RGD Reference Report - Rhinovirus infection increases 5-lipoxygenase and cyclooxygenase-2 in bronchial biopsy specimens from nonatopic subjects. - Rat Genome Database

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Rhinovirus infection increases 5-lipoxygenase and cyclooxygenase-2 in bronchial biopsy specimens from nonatopic subjects.

Authors: Seymour, ML  Gilby, N  Bardin, PG  Fraenkel, DJ  Sanderson, G  Penrose, JF  Holgate, ST  Johnston, SL  Sampson, AP 
Citation: Seymour ML, etal., J Infect Dis. 2002 Feb 15;185(4):540-4. Epub 2002 Jan 31.
RGD ID: 4890421
Pubmed: PMID:11865407   (View Abstract at PubMed)
DOI: DOI:10.1086/338570   (Journal Full-text)

Rhinovirus infections cause wheeze, cough, and bronchial hyperresponsiveness. To investigate the involvement of cysteinyl-leukotrienes and prostanoids in these symptoms, bronchial biopsy specimens from 9 normal subjects (nonatopic and with no history of chronic lung disease) were immunostained for 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathway enzymes 2 weeks before and 4 days after experimental infection with human rhinovirus serotype 16. 5-LO-positive cell counts increased 9-fold (from 0.48 to 4.4 cells/mm(2); P <.05), and 5-LO-activating protein (FLAP)-positive cell counts increased 3.6-fold (from 1.8 to 6.5 cells/mm(2); P =.09). Levels of leukotriene A(4) hydrolase and leukotriene C(4) synthase were unchanged. COX-2--positive cell counts increased from 0 to 2.6 cells/mm(2) (P =.009), with no change in COX-1 levels. Increases of 3-4-fold were seen in levels of macrophages (P =.02) and mast cells (P =.07) but not of eosinophils (P >.4), and bronchoalveolar lavage fluid cysteinyl-leukotriene levels doubled (from 11.2 to 20.4 pg/mL; P =.13). Cold symptom scores correlated with bronchial immunostaining for FLAP (rho = 0.93; P =.001). In normal subjects, rhinovirus colds induce bronchial inflammation with markedly enhanced expression of 5-LO pathway proteins and COX-2.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ALOX5Humancommon cold  IEP protein:increased expression:bronchusRGD 
ALOX5APHumancommon cold  IEP protein:increased expression:bronchusRGD 
Alox5Ratcommon cold  ISOALOX5 (Homo sapiens)protein:increased expression:bronchusRGD 
Alox5Mousecommon cold  ISOALOX5 (Homo sapiens)protein:increased expression:bronchusRGD 
Alox5apRatcommon cold  ISOALOX5AP (Homo sapiens)protein:increased expression:bronchusRGD 
Alox5apMousecommon cold  ISOALOX5AP (Homo sapiens)protein:increased expression:bronchusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Mus musculus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Homo sapiens)
ALOX5  (arachidonate 5-lipoxygenase)
ALOX5AP  (arachidonate 5-lipoxygenase activating protein)


Additional Information