Targeted massively parallel sequencing of angiosarcomas reveals frequent activation of the mitogen activated protein kinase pathway. |
| Authors: |
Murali, Rajmohan Chandramohan, Raghu Möller, Inga Scholz, Simone L Berger, Michael Huberman, Kety Viale, Agnes Pirun, Mono Socci, Nicholas D Bouvier, Nancy Bauer, Sebastian Artl, Monika Schilling, Bastian Schimming, Tobias Sucker, Antje Schwindenhammer, Benjamin Grabellus, Florian Speicher, Michael R Schaller, Jörg Hillen, Uwe Schadendorf, Dirk Mentzel, Thomas Cheng, Donavan T Wiesner, Thomas Griewank, Klaus G
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| Citation: |
Murali R, etal., Oncotarget. 2015 Nov 3;6(34):36041-52. doi: 10.18632/oncotarget.5936. |
| RGD ID: |
151665099 |
| Pubmed: |
PMID:26440310 (View Abstract at PubMed) |
| PMCID: |
PMC4742160 (View Article at PubMed Central) |
| DOI: |
DOI:10.18632/oncotarget.5936 (Journal Full-text) |
Angiosarcomas are rare malignant mesenchymal tumors of endothelial differentiation. The clinical behavior is usually aggressive and the prognosis for patients with advanced disease is poor with no effective therapies. The genetic bases of these tumors have been partially revealed in recent studies reporting genetic alterations such as amplifications of MYC (primarily in radiation-associated angiosarcomas), inactivating mutations in PTPRB and R707Q hotspot mutations of PLCG1. Here, we performed a comprehensive genomic analysis of 34 angiosarcomas using a clinically-approved, hybridization-based targeted next-generation sequencing assay for 341 well-established oncogenes and tumor suppressor genes. Over half of the angiosarcomas (n = 18, 53%) harbored genetic alterations affecting the MAPK pathway, involving mutations in KRAS, HRAS, NRAS, BRAF, MAPK1 and NF1, or amplifications in MAPK1/CRKL, CRAF or BRAF. The most frequently detected genetic aberrations were mutations in TP53 in 12 tumors(35%) and losses of CDKN2A in9 tumors (26%). MYC amplifications were generally mutually exclusive of TP53 alterations and CDKN2A loss and were identified in 8 tumors (24%), most of which (n = 7, 88%) arose post-irradiation. Previously reported mutations in PTPRB (n = 10, 29%) and one (3%) PLCG1 R707Q mutation were also identified. Our results demonstrate that angiosarcomas are a genetically heterogeneous group of tumors, harboring a wide range of genetic alterations. The high frequency of genetic events affecting the MAPK pathway suggests that targeted therapies inhibiting MAPK signaling may be promising therapeutic avenues in patients with advanced angiosarcomas.
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| Genes (Rattus norvegicus) |
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| Myc (MYC proto-oncogene, bHLH transcription factor) | Ptprb (protein tyrosine phosphatase, receptor type, B) | Tp53 (tumor protein p53) |
| Genes (Mus musculus) |
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| Myc (myelocytomatosis oncogene) | Ptprb (protein tyrosine phosphatase receptor type B) | Trp53 (transformation related protein 53) |
| Genes (Homo sapiens) |
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| MYC (MYC proto-oncogene, bHLH transcription factor) | PTPRB (protein tyrosine phosphatase receptor type B) | TP53 (tumor protein p53) |
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