RGD Reference Report - Altered cytokine balance in the tear fluid and conjunctiva of patients with Sjogren's syndrome keratoconjunctivitis sicca. - Rat Genome Database

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Altered cytokine balance in the tear fluid and conjunctiva of patients with Sjogren's syndrome keratoconjunctivitis sicca.

Authors: Pflugfelder, SC  Jones, D  Ji, Z  Afonso, A  Monroy, D 
Citation: Pflugfelder SC, etal., Curr Eye Res. 1999 Sep;19(3):201-11.
RGD ID: 7829756
Pubmed: PMID:10487957   (View Abstract at PubMed)

PURPOSE: To compare epidermal growth factor (EGF) concentration in tear fluid and levels of inflammatory cytokines in the conjunctival epithelium of patients with Sjogren's syndrome keratoconjunctivitis sicca with those of normal controls. METHODS: Schirmer 1 tear testing, corneal fluorescein staining and conjunctival impression cytology for quantitation of goblet cell density were performed in ten patients with Sjogren's syndrome-associated keratoconjunctivitis sicca and ten asymptomatic normal controls. ELISA was used to detect the concentration of EGF in tear fluid and interleukin 6 in lysates of conjunctival cytology specimens obtained from all subjects. The levels of RNA transcripts encoding inflammatory cytokines [interleukin 1alpha_(IL-1alpha), interleukin 6 (IL-6), interleukin 8 (IL-8), tumor necrosis factor alpha_(TNF-alpha), and transforming growth factor beta1 (TGF-beta1)] as well as a housekeeping gene (G3PDH) were evaluated in conjunctival cytology specimens taken from all subjects by semiquantitative competitive reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Decreased tear fluid EGF concentration was noted in Sjogren's syndrome patients (mean 0.68 +/- 0.59 ng/ml) compared to controls (mean 1.66 +/- 0.45 ng/ml, P = 0.004). Significantly increased levels of IL-1alpha, IL-6, IL-8, TNF-alpha and TGF-beta1 RNA transcripts were found in the conjunctival epithelium of Sjogren's syndrome patients compared to controls (P < 0.05), while the level of G3PDH was similar in both groups. The concentration of IL-6 protein was significantly higher in Sjogren's syndrome conjunctiva samples (P = 0.012). Tear EGF concentration correlated with Schirmer 1 scores (rho 0.767, P < 0.001), corneal fluorescein staining scores (rho -0.562, P = 0.01), conjunctival goblet cell density (rho 0.661, P = 0.001) and the levels of IL-1alpha_and IL-8 RNA in the conjunctival epithelium (rho -0.677 and -0.747, respectively, P = 0.001). Both IL-1alpha_and IL-8 RNA in the conjunctival epithelium increased as Schirmer 1 scores decreased (P



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL6Humankeratoconjunctivitis sicca  IEP associated with Sjogren's Syndrome more ...RGD 
Il6Ratkeratoconjunctivitis sicca  ISOIL6 (Homo sapiens)associated with Sjogren's Syndrome more ...RGD 
Il6Mousekeratoconjunctivitis sicca  ISOIL6 (Homo sapiens)associated with Sjogren's Syndrome more ...RGD 
TNFHumankeratoconjunctivitis sicca  IEP associated with Sjogren's Syndrome and mRNA:increased expression:conjunctiva:RGD 
TnfRatkeratoconjunctivitis sicca  ISOTNF (Homo sapiens)associated with Sjogren's Syndrome and mRNA:increased expression:conjunctiva:RGD 
TnfMousekeratoconjunctivitis sicca  ISOTNF (Homo sapiens)associated with Sjogren's Syndrome and mRNA:increased expression:conjunctiva:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il6  (interleukin 6)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Il6  (interleukin 6)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
IL6  (interleukin 6)
TNF  (tumor necrosis factor)


Additional Information