RGD Reference Report - Expression of matrix metalloproteinase-26 and tissue inhibitors of metalloproteinases TIMP-3 and -4 in benign endometrium and endometrial cancer. - Rat Genome Database

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Expression of matrix metalloproteinase-26 and tissue inhibitors of metalloproteinases TIMP-3 and -4 in benign endometrium and endometrial cancer.

Authors: Tunuguntla, R  Ripley, D  Sang, QX  Chegini, N 
Citation: Tunuguntla R, etal., Gynecol Oncol. 2003 Jun;89(3):453-9.
RGD ID: 2290420
Pubmed: PMID:12798711   (View Abstract at PubMed)

OBJECTIVE: Matrix metalloproteinases (MMPs) and their physiological inhibitors, the tissue inhibitors of MMPs (TIMPs), play a key role in tumor cell invasion, angiogenesis, and growth. The aim of this study was to determine the expression and cellular distribution of MMP-26, TIMP-3, and TIMP-4 in endometrial cancers and benign endometrium throughout the menstrual cycle and the correlation with tumor histological subtype, stage, and grade. METHODS: Immunohistochemical analysis using polyclonal antibodies generated against pro- and active MMP-26, and mono- and polyclonal antibodies specific to TIMP-3 and TIMP-4, respectively, was performed. RESULTS: MMP-26, TIMP-3, and TIMP-4 are expressed in endometrial carcinomas (N = 86) and benign endometrium (N = 50) from various stages of the menstrual cycle. Semi-quantitative analysis of staining intensity indicated that endometrial carcinomas expressed more MMP-26, TIMP-3, and TIMP-4 compared to benign endometrium from the postmenopausal period, but not from the secretory phase of the menstrual cycle. The highest staining intensity was associated with endometrial epithelial cells, followed by vascular endothelial cells, myometrial smooth muscle cells, and endometrial stromal cells. Increased staining intensity of MMP-26 and TIMP-3 correlated with grade III tumors and MMP-26 and TIMP-4 with the depth of myometrial invasion in tumors histologically characterized as endometrioid adenocarcinoma, clear-cell, and papillary serous carcinoma staged/graded based on FIGO criteria. CONCLUSION: MMP-26 and TIMP-4 are expressed in endometrium and endometrial carcinoma and their elevated expression and correlation with myometrial invasion suggests that MMP-26 and TIMP-4 may play a key role in endometrial tumor progression.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endometrial carcinoma disease_progressionIEP 2290420protein:increased expression:endometriumRGD 
endometrial carcinoma disease_progressionISOTIMP4 (Homo sapiens)2290420; 2290420protein:increased expression:endometriumRGD 
Endometrial Neoplasms disease_progressionIEP 2290420protein:increased expression:endometriumRGD 
Endometrial Neoplasms disease_progressionISOTIMP3 (Homo sapiens)2290420; 2290420protein:increased expression:endometriumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Timp3  (TIMP metallopeptidase inhibitor 3)
Timp4  (TIMP metallopeptidase inhibitor 4)

Genes (Mus musculus)
Timp3  (tissue inhibitor of metalloproteinase 3)
Timp4  (tissue inhibitor of metalloproteinase 4)

Genes (Homo sapiens)
TIMP3  (TIMP metallopeptidase inhibitor 3)
TIMP4  (TIMP metallopeptidase inhibitor 4)


Additional Information