RGD Reference Report - Propofol reduces nitric oxide-induced apoptosis in testicular ischemia-reperfusion injury by downregulating the expression of inducible nitric oxide synthase. - Rat Genome Database

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Propofol reduces nitric oxide-induced apoptosis in testicular ischemia-reperfusion injury by downregulating the expression of inducible nitric oxide synthase.

Authors: Yagmurdur, H  Ayyildiz, A  Karaguzel, E  Akgul, T  Ustun, H  Germiyanoglu, C 
Citation: Yagmurdur H, etal., Acta Anaesthesiol Scand. 2008 Mar;52(3):350-7. Epub 2008 Jan 16.
RGD ID: 2292105
Pubmed: PMID:18205898   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1399-6576.2007.01545.x   (Journal Full-text)

BACKGROUND: The aim of the present study was to investigate the underlying mechanisms in the preventive effects of intravenous anesthetics on testicular ischemia-reperfusion injury. METHODS: Forty male Wistar Albino rats were randomly assigned to four groups of 10 rats each. Anesthesia was induced and maintained with thiopental in groups 1 and 2 and with propofol in groups 3 and 4. Groups 2 and 4 received left testicular ischemia (torsion) for 1 h and reperfusion (detorsion) for 24 h. Groups 1 and 3 (control groups) had no testicular torsion and detorsion. At 24 h of reperfusion, animals were killed and ipsilateral testes were removed for determination of tissue nitric oxide (NO) levels and immunohistochemical evaluation of endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and apoptosis protease-activating factor 1 (APAF-1). RESULTS: Between groups 1 and 3, there were no differences in tissue NO levels and eNOS, iNOS, and APAF-1 expressions. iNOS and APAF-1 expressions were markedly increased in group 2, but these parameters were at the mild to moderate level in group 4 at 24 h of reperfusion. Also, elevated expression of iNOS was accompanied by a high NO production in group 2 compared with group 4. Although eNOS expressions were increased in both the groups (groups 2 and 4), there were no significant differences between these groups. CONCLUSIONS: Propofol as an anesthetic agent may attenuate germ cell-specific apoptosis and decrease NO biosynthases through downregulation of iNOS expression in an animal model of testicular torsion and detorsion.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
APAF1HumanReperfusion Injury  ISOApaf1 (Rattus norvegicus) RGD 
Apaf1RatReperfusion Injury  IEP  RGD 
Apaf1MouseReperfusion Injury  ISOApaf1 (Rattus norvegicus) RGD 
NOS3HumanTestis Reperfusion Injury  ISONos3 (Rattus norvegicus)protein:increased expression:testisRGD 
Nos3RatTestis Reperfusion Injury  IEP protein:increased expression:testisRGD 
Nos3MouseTestis Reperfusion Injury  ISONos3 (Rattus norvegicus)protein:increased expression:testisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Apaf1  (apoptotic peptidase activating factor 1)
Nos3  (nitric oxide synthase 3)

Genes (Mus musculus)
Apaf1  (apoptotic peptidase activating factor 1)
Nos3  (nitric oxide synthase 3, endothelial cell)

Genes (Homo sapiens)
APAF1  (apoptotic peptidase activating factor 1)
NOS3  (nitric oxide synthase 3)


Additional Information