RGD Reference Report - Confirmation of quantitative trait loci for ethanol sensitivity and neurotensin receptor density in crosses derived from the inbred high and low alcohol sensitive selectively bred rat lines. - Rat Genome Database

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Confirmation of quantitative trait loci for ethanol sensitivity and neurotensin receptor density in crosses derived from the inbred high and low alcohol sensitive selectively bred rat lines.

Authors: Radcliffe, RA  Bludeau, P  Asperi, W  Fay, T  Deng, XS  Erwin, VG  Deitrich, RA 
Citation: Radcliffe RA, etal., Psychopharmacology (Berl). 2006 Oct;188(3):343-54. Epub 2006 Sep 5.
RGD ID: 1600610
Pubmed: PMID:16953387   (View Abstract at PubMed)
DOI: DOI:10.1007/s00213-006-0512-2   (Journal Full-text)

RATIONALE: Genetically influenced alcohol sensitivity is thought to be an important risk factor for the development of alcoholism. An effective first step for identifying genes that mediate variation in alcohol sensitivity is through quantitative trait loci (QTL) mapping in model organisms. OBJECTIVE: Fourteen provisional QTLs related to alcohol sensitivity were previously mapped in an F2 derived from the IHAS1 and ILAS1 rat lines. The objective of the current study was to confirm those QTLs in an independently derived F2 and in congenics that were bred for two of the loci. MATERIALS AND METHODS: IHAS1 X ILAS1 F2 (n=450) were tested for alcohol-induced loss of righting reflex (LORR), blood ethanol concentration at regain of righting reflex (BECRR), sensitivity and acute tolerance on the Rotarod, and neurotensin receptor density (NTR1). Rats were genotyped at the 14 candidate loci and QTL mapping was conducted. Reciprocal congenic strains were bred for loci on chromosomes 2 and 5 and tested for LORR and BECRR. RESULTS: Four LORR QTLs were mapped at the suggestive or significant level (chromosomes 2, 5, 12, and 13). BECRR was mapped to chromosomes 5, 12, and 13 either in the original or current experiment. Results of the congenic experiment also support QTLs for LORR and BECRR on chromosomes 2 and 5. QTLs for NTR1 density and behavior on the Rotarod were not confirmed. CONCLUSIONS: QTL mapping in crosses derived from the IHAS1 and ILAS1 has successfully identified loci related to alcohol sensitivity. Recombinant congenics are now being bred to more finely map the confirmed QTLs.

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
improved righting response sexual_dimorphismIAGPethanol1600610compared to female HAS1RGD 
improved righting response sexual_dimorphismIAGPethanol1600610compared to female HAS1RGD 
Objects referenced in this article
QTL Alcrsp11 Alcohol response QTL 11 Rattus norvegicus
QTL Alcrsp12 Alcohol response QTL 12 Rattus norvegicus
QTL Alcrsp13 Alcohol response QTL 13 Rattus norvegicus
QTL Alcrsp14 Alcohol response QTL 14 Rattus norvegicus
QTL Alcrsp15 Alcohol response QTL 16 Rattus norvegicus
QTL Alcrsp16 Alcohol response QTL 16 Rattus norvegicus
QTL Alcrsp17 Alcohol response QTL 17 Rattus norvegicus
QTL Alcrsp18 Alcohol response QTL 18 Rattus norvegicus
QTL Alcrsp8 Alcohol response QTL 8 Rattus norvegicus
Strain HAS1 High Alcohol Sensitive strain 1 Rattus norvegicus
Strain LAS1 Low Alcohol Sensitive strain 1 Rattus norvegicus

Additional Information