RGD Reference Report - Growth suppression of colorectal cancer by plant-derived multiple mAb CO17-1A × BR55 via inhibition of ERK1/2 phosphorylation. - Rat Genome Database

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Growth suppression of colorectal cancer by plant-derived multiple mAb CO17-1A × BR55 via inhibition of ERK1/2 phosphorylation.

Authors: Kwak, Dong Hoon  Moussavou, Ghislain  Lee, Ju Hyoung  Heo, Sung Youn  Ko, Kisung  Hwang, Kyung-A  Jekal, Seung-Joo  Choo, Young-Kug 
Citation: Kwak DH, etal., Int J Mol Sci. 2014 Nov 14;15(11):21105-19. doi: 10.3390/ijms151121105.
RGD ID: 13210782
Pubmed: PMID:25405740   (View Abstract at PubMed)
PMCID: PMC4264215   (View Article at PubMed Central)
DOI: DOI:10.3390/ijms151121105   (Journal Full-text)

We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAb(P)) CO17-1A and mAb(P) CO17-1A × BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAb(P) CO17-1A × BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAb(P) CO17-1A × BR55-treated. The mAb(P) CO17-1A × BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased. In vivo, the mAb(P) CO17-1A × BR55 significantly and efficiently inhibited the growth of colon tumors compared to another mAbs. The apoptotic cell death and inhibition of pro-apoptotic proteins expression were highest by treatment with mAb(P) CO17-1A × BR55. In addition, the mAb(P) CO17-1A × BR55 significantly inhibited the extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation in cancer cells and tumors. Therefore, this study results suggest that multiple mAb(P) CO17-1A × BR55 has a significant effect on apoptosis-mediated anticancer by suppression of ERK1/2 phosphorylation in colon cancer compared to another mAbs. In light of these results, further clinical investigation should be conducted on mAb(P) CO17-1A × BR55 to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MAPK1Humancolorectal adenocarcinoma treatmentIDA  RGD 
MAPK3Humancolorectal adenocarcinoma treatmentIDA  RGD 
Mapk1Ratcolorectal adenocarcinoma treatmentISOMAPK1 (Homo sapiens) RGD 
Mapk1Mousecolorectal adenocarcinoma treatmentISOMAPK1 (Homo sapiens) RGD 
Mapk3Ratcolorectal adenocarcinoma treatmentISOMAPK3 (Homo sapiens) RGD 
Mapk3Mousecolorectal adenocarcinoma treatmentISOMAPK3 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mapk1  (mitogen activated protein kinase 1)
Mapk3  (mitogen activated protein kinase 3)

Genes (Mus musculus)
Mapk1  (mitogen-activated protein kinase 1)
Mapk3  (mitogen-activated protein kinase 3)

Genes (Homo sapiens)
MAPK1  (mitogen-activated protein kinase 1)
MAPK3  (mitogen-activated protein kinase 3)


Additional Information