RGD Reference Report - Increased expression of gelatinase (MMP-2 and MMP-9) in pterygia and pterygium fibroblasts with disease progression and activation of protein kinase C.

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Increased expression of gelatinase (MMP-2 and MMP-9) in pterygia and pterygium fibroblasts with disease progression and activation of protein kinase C.
Authors:	Yang, SF Lin, CY Yang, PY Chao, SC Ye, YZ Hu, DN
Citation:	Yang SF, etal., Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4588-96. doi: 10.1167/iovs.08-3147. Epub 2009 May 6.
RGD ID:	8657043
Pubmed:	PMID:19420332 (View Abstract at PubMed)
DOI:	DOI:10.1167/iovs.08-3147 (Journal Full-text)
PURPOSE: To study the expression of matrix metalloprotease (MMP)-2 and MMP-9 mRNA and activities in various stages of surgically excised pterygium specimens and cultured pterygium fibroblasts and to study the effects of activation of protein kinase C (PKC) on the expression of these MMPs in pterygium fibroblasts. METHODS: MMP-2 and MMP-9 mRNA expression and activities in 15 pterygium tissues and cultured pterygium fibroblasts were measured by RT-PCR and zymography. Five normal conjunctiva specimens and fibroblasts were tested as the controls. Changes of expression of MMP-2 and MMP-9 of fibroblasts after the simulation of a standard PKC activator, 2-O-tetradecanoyl-phorbol-13-acetate (TPA), were studied. RESULTS: MMP-2 and MMP-9 expression in pterygium tissues and fibroblasts was greater than those of normal tissues and fibroblasts and was closely relevant to the progression of pterygium. In early-stage pterygium tissues and cultured fibroblasts, MMP-9 was not expressed, activated MMP-2 could not be detected, and only a small amount of latent MMP-2 was present. In advanced-stage pterygium (pterygium head passed the papillary region), MMP-9 was expressed; activated MMP-2 and a large amount of latent MMP-2 could be detected in pterygium tissues and fibroblasts. TPA stimulated the expression of MMP-2 and MMP-9 by pterygium fibroblasts isolated from early-stage specimens in a dose-dependent manner. CONCLUSIONS: MMP-2 and MMP-9 expression by pterygium fibroblasts is significantly increased after the progression of pterygium. Activation of the PKC signaling pathway, aside from other previously reported signaling pathways, may play a role in the development and progression of pterygium.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
MMP2	Human	pterygium	disease_progression	IDA			RGD
MMP9	Human	pterygium	disease_progression	IEP			RGD
Mmp2	Rat	pterygium	disease_progression	ISO	MMP2 (Homo sapiens)		RGD
Mmp2	Mouse	pterygium	disease_progression	ISO	MMP2 (Homo sapiens)		RGD
Mmp9	Rat	pterygium	disease_progression	ISO	MMP9 (Homo sapiens)		RGD
Mmp9	Mouse	pterygium	disease_progression	ISO	MMP9 (Homo sapiens)		RGD

Objects Annotated

Genes (Rattus norvegicus)

Mmp2 (matrix metallopeptidase 2)

Mmp9 (matrix metallopeptidase 9)

Genes (Mus musculus)

Mmp2 (matrix metallopeptidase 2)

Mmp9 (matrix metallopeptidase 9)

Genes (Homo sapiens)

MMP2 (matrix metallopeptidase 2)

MMP9 (matrix metallopeptidase 9)

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Increased expression of gelatinase (MMP-2 and MMP-9) in pterygia and pterygium fibroblasts with disease progression and activation of protein kinase C.