RGD Reference Report - A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. - Rat Genome Database

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A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats.

Authors: Mansouri, MT  Hemmati, AA  Naghizadeh, B  Mard, SA  Rezaie, A  Ghorbanzadeh, B 
Citation: Mansouri MT, etal., Indian J Pharmacol. 2015 May-Jun;47(3):292-8. doi: 10.4103/0253-7613.157127.
RGD ID: 11059519
Pubmed: PMID:26069367   (View Abstract at PubMed)
PMCID: PMC4450555   (View Article at PubMed Central)
DOI: DOI:10.4103/0253-7613.157127   (Journal Full-text)

OBJECTIVES: Ellagic acid (EA) has shown antinociceptive and anti-inflammatory effects. Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes and also cytokines play a key role in many inflammatory conditions. This study was aimed to investigate the mechanisms involved in the anti-inflammatory effect of EA. MATERIALS AND METHODS: Carrageenan-induced mouse paw edema model was used for induction of inflammation. RESULTS: The results showed that intraplantar injection of carrageenan led to time-dependent development of peripheral inflammation, which resulted in a significant increase in the levels of tumor necrosis factor alpha (TNF-alpha) and interleukin 1 (IL-1) beta, nitric oxide (NO) and prostaglandin E2 (PGE2) and also iNOS and COX-2 protein expression in inflamed paw. However, systemic administration of EA (1-30 mg/kg, intraperitoneal [i.p.]) could reduce edema in a dose-dependent fashion in inflamed rat paws with ED50 value 8.41 (5.26-14.76) mg/kg. It decreased the serum concentration of NO, PGE2, aspartate aminotransferase and alanine aminotransferase, and suppress the protein expression of iNOS, COX-2 enzymes, and attenuated the formation of PGE2, TNF-alpha and IL-1 beta in inflamed paw tissue. We also demonstrated that EA significantly decreased the malondialdehyde (MDA) level in liver at 5 h after carrageenan injection. Moreover, histopathological studies indicated that EA significantly diminished migration of polymorphonuclear leukocytes into site of inflammation, as did indomethacin. CONCLUSIONS: Collectively, the anti-inflammatory mechanisms of EA might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via the suppression of pro-inflammatory cytokines (TNFalpha, IL1 beta), NO and PGE2 overproduction.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
IL1BHumanInflammation treatmentISOIl1b (Rattus norvegicus) RGD 
Il1bRatInflammation treatmentIEP  RGD 
Il1bMouseInflammation treatmentISOIl1b (Rattus norvegicus) RGD 
TNFHumanInflammation treatmentISOTnf (Rattus norvegicus) RGD 
TnfRatInflammation treatmentIEP  RGD 
TnfMouseInflammation treatmentISOTnf (Rattus norvegicus) RGD 


Genes (Rattus norvegicus)
Il1b  (interleukin 1 beta) Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Il1b  (interleukin 1 beta) Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
IL1B  (interleukin 1 beta) TNF  (tumor necrosis factor)