RGD Reference Report - 5-Lipoxygenase and 5-lipoxygenase activating protein (FLAP) immunoreactivity in lungs from patients with primary pulmonary hypertension. - Rat Genome Database

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5-Lipoxygenase and 5-lipoxygenase activating protein (FLAP) immunoreactivity in lungs from patients with primary pulmonary hypertension.

Authors: Wright, L  Tuder, RM  Wang, J  Cool, CD  Lepley, RA  Voelkel, NF 
Citation: Wright L, etal., Am J Respir Crit Care Med. 1998 Jan;157(1):219-29.
RGD ID: 1626154
Pubmed: PMID:9445303   (View Abstract at PubMed)
DOI: DOI:10.1164/ajrccm.157.1.9704003   (Journal Full-text)

Inflammatory infiltrates and endothelial cell proliferation have been appreciated in plexiform and concentric lesions, which characterize the vascular remodeling in primary pulmonary hypertension (PPH). Leukotriene production by perivascular and alveolar macrophages relies on activation of 5-lipoxygenase (5-LO), with translocation of the enzyme to the nuclear membrane, and association with the 5-LO activating protein (FLAP). Using immunohistochemical staining, we localized and semi-quantitatively estimated the abundance of 5-LO and FLAP in lungs obtained from patients with PPH, patients with interstitial lung disease (ILD), and normal control subjects. Expression of 5-LO and FLAP was prominent in alveolar macrophages in both the normal and PPH lungs; however, alveolar macrophages were more frequently clustered in the vicinity of remodeled blood vessel in PPH. Medium- and small-size pulmonary arteries in PPH showed more abundant FLAP expression than in control and ILD lungs. 5-LO expression in small arteries in PPH was more intense than in control and ILD patients. Endothelial cells in plexiform and concentric lesions in PPH expressed both 5-LO and FLAP. In situ hybridization confirmed the presence of 5-LO transcripts in macrophages and endothelial cells of the remodeled vessels in PPH. We propose that the overexpression of 5-LO and FLAP represents evidence for the participation of inflammation in the process of PPH vasculopathy or, alternatively, that the overabundance of the enzymes involved in generation of inflammatory mediators may themselves be related to vascular cell proliferation and cell growth.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ALOX5Humanpulmonary hypertension  IEP protein:increased expression:arteryRGD 
ALOX5APHumanpulmonary hypertension  IEP protein:increased expression:arteryRGD 
Alox5Ratpulmonary hypertension  ISOALOX5 (Homo sapiens)protein:increased expression:arteryRGD 
Alox5Mousepulmonary hypertension  ISOALOX5 (Homo sapiens)protein:increased expression:arteryRGD 
Alox5apRatpulmonary hypertension  ISOALOX5AP (Homo sapiens)protein:increased expression:arteryRGD 
Alox5apMousepulmonary hypertension  ISOALOX5AP (Homo sapiens)protein:increased expression:arteryRGD 

Objects Annotated

Genes (Rattus norvegicus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Mus musculus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Genes (Homo sapiens)
ALOX5  (arachidonate 5-lipoxygenase)
ALOX5AP  (arachidonate 5-lipoxygenase activating protein)


Additional Information