RGD Reference Report - The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module. - Rat Genome Database

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The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module.

Authors: Rosenthal, JA  Chen, H  Slepnev, VI  Pellegrini, L  Salcini, AE  Di Fiore, PP  De Camilli, P 
Citation: Rosenthal JA, etal., J Biol Chem 1999 Nov 26;274(48):33959-65.
RGD ID: 708327
Pubmed: PMID:10567358   (View Abstract at PubMed)

Epsin (epsin 1) is an interacting partner for the EH domain-containing region of Eps15 and has been implicated in conjunction with Eps15 in clathrin-mediated endocytosis. We report here the characterization of a similar protein (epsin 2), which we have cloned from human and rat brain libraries. Epsin 1 and 2 are most similar in their NH(2)-terminal region, which represents a module (epsin NH(2) terminal homology domain, ENTH domain) found in a variety of other proteins of the data base. The multiple DPW motifs, typical of the central region of epsin 1, are only partially conserved in epsin 2. Both proteins, however, interact through this central region with the clathrin adaptor AP-2. In addition, we show here that both epsin 1 and 2 interact with clathrin. The three NPF motifs of the COOH-terminal region of epsin 1 are conserved in the corresponding region of epsin 2, consistent with the binding of both proteins to Eps15. Epsin 2, like epsin 1, is enriched in brain, is present in a brain-derived clathrin-coated vesicle fraction, is concentrated in the peri-Golgi region and at the cell periphery of transfected cells, and partially colocalizes with clathrin. High overexpression of green fluorescent protein-epsin 2 mislocalizes components of the clathrin coat and inhibits clathrin-mediated endocytosis. The epsins define a new protein family implicated in membrane dynamics at the cell surface.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Epn2Ratregulation of endocytosis  IDA  RGD 

Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Epn2Ratclathrin coat of endocytic vesicle  IDA  RGD 


Genes (Rattus norvegicus)
Epn2  (epsin 2)

Gene EPN2 epsin 2 Homo sapiens
Gene Amph amphiphysin Rattus norvegicus
Gene Cltc clathrin heavy chain Rattus norvegicus
Gene Epn1 Epsin 1 Rattus norvegicus