RGD Reference Report - Vasopressin V(2)-receptor-dependent regulation of AQP2 expression in Brattleboro rats. - Rat Genome Database

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Vasopressin V(2)-receptor-dependent regulation of AQP2 expression in Brattleboro rats.

Authors: Promeneur, D  Kwon, TH  Frokiaer, J  Knepper, MA  Nielsen, S 
Citation: Promeneur D, etal., Am J Physiol Renal Physiol. 2000 Aug;279(2):F370-82.
RGD ID: 2314654
Pubmed: PMID:10919858   (View Abstract at PubMed)

The role of AVP-V(2) receptor (AVP-V(2)R)-dependent regulation of aquaporin-2 (AQP2) expression was evaluated in vasopressin-deficient Brattleboro (BB) rats. AQP2 levels were relatively high in BB rats (52 +/- 8% of levels in Wistar rats), and treatment with the AVP-V(2)R antagonist SR-121463A (0.8 mg/day) for 48 h was associated with 1) increased urine output (170 +/- 9%), 2), reduced AQP2 protein levels (42 +/- 10% in whole kidney and 53 +/- 8% in inner medulla), and 3) reduced AQP2 mRNA levels (36 +/- 7%). In addition, the levels of AQP2 phosphorylated in the protein kinase A (PKA) consensus site (Ser(256) of AQP2) was reduced to 3 +/- 1% of control levels. Lithium (Li) treatment of BB rats for 1 mo, known to reduce adenylyl cyclase (AC) activity, downregulated AQP2 protein levels (15 +/- 6%) and increased urine output (220%). Downregulation of AQP2 expression in response to SR-121463A or Li treatment indicates that AQP2 expression in BB rats depends in part on activation of AVP-V(2)Rs and that the signaling cascade(s) involves AC and hence cAMP. Complete water restriction of BB rats produced only a small increase in AQP2 mRNA (235 +/- 33%) and AQP2 protein (156 +/- 22%) levels. Immunoelectron microscopy confirmed the increase in AQP2 abundance but revealed no change in AQP2 apical plasma membrane labeling in response to thirsting. In conclusion, the expression and phosphorylation of AQP2 in BB rats are in part dependent on AVP-V(2)R signaling, and AVP-V(2)-mediated regulation of AQP2 trafficking and expression is effectively decoupled in BB rats, indicating differences in AVP-V(2)R-mediated regulation of AQP2 trafficking and expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AQP2Humandiabetes insipidus  ISOAqp2 (Rattus norvegicus)protein:decreased expression:total kidney membrane fraction (rat)RGD 
AVPHumandiabetes insipidus  ISOAvp (Rattus norvegicus) RGD 
Aqp2Mousediabetes insipidus  ISOAqp2 (Rattus norvegicus)protein:decreased expression:total kidney membrane fraction (rat)RGD 
Aqp2Ratdiabetes insipidus  IAGP protein:decreased expression:total kidney membrane fraction (rat)RGD 
AvpRatdiabetes insipidus  IAGP  RGD 
AvpMousediabetes insipidus  ISOAvp (Rattus norvegicus) RGD 
AvpdiRatdiabetes insipidus  IAGP  RGD 
BRAT-Avpdi/BluHsdRatdiabetes insipidus MODEL: spontaneousIAGP  RGD 
AQP2HumanPolyuria  ISOAqp2 (Rattus norvegicus)protein:decreased expression:total kidney membrane fraction (rat)RGD 
AVPHumanPolyuria  ISOAvp (Rattus norvegicus) RGD 
Aqp2RatPolyuria  IAGP protein:decreased expression:total kidney membrane fraction (rat)RGD 
Aqp2MousePolyuria  ISOAqp2 (Rattus norvegicus)protein:decreased expression:total kidney membrane fraction (rat)RGD 
AvpRatPolyuria  IAGP  RGD 
AvpMousePolyuria  ISOAvp (Rattus norvegicus) RGD 
AvpdiRatPolyuria  IAGP  RGD 
BRAT-Avpdi/BluHsdRatPolyuria  IAGP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AvpRatpolyuria  IAGP  RGD 
AvpdiRatpolyuria  IAGP  RGD 
BRAT-Avpdi/BluHsdRatpolyuria  IAGP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Aqp2  (aquaporin 2)
Avp  (arginine vasopressin)
Avpdi  (arginine vasopressin; diabetes insipidus mutant)

Genes (Mus musculus)
Aqp2  (aquaporin 2)
Avp  (arginine vasopressin)

Genes (Homo sapiens)
AQP2  (aquaporin 2)
AVP  (arginine vasopressin)

Strains
BRAT-Avpdi/BluHsd  (Brattleboro)


Additional Information