RGD Reference Report - Effects of flavonoids on MicroRNA 145 regulation through Klf4 and myocardin in neointimal formation in vitro and in vivo. - Rat Genome Database

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Effects of flavonoids on MicroRNA 145 regulation through Klf4 and myocardin in neointimal formation in vitro and in vivo.

Authors: Lin, Chiu-Mei  Wang, Bao-Wei  Pan, Chun-Ming  Fang, Wei-Jen  Chua, Su-Kiat  Hou, Sheng-Wen  Chang, Hang  Shyu, Kou-Gi 
Citation: Lin CM, etal., J Nutr Biochem. 2018 Feb;52:27-35. doi: 10.1016/j.jnutbio.2017.08.016. Epub 2017 Sep 14.
RGD ID: 155230825
Pubmed: PMID:29127880   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jnutbio.2017.08.016   (Journal Full-text)

MicroRNA 145 (miR-145) is a critical modulator of vascular smooth muscle cell (VSMC) phenotyping and proliferation. Flavonoids have been studied extensively due to their diverse pharmacological properties, including anti-inflammatory effects. The aims of this study is designed to evaluate the atheroprotective effects on angiotensin II (Ang II)-induced miR-145 and Klf4/myocardin expression in vitro and in vivo of flavonoids, including (-)-epigallocatechin gallate (EGCG), chrysin, wogonin, silibinin, and ferulic acid. Ang II significantly reduced the miR-145 compared with the control VSMC groups; all the tested flavonoids increased miR-145 in the 100 nM concentration. Among the test compounds, EGCG showed the strongest augmenting effect on miR-145 and myocardin, however, it also abolished Ang II-induced Klf4. A [3H]-thymidine incorporation proliferation assay demonstrated that EGCG inhibited Ang II-induced VSMC proliferation, and Klf4 siRNA presented with the similar results. Immunohistochemical analysis and confocal microscopy demonstrated increased Klf4 expression and the arterial lumen was narrowed after balloon injury 14 days. With the addition of EGCG (50 mg/kg) and Klf4 siRNA, neointimal formation was reduced by 40.7% and 50.5% compared with balloon injury 14 days; Klf4 expression also was attenuated. This study demonstrated EGCG increased miR-145 and attenuated Klf4, and ameliorated neointimal formation in vitro and in vivo. The novel suppressive effect was mediated through the miR-145 and Klf4/myocardin pathways.




  
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Original Reference(s)
KLF4HumanCarotid Artery Injuries amelioratesISOKlf4 (Rattus norvegicus) RGD 
Klf4MouseCarotid Artery Injuries amelioratesISOKlf4 (Rattus norvegicus) RGD 
Klf4RatCarotid Artery Injuries amelioratesIMP  RGD 

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Object Symbol
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Original Reference(s)
KLF4Human(-)-epigallocatechin 3-gallate multiple interactionsEXP (-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II increases expression of KLF4 protein in vascular smooth muscle cells] RGD 
Klf4Mouse(-)-epigallocatechin 3-gallate multiple interactionsISOKLF4 (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II increases expression of KLF4 protein in vascular smooth muscle cells] RGD 
Klf4Rat(-)-epigallocatechin 3-gallate multiple interactionsISOKLF4 (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II increases expression of KLF4 protein in vascular smooth muscle cells] RGD 
MIR145Human(-)-epigallocatechin 3-gallate multiple interactionsEXP (-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cell] RGD 
MYOCDHuman(-)-epigallocatechin 3-gallate multiple interactionsEXP (-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MYOCD protein in vascular smooth muscle cell]RGD 
Mir145Rat(-)-epigallocatechin 3-gallate multiple interactionsISOMIR145 (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cell] RGD 
Mir145aMouse(-)-epigallocatechin 3-gallate multiple interactionsISOMIR145 (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cell] RGD 
MyocdRat(-)-epigallocatechin 3-gallate multiple interactionsISOMYOCD (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MYOCD protein in vascular smooth muscle cell]RGD 
MyocdMouse(-)-epigallocatechin 3-gallate multiple interactionsISOMYOCD (Homo sapiens)(-)-epigallocatechin 3-gallate inhibits the reaction [angiotensin II decreases expression of MYOCD protein in vascular smooth muscle cell]RGD 
KLF4Humanangiotensin II increases expression EXP angiotensin II increases expression of KLF4 mRNA and protein in vascular smooth muscle cells RGD 
Klf4Mouseangiotensin II increases expression ISOKLF4 (Homo sapiens)angiotensin II increases expression of KLF4 mRNA and protein in vascular smooth muscle cells RGD 
Klf4Ratangiotensin II increases expression ISOKLF4 (Homo sapiens)angiotensin II increases expression of KLF4 mRNA and protein in vascular smooth muscle cells RGD 
MIR145Humanangiotensin II decreases expression EXP angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cellRGD 
MYOCDHumanangiotensin II decreases expression EXP angiotensin II decreases expression of MYOCD mRNA and protein in vascular smooth muscle cellRGD 
Mir145Ratangiotensin II decreases expression ISOMIR145 (Homo sapiens)angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cellRGD 
Mir145aMouseangiotensin II decreases expression ISOMIR145 (Homo sapiens)angiotensin II decreases expression of MIR145a RNA in vascular smooth muscle cellRGD 
MyocdRatangiotensin II decreases expression ISOMYOCD (Homo sapiens)angiotensin II decreases expression of MYOCD mRNA and protein in vascular smooth muscle cellRGD 
MyocdMouseangiotensin II decreases expression ISOMYOCD (Homo sapiens)angiotensin II decreases expression of MYOCD mRNA and protein in vascular smooth muscle cellRGD 
KLF4Humanchrysin multiple interactionsEXP chrysin inhibits the reaction [angiotensin II increases expression of KLF4 protein in vascular smooth muscle cells] RGD 
Klf4Mousechrysin multiple interactionsISOKLF4 (Homo sapiens)chrysin inhibits the reaction [angiotensin II increases expression of KLF4 protein in vascular smooth muscle cells] RGD 

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Genes (Rattus norvegicus)
Klf4  (KLF transcription factor 4) Mir145  (microRNA 145) Myocd  (myocardin)

Genes (Mus musculus)
Klf4  (Kruppel-like transcription factor 4 (gut)) Mir145a  (microRNA 145a) Myocd  (myocardin)

Genes (Homo sapiens)
KLF4  (KLF transcription factor 4) MIR145  (microRNA 145) MYOCD  (myocardin)