RGD Reference Report - Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1. - Rat Genome Database

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Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1.

Authors: Lichanska, AM  Browne, CM  Henkel, GW  Murphy, KM  Ostrowski, MC  McKercher, SR  Maki, RA  Hume, DA 
Citation: Lichanska AM, etal., Blood 1999 Jul 1;94(1):127-38.
RGD ID: 1549495
Pubmed: PMID:10381505   (View Abstract at PubMed)

During mouse embryogenesis, macrophage-like cells arise first in the yolk sac and are produced subsequently in the liver. The onset of liver hematopoiesis is associated with the transition from primitive to definitive erythrocyte production. This report addresses the hypothesis that a similar transition in phenotype occurs in myelopoiesis. We have used whole mount in situ hybridization to detect macrophage-specific genes expressed during mouse development. The mouse c-fms mRNA, encoding the receptor for macrophage colony-stimulating factor (CSF-1), was expressed on phagocytic cells in the yolk sac and throughout the embryo before the onset of liver hematopoiesis. Similar cells were detected using the mannose receptor, the complement receptor (CR3), or the Microphthalmia transcription factor (MITF) as mRNA markers. By contrast, other markers including the F4/80 antigen, the macrophage scavenger receptor, the S-100 proteins, S100A8 and S100A9, and the secretory product lysozyme appeared later in development and appeared restricted to only a subset of c-fms-positive cells. Two-color immunolabeling on disaggregated cells confirmed that CR3 and c-fms proteins are expressed on the same cells. Among the genes appearing later in development was the macrophage-restricted transcription factor, PU.1, which has been shown to be required for normal adult myelopoiesis. Mice with null mutations in PU.1 had normal numbers of c-fms-positive phagocytes at 11.5dpc. PU.1(-/-) embryonic stem cells were able to give rise to macrophage-like cells after cultivation in vitro. The results support previous evidence that yolk sac-derived fetal phagocytes are functionally distinct from those arising in the liver and develop via a different pathway.

Objects referenced in this article
Gene Adgre1 adhesion G protein-coupled receptor E1 Mus musculus
Gene Lyz2 lysozyme 2 Mus musculus
Gene Mrc1 mannose receptor, C type 1 Mus musculus
Gene Msr1 macrophage scavenger receptor 1 Mus musculus
Gene S100a8 S100 calcium binding protein A8 (calgranulin A) Mus musculus
Gene S100a9 S100 calcium binding protein A9 (calgranulin B) Mus musculus
Gene Spi1 Spi-1 proto-oncogene Mus musculus

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