RGD Reference Report - 7,3'-dimethoxy hesperetin inhibits inflammation by inducing synovial apoptosis in rats with adjuvant-induced arthritis. - Rat Genome Database

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7,3'-dimethoxy hesperetin inhibits inflammation by inducing synovial apoptosis in rats with adjuvant-induced arthritis.

Authors: Li, R  Cai, L  Xie, XF  Peng, L  Wu, TN  Li, J 
Citation: Li R, etal., Immunopharmacol Immunotoxicol. 2013 Feb;35(1):139-46. doi: 10.3109/08923973.2012.723010. Epub 2012 Sep 17.
RGD ID: 10054498
Pubmed: PMID:22978269   (View Abstract at PubMed)
DOI: DOI:10.3109/08923973.2012.723010   (Journal Full-text)

Rheumatoid arthritis (RA) is an autoimmune disease characterized by pronounced inflammation and excessive synovial hyperplasia within affected joint. We previously reported 7, 3'-dimethoxy hesperetin (DMHP) as a highly anti-inflammatory active derivative of hesperidin showed apparent pro-apoptotic effect in vitro on fibroblast-like synoviocytes of rats with adjuvant arthritis (AA), an animal model of RA. Here, we investigated the therapeutic effects of DMHP on inflammation and synovial apoptosis in rats with AA in vivo. Paw swelling, arthritis index, TNF-alpha and IL-1beta serum levels were measured to evaluate the effect of DMHP on inflammation in AA rats. DNA ladder detection and TUNEL assay were used to investigate the pro-apoptotic effect of DMHP on synovial apoptosis in vivo. Bcl-2, Bax mRNA and protein expressions in synovium were determined by real-time Q-PCR and western blot, respectively. We found DMHP inhibited secondary hind paw swelling and arthritis index, and decreased TNF-alpha and IL-1beta serum levels in AA rats. Typical DNA ladder formation was found in DNA extraction of synovium from DMHP treated groups. The number of apoptotic synovial cells was elevated with DMHP treatment in TUNEL assay. DMHP markedly decreased Bcl-2 expression whereas increased Bax expression in synovium of AA rats at both transcription and protein levels. Moreover, DMHP treatment on AA rats significantly decreased the protein ratio of Bcl-2/Bax in synovium. In conclusion, DMHP has an apparent therapeutic effect on inflammation in rats with AA. Mechanisms of this effect are partly related to induction of synovial apoptosis through modulation of Bcl-2 and Bax expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BAXHumanExperimental Arthritis treatmentISOBax (Rattus norvegicus) RGD 
BCL2HumanExperimental Arthritis treatmentISOBcl2 (Rattus norvegicus) RGD 
BaxRatExperimental Arthritis treatmentIEP  RGD 
BaxMouseExperimental Arthritis treatmentISOBax (Rattus norvegicus) RGD 
Bcl2RatExperimental Arthritis treatmentIEP  RGD 
Bcl2MouseExperimental Arthritis treatmentISOBcl2 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bax  (BCL2 associated X, apoptosis regulator)
Bcl2  (BCL2, apoptosis regulator)

Genes (Mus musculus)
Bax  (BCL2-associated X protein)
Bcl2  (B cell leukemia/lymphoma 2)

Genes (Homo sapiens)
BAX  (BCL2 associated X, apoptosis regulator)
BCL2  (BCL2 apoptosis regulator)


Additional Information