RGD Reference Report - Adrenomedullin production is increased in colorectal adenocarcinomas; its relation to matrix metalloproteinase-9. - Rat Genome Database

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Adrenomedullin production is increased in colorectal adenocarcinomas; its relation to matrix metalloproteinase-9.

Authors: Hikosaka, Tomomi  Tsuruda, Toshihiro  Nagata, Sayaka  Kuwasako, Kenji  Tsuchiya, Kazuyo  Hoshiko, Shinri  Inatsu, Haruhiko  Chijiiwa, Kazuo  Kitamura, Kazuo 
Citation: Hikosaka T, etal., Peptides. 2011 Sep;32(9):1825-31. doi: 10.1016/j.peptides.2011.07.012. Epub 2011 Aug 3.
RGD ID: 152985531
Pubmed: PMID:21839130   (View Abstract at PubMed)
DOI: DOI:10.1016/j.peptides.2011.07.012   (Journal Full-text)

Adrenomedullin (AM) is highly expressed in various cancer cell lines, suggesting a possible association with cancer growth. In the present study, we examined the expression and/or concentration of AM, its related peptide, adrenomedullin2/intermedin (AM2/IMD) and their receptors in human colorectal cancer and the surrounding normal tissue. In addition, we assessed the correlation between the expression of AM and AM2/IMD with that of vascular endothelial growth factor (VEGF)-A and matrix metalloproteinase (MMP)-9. Using a specific immunoradiometric assay, we found that AM concentrations were 2-11-fold higher in colorectal cancer tissues than in the surrounding normal tissues. Moreover, real-time quantitative RT-PCR showed that the expression levels of preproAM (+548%), preproAM2/IMD (+2674%), calcitonin receptor-like receptor (CLR) (+518%), receptor activity modifying protein (RAMP)2 (+281%), RAMP3 (+178%), VEGF-A (+277%) and MMP-9 (+864%) mRNAs were significantly higher in cancer tissues than in the surrounding normal tissues, and there was a positive correlation between the gene expressions of MMP-9 and preproAM (r=0.352; p=0.005), but not with preproAM2/IMD (r=0.041, p=0.406). Both AM and AM2/IMD immunoreactivity were detected mainly within cancer cells, whereas MMP-9 immunoreactivity was mostly seen in the surrounding stroma. These findings suggest that AM produced in colorectal tumors acts in concert with MMP-9 in the stroma to contribute to the pathogenesis of colorectal cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ADMHumancolorectal cancer  IEP mRNA and protein:increased expression:colorectum (human)RGD 
AdmRatcolorectal cancer  ISOADM (Homo sapiens)mRNA and protein:increased expression:colorectum (human)RGD 
AdmMousecolorectal cancer  ISOADM (Homo sapiens)mRNA and protein:increased expression:colorectum (human)RGD 
MMP9Humancolorectal cancer  IEP mRNA:increased expresion:colorectum (human)RGD 
Mmp9Ratcolorectal cancer  ISOMMP9 (Homo sapiens)mRNA:increased expresion:colorectum (human)RGD 
Mmp9Mousecolorectal cancer  ISOMMP9 (Homo sapiens)mRNA:increased expresion:colorectum (human)RGD 
RAMP2Humancolorectal cancer  IEP mRNA:increased expression:colorectum (human)RGD 
RAMP3Humancolorectal cancer  IEP mRNA:increased expression:colorectum (human)RGD 
Ramp2Ratcolorectal cancer  ISORAMP2 (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 
Ramp2Mousecolorectal cancer  ISORAMP2 (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 
Ramp3Ratcolorectal cancer  ISORAMP3 (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 
Ramp3Mousecolorectal cancer  ISORAMP3 (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 
VEGFAHumancolorectal cancer  IEP mRNA:increased expression:colorectum (human)RGD 
VegfaRatcolorectal cancer  ISOVEGFA (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 
VegfaMousecolorectal cancer  ISOVEGFA (Homo sapiens)mRNA:increased expression:colorectum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Adm  (adrenomedullin)
Mmp9  (matrix metallopeptidase 9)
Ramp2  (receptor activity modifying protein 2)
Ramp3  (receptor activity modifying protein 3)
Vegfa  (vascular endothelial growth factor A)

Genes (Mus musculus)
Adm  (adrenomedullin)
Mmp9  (matrix metallopeptidase 9)
Ramp2  (receptor (calcitonin) activity modifying protein 2)
Ramp3  (receptor (calcitonin) activity modifying protein 3)
Vegfa  (vascular endothelial growth factor A)

Genes (Homo sapiens)
ADM  (adrenomedullin)
MMP9  (matrix metallopeptidase 9)
RAMP2  (receptor activity modifying protein 2)
RAMP3  (receptor activity modifying protein 3)
VEGFA  (vascular endothelial growth factor A)


Additional Information