RGD Reference Report - Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion. - Rat Genome Database

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Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion.

Authors: Xia, W  Mruk, DD  Lee, WM  Cheng, CY 
Citation: Xia W, etal., J Biol Chem. 2006 Jun 16;281(24):16799-813. Epub 2006 Apr 13.
RGD ID: 1582382
Pubmed: PMID:16617054   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M601618200   (Journal Full-text)

The biochemical basis that regulates the timely and selective opening of the blood-testis barrier (BTB) to migrating preleptotene/leptotene spermatocytes at stage VIII of the epithelial cycle in adult rat testes is virtually unknown. Recent studies have shown that cytokines (e.g. transforming growth factor (TGF)-beta3) may play a crucial role in this event. However, much of this information relies on the use of toxicants (e.g. CdCl(2)), making it difficult to relay these findings to normal testicular physiology. Here we report that overexpression of TGF-beta3 in primary Sertoli cells cultured in vitro indeed perturbed the tight junction (TJ) barrier with a concomitant decline in the production of BTB constituent proteins as follows: occludin, N-cadherin, and ZO-1. Additionally, local administration of TGF-beta3 to testes in vivo was shown to reversibly perturb the BTB integrity and Sertoli-germ cell adhesion via the p38 MAPK and ERK signaling pathways. Most importantly, the simultaneous activation of p38 and ERK signaling pathways is dependent on the association of the TGF-beta3-TbetaR1 complex with adaptors TAB1 and CD2AP because if TbetaR1 was associated preferentially with CD2AP, only Sertoli-germ cell adhesion was perturbed without compromising the BTB. Collectively, these data illustrate that local production of TGF-beta3, and perhaps other TGF-betas and cytokines, by Sertoli and germ cells into the microenvironment at the BTB during spermatogenesis transiently perturbs the BTB and Sertoli-germ cell adhesion to facilitate germ cell migration when the activated TbetaRI interacts with adaptors TAB1 and CD2AP. However, TGF-beta3 selectively disrupts Sertoli-germ cell adhesion in the seminiferous epithelium to facilitate germ cell migration without compromising BTB when TbetaRI interacts only with adaptor CD2AP.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cd2apRatcell-cell adhesion  IMP  RGD 

Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cd2apRatprotein-containing complex  IDA  RGD 
Tab1Ratprotein-containing complex  IDA  RGD 
Tgfbr1Ratprotein-containing complex  IDA  RGD 


Genes (Rattus norvegicus)
Cd2ap  (CD2-associated protein) Cdh2  (cadherin 2) F11r  (F11 receptor)
Tab1  (TGF-beta activated kinase 1/MAP3K7 binding protein 1) Tgfbr1  (transforming growth factor, beta receptor 1) Tjp1  (tight junction protein 1)

Gene Ctnnb1 catenin beta 1 Rattus norvegicus