RGD Reference Report - Plasma proteomic analysis may identify new markers for radiation-induced lung toxicity in patients with non-small-cell lung cancer. - Rat Genome Database

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Plasma proteomic analysis may identify new markers for radiation-induced lung toxicity in patients with non-small-cell lung cancer.

Authors: Cai, XW  Shedden, K  Ao, X  Davis, M  Fu, XL  Lawrence, TS  Lubman, DM  Kong, FM 
Citation: Cai XW, etal., Int J Radiat Oncol Biol Phys. 2010 Jul 1;77(3):867-76.
RGD ID: 5129484
Pubmed: PMID:20510197   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ijrobp.2010.01.038   (Journal Full-text)

PURPOSE: To study whether radiation induces differential changes in plasma proteomics in patients with and without radiation-induced lung toxicity (RILT) of Grade >/=2 (RILT2). METHODS AND MATERIALS: A total of 57 patients with NSCLC received radiation therapy (RT) were eligible. Twenty patients, 6 with RILT2 with tumor stage matched to 14 without RILT2, were enrolled for this analysis. Platelet-poor plasma was obtained before RT, at 2, 4, 6 weeks during RT, and 1 and 3 months after RT. Plasma proteomes were compared using a multiplexed quantitative proteomics approach involving ExacTag labeling, reverse-phase high-performance liquid chromatography and nano-LC electrospray tandem mass spectrometry. Variance components models were used to identify the differential protein expression between patients with and without RILT2. RESULTS: More than 100 proteins were identified and quantified. After excluding proteins for which there were not at least 2 subjects with data for at least two time points, 76 proteins remained for this preliminary analysis. C4b-binding protein alpha chain, Complement C3, and Vitronectin had significantly higher expression levels in patients with RILT2 compared with patients without RILT2, based on both the data sets of RT start to 3 months post-RT (p < 0.01) and RT start to the end of RT (p < 0.01). The expression ratios of patients with RILT2 vs. without RILT2 were 1.60, 1.36, 1.46, and 1.66, 1.34, 1.46, for the above three proteins, respectively. CONCLUSIONS: This proteomic approach identified new plasma protein markers for future studies on RILT prediction.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
C3RatRadiation Pneumonitis  ISOC3 (Homo sapiens)Protein: increased expression: plasmaRGD 
C3HumanRadiation Pneumonitis  IEP Protein: increased expression: plasmaRGD 
C3MouseRadiation Pneumonitis  ISOC3 (Homo sapiens)Protein: increased expression: plasmaRGD 
C4BPAHumanRadiation Pneumonitis  IEP Protein: increased expression: plasmaRGD 
C4bpMouseRadiation Pneumonitis  ISOC4BPA (Homo sapiens)Protein: increased expression: plasmaRGD 
C4bpaRatRadiation Pneumonitis  ISOC4BPA (Homo sapiens)Protein: increased expression: plasmaRGD 
VTNHumanRadiation Pneumonitis  IEP Protein: increased expression: plasmaRGD 
VtnRatRadiation Pneumonitis  ISOVTN (Homo sapiens)Protein: increased expression: plasmaRGD 
VtnMouseRadiation Pneumonitis  ISOVTN (Homo sapiens)Protein: increased expression: plasmaRGD 

Objects Annotated

Genes (Rattus norvegicus)
C3  (complement C3)
C4bpa  (complement component 4 binding protein, alpha)
Vtn  (vitronectin)

Genes (Mus musculus)
C3  (complement component 3)
C4bp  (complement component 4 binding protein)
Vtn  (vitronectin)

Genes (Homo sapiens)
C3  (complement C3)
C4BPA  (complement component 4 binding protein alpha)
VTN  (vitronectin)


Additional Information