RGD Reference Report - Chemokine-dependent neutrophil recruitment in a murine model of Legionella pneumonia: potential role of neutrophils as immunoregulatory cells. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Chemokine-dependent neutrophil recruitment in a murine model of Legionella pneumonia: potential role of neutrophils as immunoregulatory cells.

Authors: Tateda, K  Moore, TA  Newstead, MW  Tsai, WC  Zeng, X  Deng, JC  Chen, G  Reddy, R  Yamaguchi, K  Standiford, TJ 
Citation: Tateda K, etal., Infect Immun. 2001 Apr;69(4):2017-24.
RGD ID: 5135252
Pubmed: PMID:11254553   (View Abstract at PubMed)
PMCID: PMC98125   (View Article at PubMed Central)
DOI: DOI:10.1128/IAI.69.4.2017-2024.2001   (Journal Full-text)

The roles of CXC chemokine-mediated host responses were examined with an A/J mouse model of Legionella pneumophila pneumonia. After intratracheal inoculation of 10(6) CFU of L. pneumophila, the bacterial numbers in the lungs increased 10-fold by day 2; this increase was accompanied by the massive accumulation of neutrophils. Reverse transcription-PCR data demonstrated the up-regulation of CXC chemokines, such as keratinocyte-derived chemokine, macrophage inflammatory protein 2 (MIP-2), and lipopolysaccharide-induced CXC chemokine (LIX). Consistent with these data, increased levels of KC, MIP-2, and LIX proteins were observed in the lungs and peaked at days 1, 2, and 2, respectively. Although the administration of anti-KC or anti-MIP-2 antibody resulted in an approximately 20% decrease in neutrophil recruitment on day 2, no increase in mortality was observed. In contrast, the blockade of CXC chemokine receptor 2 (CXCR2), a receptor for CXC chemokines, including KC and MIP-2, strikingly enhanced mortality; this effect coincided with a 67% decrease in neutrophil recruitment. Interestingly, anti-CXCR2 antibody did not affect bacterial burden by day 2, even in the presence of a lethal challenge of bacteria. Moreover, a significant decrease in interleukin-12 (IL-12) levels, in contrast to the increases in KC, MIP-2, and LIX levels, was demonstrated for CXCR2-blocked mice. These data indicated that CXCR2-mediated neutrophil accumulation may play a crucial role in host defense against L. pneumophila pneumonia in mice. The increase in lethality without a change in early bacterial clearance suggested that neutrophils may exert their protective effect not through direct killing but through more immunomodulatory actions in L. pneumophila pneumonia. We speculate that a decrease in the levels of the protective cytokine IL-12 may explain, at least in part, the high mortality in the setting of reduced neutrophil recruitment.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CXCL1HumanLegionnaires' disease  ISOCxcl1 (Mus musculus)mRNA and protein:increased expression:lungRGD 
CXCL2HumanLegionnaires' disease  ISOCxcl2 (Mus musculus)mRNA and protein:increased expression:lungRGD 
CXCL5HumanLegionnaires' disease  ISOCxcl5 (Mus musculus)mRNA and protein:increased expression:lungRGD 
CXCR2HumanLegionnaires' disease  ISOCxcr2 (Mus musculus) RGD 
Cxcl1RatLegionnaires' disease  ISOCxcl1 (Mus musculus)mRNA and protein:increased expression:lungRGD 
Cxcl1MouseLegionnaires' disease  IEP mRNA and protein:increased expression:lungRGD 
Cxcl2RatLegionnaires' disease  ISOCxcl2 (Mus musculus)mRNA and protein:increased expression:lungRGD 
Cxcl2MouseLegionnaires' disease  IEP mRNA and protein:increased expression:lungRGD 
Cxcl5MouseLegionnaires' disease  IEP mRNA and protein:increased expression:lungRGD 
Cxcl6RatLegionnaires' disease  ISOCxcl5 (Mus musculus)mRNA and protein:increased expression:lungRGD 
Cxcr2RatLegionnaires' disease  ISOCxcr2 (Mus musculus) RGD 
Cxcr2MouseLegionnaires' disease  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl2  (C-X-C motif chemokine ligand 2)
Cxcl6  (C-X-C motif chemokine ligand 6)
Cxcr2  (C-X-C motif chemokine receptor 2)

Genes (Mus musculus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl2  (C-X-C motif chemokine ligand 2)
Cxcl5  (C-X-C motif chemokine ligand 5)
Cxcr2  (C-X-C motif chemokine receptor 2)

Genes (Homo sapiens)
CXCL1  (C-X-C motif chemokine ligand 1)
CXCL2  (C-X-C motif chemokine ligand 2)
CXCL5  (C-X-C motif chemokine ligand 5)
CXCR2  (C-X-C motif chemokine receptor 2)


Additional Information