were divided into four groups, i.e., rats given: buffer controls; 055:B5 E. coli LPS only; LPS and then porcine surfactant (P-SF); or, LPS and then synthetic surfactant (S-SF). In vivo administration of LPS led to an increase in expression of the cytokines tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-2, IL-4, interferon-gamma, monocyte chemotactic protein-1, and macrophage inflammatory protein-1beta in the lungs of rats. These effects were confirmed by immunofluorescence in lung tissue sections and/or by protein (Western immunoblot) and mRNA expression (reverse transcription polymerase chain reaction) analyses of tissue samples. Apart from IL-4, concentrations of each of these cytokines in bronchoalveolar lavage fluid recovered from the animals were significantly increased in the LPS-treated hosts. Instillation of either surfactant (70 h after the LPS) into the airways diminished the expression of each of the inducible-cytokines, with the porcine (natural) form seeming having the greater inhibitory effect. These data suggest that surfactant can play an important role in the treatment of endotoxin-induced lung injury and might possess robust anti-inflammatory effects. Further, it seems that both the natural and synthetic surfactants prevent inflammatory outcomes in the lungs by controlling cytokine(s) production by various inflammatory cells. Last, the studies here clearly indicated that in this aspect, natural surfactant appears to be more beneficial compared to synthetic surfactant.



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Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
CCL2HumanAcute Lung Injury  ISOCcl2 (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
CCL4HumanAcute Lung Injury  ISOCcl4 (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
Ccl2RatAcute Lung Injury  IEP mRNA and protein:increased expression:lungRGD 
Ccl2MouseAcute Lung Injury  ISOCcl2 (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
Ccl4RatAcute Lung Injury  IEP mRNA and protein:increased expression:lungRGD 
Ccl4MouseAcute Lung Injury  ISOCcl4 (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
IFNGHumanAcute Lung Injury  ISOIfng (Rattus norvegicus) RGD 
IL1BHumanAcute Lung Injury  ISOIl1b (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
IL2HumanAcute Lung Injury  ISOIl2 (Rattus norvegicus)protein:increased expression:respiratory system fluid/secretionRGD 
IfngRatAcute Lung Injury  IEP  RGD 
IfngMouseAcute Lung Injury  ISOIfng (Rattus norvegicus) RGD 
Il1bRatAcute Lung Injury  IEP mRNA and protein:increased expression:lungRGD 
Il1bMouseAcute Lung Injury  ISOIl1b (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
Il2RatAcute Lung Injury  IEP protein:increased expression:respiratory system fluid/secretionRGD 
Il2MouseAcute Lung Injury  ISOIl2 (Rattus norvegicus)protein:increased expression:respiratory system fluid/secretionRGD 
TNFHumanAcute Lung Injury  ISOTnf (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
TnfRatAcute Lung Injury  IEP mRNA and protein:increased expression:lungRGD 
TnfMouseAcute Lung Injury  ISOTnf (Rattus norvegicus)mRNA and protein:increased expression:lungRGD 
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Genes (Rattus norvegicus)
Ccl2  (C-C motif chemokine ligand 2) Ccl4  (C-C motif chemokine ligand 4) Ifng  (interferon gamma)
Il1b  (interleukin 1 beta) Il2  (interleukin 2) Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Ccl2  (C-C motif chemokine ligand 2) Ccl4  (C-C motif chemokine ligand 4) Ifng  (interferon gamma)
Il1b  (interleukin 1 beta) Il2  (interleukin 2) Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
CCL2  (C-C motif chemokine ligand 2) CCL4  (C-C motif chemokine ligand 4) IFNG  (interferon gamma)
IL1B  (interleukin 1 beta) IL2  (interleukin 2) TNF  (tumor necrosis factor)

Gene CCL13 C-C motif chemokine ligand 13 Homo sapiens