Effect of apocynin treatment on renal expression of COX-2, NOS1, and renin in Wistar-Kyoto and spontaneously hypertensive rats. |
| Authors: |
Paliege, A Parsumathy, A Mizel, D Yang, T Schnermann, J Bachmann, S
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| Citation: |
Paliege A, etal., Am J Physiol Regul Integr Comp Physiol. 2006 Mar;290(3):R694-700. |
| RGD ID: |
1580671 |
| Pubmed: |
PMID:16467505 (View Abstract at PubMed) |
| DOI: |
DOI:10.1152/ajpregu.00219.2005 (Journal Full-text) |
Macula densa (MD) cells of the juxtaglomerular apparatus (JGA) synthesize type 1 nitric oxide synthase (NOS1) and type 2 cyclooxygenase (COX-2). Both nitric oxide (NO) and prostaglandins have been considered to mediate or modulate the control of renin secretion. Reactive oxygen species (ROS) produced locally by NADPH oxidase may influence NO bioavailability. We have tested the hypothesis that in hypertension elevated ROS levels may modify the expression of NOS1 and COX-2 in the JGA, thereby interacting with juxtaglomerular signaling. To this end, spontaneously hypertensive rats (SHR) and Wistar-Kyoto control rats (WKY) received the specific NADPH oxidase inhibitor, apocynin, during 3 wk. Renal functional and histochemical parameters, plasma renin activity (PRA), and as a measure of ROS activity, urinary isoprostane excretion (IP) were evaluated. Compared with WKY, IP levels in untreated SHR were 2.2-fold increased, and NOS1 immunoreactiviy (IR) of JGA 1.5-fold increased, whereas COX-2 IR was reduced to 35%, renin IR to 51%, and PRA to 7%. Apocynin treatment reduced IP levels in SHR to 52%, NOS1 IR to 69%, and renin IR to 62% of untreated SHR, whereas renin mRNA, COX-2 IR, glomerular filtration rate, PRA, and systolic blood pressure remained unchanged. WKY revealed no changes under apocynin treatment. These data show that NADPH oxidase is an important contributor to elevated levels of ROS in hypertension. Upregulation of MD NOS1 in SHR may have the potential of blunting the functional impact of ROS at the level of bioavailable NO. Downregulated COX-2 and renin levels in SHR are apparently unrelated to oxidative stress, since apocynin treatment had no effect on these parameters.
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| Genes (Rattus norvegicus) |
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| Ptgs2 (prostaglandin-endoperoxide synthase 2) | Ren (renin) |
| Genes (Mus musculus) |
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| Ptgs2 (prostaglandin-endoperoxide synthase 2) | Ren1 (renin 1 structural) |
| Genes (Homo sapiens) |
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| PTGS2 (prostaglandin-endoperoxide synthase 2) | REN (renin) |
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