RGD Reference Report - Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury.

Authors: An, JL  Ishida, Y  Kimura, A  Tsokos, M  Kondo, T 
Citation: An JL, etal., Forensic Sci Int. 2009 Nov 20;192(1-3):e21-5. Epub 2009 Sep 4.
RGD ID: 4144897
Pubmed: PMID:19733456   (View Abstract at PubMed)
DOI: DOI:10.1016/j.forsciint.2009.08.007   (Journal Full-text)

Sepsis is a systemic inflammatory disease with high mortality. In the present study, we immunohistochemically examined CCR2 and CX3CR1 expression in sepsis-induced lung injury, and discussed its availability for the postmortem diagnosis of sepsis. Lung samples were obtained from different lung lobes of nine sepsis and eight control cases with postmortem intervals between 12 and 48h. Immunohistochemically, mononuclear cells recruited into the lungs expressed CCR2 and CX3CR1 in both sepsis and non-septic groups. In double-color immunofluorescence analysis, CCR2- or CX3CR1-positive cells could be identified as CD68-positive macrophages. Moreover, most of CD68-positive macrophages expressed both CCR2 and CX3CR1. Morphometrically, the average of CCR2- and CX3CR1-positive macrophages was significantly increased in sepsis group, compared with control group (sepsis vs. control: 41.6+/-1.3% vs. 8.0+/-0.4% in CCR2; 36.2+/-1.3% vs. 9.2+/-0.4% in CX3CR1). These observations implied that CCR2- or CX3CR1-positive macrophages were recruited into the lungs under several pathological conditions. In particular, their recruitment might be more evident in sepsis. Moreover, from the forensic aspects, immunohistochemical detection of CCR2 and CX3CR1 expression in the lungs can be considered as valuable diagnostic tools for the postmortem diagnosis of sepsis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CCR2Humanlung disease  IEP lung injury associated with sepsis and protein:increased expression:macrophage alveolarRGD 
Ccr2Ratlung disease  ISOCCR2 (Homo sapiens)lung injury associated with sepsis and protein:increased expression:macrophage alveolarRGD 
Ccr2Mouselung disease  ISOCCR2 (Homo sapiens)lung injury associated with sepsis and protein:increased expression:macrophage alveolarRGD 
CX3CR1HumanSepsis  IEP protein:increased expression:lung and macrophageRGD 
Cx3cr1RatSepsis  ISOCX3CR1 (Homo sapiens)protein:increased expression:lung and macrophageRGD 
Cx3cr1MouseSepsis  ISOCX3CR1 (Homo sapiens)protein:increased expression:lung and macrophageRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccr2  (C-C motif chemokine receptor 2)
Cx3cr1  (C-X3-C motif chemokine receptor 1)

Genes (Mus musculus)
Ccr2  (C-C motif chemokine receptor 2)
Cx3cr1  (C-X3-C motif chemokine receptor 1)

Genes (Homo sapiens)
CCR2  (C-C motif chemokine receptor 2)
CX3CR1  (C-X3-C motif chemokine receptor 1)


Additional Information