Any process that stops, prevents or reduces the frequency, rate or extent of DNA duplex unwinding.
Synonyms:
exact_synonym:
down regulation of DNA duplex unwinding; down regulation of DNA unwinding; down regulation of duplex DNA melting; downregulation of DNA duplex unwinding; downregulation of DNA unwinding; downregulation of duplex DNA melting; negative regulation of DNA unwinding; negative regulation of duplex DNA melting
narrow_synonym:
inhibition of DNA duplex unwinding; inhibition of DNA unwinding; inhibition of duplex DNA melting