RGD Reference Report - Expression and Prognostic Significance of EP300, TP53 and BAX in Clear Cell Renal Cell Carcinoma. - Rat Genome Database

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Expression and Prognostic Significance of EP300, TP53 and BAX in Clear Cell Renal Cell Carcinoma.

Authors: Godlewski, Janusz  Krazinski, Bartlomiej E  Kowalczyk, Anna E  Kiewisz, Jolanta  Kiezun, Jacek  Kwiatkowski, Przemyslaw  Sliwińska-Jewsiewicka, Agnieszka  Wierzbicki, Piotr W  Kmieć, Zbigniew 
Citation: Godlewski J, etal., Anticancer Res. 2017 Jun;37(6):2927-2937. doi: 10.21873/anticanres.11646.
RGD ID: 15036804
Pubmed: PMID:28551630   (View Abstract at PubMed)
DOI: DOI:10.21873/anticanres.11646   (Journal Full-text)


BACKGROUND: Histone acetyltransferase E1A-binding protein p300 (EP300), tumor protein p53 (TP53) and B-cell lymphoma-2-associated X protein (BAX) contribute to the regulation of the cell cycle and apoptosis, cellular processes that are often impaired in cancer cells. The aim of this study was to determine the expression levels of EP300, TP53 and BAX genes and their respective proteins in clear cell renal cell carcinoma (ccRCC) and evaluate the value of these factors as prognostic factors.
MATERIALS AND METHODS: EP300, TP53 and BAX expression at the transcript and protein levels were determined by quantitative polymerase-chain reaction (QPCR) and immunohistochemistry (IHC) in paired tumor and kidney specimens from 31 patients with ccRCC.
RESULTS: Levels of EP300, TP53 and BAX transcripts were found increased in tumor tissues. Immunoreactivity for TP53 was elevated in cancer cells when compared to unchanged kidney, while EP300 and BAX immunoexpression in ccRCC did not differ from that of normal renal tissue. Immunoreactivity for TP53 was positively associated with larger tumor size. In contrast, stronger BAX immunoexpression correlated with smaller tumor diameters. The average immunoreactivity for BAX was higher in localized, kidney-confined tumor than in advanced/recurrent tumors. None of the analyzed transcripts or proteins correlated with the overall survival of patients.
CONCLUSION: Although TP53 and BAX immunoreactivity levels were associated with some clinicopathological parameters of the patients, the expression of EP300, TP53 and BAX did not reveal any prognostic significance in ccRCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BAXHumanclear cell renal cell carcinoma  IEP mRNA and protein:increased expression:kidneyRGD 
BaxRatclear cell renal cell carcinoma  ISOBAX (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 
BaxMouseclear cell renal cell carcinoma  ISOBAX (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 
EP300Humanclear cell renal cell carcinoma  IEP mRNA and protein:increased expression:kidneyRGD 
Ep300Ratclear cell renal cell carcinoma  ISOEP300 (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 
Ep300Mouseclear cell renal cell carcinoma  ISOEP300 (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 
TP53Humanclear cell renal cell carcinoma  IEP mRNA and protein:increased expression:kidneyRGD 
Tp53Ratclear cell renal cell carcinoma  ISOTP53 (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 
Trp53Mouseclear cell renal cell carcinoma  ISOTP53 (Homo sapiens)mRNA and protein:increased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bax  (BCL2 associated X, apoptosis regulator)
Ep300  (E1A binding protein p300)
Tp53  (tumor protein p53)

Genes (Mus musculus)
Bax  (BCL2-associated X protein)
Ep300  (E1A binding protein p300)
Trp53  (transformation related protein 53)

Genes (Homo sapiens)
BAX  (BCL2 associated X, apoptosis regulator)
EP300  (E1A binding protein p300)
TP53  (tumor protein p53)


Additional Information