RGD Reference Report - Thioredoxin and glutaredoxin system proteins-immunolocalization in the rat central nervous system. - Rat Genome Database
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Thioredoxin and glutaredoxin system proteins-immunolocalization in the rat central nervous system.

Authors: Aon-Bertolino, ML  Romero, JI  Galeano, P  Holubiec, M  Badorrey, MS  Saraceno, GE  Hanschmann, EM  Lillig, CH  Capani, F 
Citation: Aon-Bertolino ML, etal., Biochim Biophys Acta. 2011 Jan;1810(1):93-110. Epub 2010 Jul 8.
RGD ID: 5133712
Pubmed: (View Article at PubMed) PMID:20620191
DOI: Full-text: DOI:10.1016/j.bbagen.2010.06.011

BACKGROUND: The oxidoreductases of the thioredoxin (Trx) family of proteins play a major role in the cellular response to oxidative stress. Redox imbalance is a major feature of brain damage. For instance, neuronal damage and glial reaction induced by a hypoxic-ischemic episode is highly related to glutamate excitotoxicity, oxidative stress and mitochondrial dysfunction. Most animal models of hypoxia-ischemia in the central nervous system (CNS) use rats to study the mechanisms involved in neuronal cell death, however, no comprehensive study on the localization of the redox proteins in the rat CNS was available. METHODS: The aim of this work was to study the distribution of the following proteins of the thioredoxin and glutathione/glutaredoxin (Grx) systems in the rat CNS by immunohistochemistry: Trx1, Trx2, TrxR1, TrxR2, Txnip, Grx1, Grx2, Grx3, Grx5, and gamma-GCS, peroxiredoxin 1 (Prx1), Prx2, Prx3, Prx4, Prx5, and Prx6. We have focused on areas most sensitive to a hypoxia-ischemic insult: Cerebellum, striatum, hippocampus, spinal cord, substantia nigra, cortex and retina. RESULTS AND CONCLUSIONS: Previous studies implied that these redox proteins may be distributed in most cell types and regions of the CNS. Here, we have observed several remarkable differences in both abundance and regional distribution that point to a complex interplay and crosstalk between the proteins of this family. GENERAL SIGNIFICANCE: We think that these data might be helpful to reveal new insights into the role of thiol redox pathways in the pathogenesis of hypoxia-ischemia insults and other disorders of the CNS. This article is part of a Special Issue entitled Human and Murine Redox Protein Atlases.

Annotation

Gene Ontology Annotations    

Biological Process
aging  (IEP)

Cellular Component
axon  (IDA)
cytoplasm  (IDA)
dendrite  (IDA)
neuronal cell body  (IDA)
nucleus  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Glrx  (glutaredoxin)
Glrx2  (glutaredoxin 2)
Glrx3  (glutaredoxin 3)
Glrx5  (glutaredoxin 5)
Txn1  (thioredoxin 1)
Txn2  (thioredoxin 2)
Txnrd1  (thioredoxin reductase 1)
Txnrd2  (thioredoxin reductase 2)


Additional Information