RGD Reference Report - RGMA and neogenin protein expression are influenced by lens injury following optic nerve crush in the rat retina. - Rat Genome Database

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RGMA and neogenin protein expression are influenced by lens injury following optic nerve crush in the rat retina.

Authors: Schnichels, S  Heiduschka, P  Julien, S 
Citation: Schnichels S, etal., Graefes Arch Clin Exp Ophthalmol. 2012 Jan;250(1):39-50. doi: 10.1007/s00417-011-1791-9. Epub 2011 Sep 2.
RGD ID: 9850142
Pubmed: PMID:21887516   (View Abstract at PubMed)
DOI: DOI:10.1007/s00417-011-1791-9   (Journal Full-text)

BACKGROUND: The death and the failure of neurons to regenerate their axons after lesion of the central nervous system in mammals, as in the case of spinal cord injury and optic nerve trauma, remain a challenge. In this study, we focused on the repulsive guidance molecule A (RGMA) and its receptor neogenin. Since it was reported that RGMA+ cells accumulate in lesioned areas after spinal cord injury, brain trauma, and optic nerve crush, and curiously, anti-apoptotic effects of RGMA were also described, we investigated the role of RGMA and neogenin in the retina after optic nerve crush (ONC). METHODS: We evaluated the spatial and temporal protein pattern of RGMA and neogenin in the rat retina without (non-regenerating model) or with (regenerating model) lens injury (LI). We investigated the presence of RGMA, neogenin and other proteins at up to nine time points (6 h-20 days post-surgery) by performing immunohistochemistry and Western blots. RESULTS: Independent of the treatment, RGMA protein was present in the nuclear layers (NLs), plexiform layers (PLs), nerve fiber layer (NFL), and in retinal ganglion cells (RGCs) of the rat retina. RGC and nerve fibers were always RGMA+. Further RGMA+ cells in the retina were blood vessel endothelial cells, astrocytes, Muller cells, and some microglial cells. The RGMA pattern for the specific retinal cells resembled those of previously published data. The neogenin pattern was congruent to the RGMA pattern. Western blots of retinal tissue showed further RGMA+ products only in LI animals. Furthermore, a higher amount of RGMA was found in the retinae of ONC + LI rats compared to ONC rats. CONCLUSIONS: Although a difference in the localization of RGMA is not obvious, the difference in the amount of RGMA is striking, the higher amount of RGMA in the retinae of ONC + LI rats compared to ONC rats indicates a role for RGMA during degeneration/regeneration processes. Our results are consistent with several reported neuroprotective effects of RGMA. Our new data showing the upregulation of RGMA after ONC in our regenerating model (plus LI) confirm these findings conducted in different settings.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NEO1HumanOptic Nerve Injuries  ISONeo1 (Rattus norvegicus)protein:increased expression:retinaRGD 
Neo1RatOptic Nerve Injuries  IEP protein:increased expression:retinaRGD 
Neo1MouseOptic Nerve Injuries  ISONeo1 (Rattus norvegicus)protein:increased expression:retinaRGD 
NEO1HumanPenetrating Eye Injuries  ISONeo1 (Rattus norvegicus)protein:increased expression:retinaRGD 
Neo1RatPenetrating Eye Injuries  IEP protein:increased expression:retinaRGD 
Neo1MousePenetrating Eye Injuries  ISONeo1 (Rattus norvegicus)protein:increased expression:retinaRGD 
RGMAHumanPenetrating Eye Injuries  ISORgma (Rattus norvegicus)protein:increased expression:retinaRGD 
RgmaRatPenetrating Eye Injuries  IEP protein:increased expression:retinaRGD 
RgmaMousePenetrating Eye Injuries  ISORgma (Rattus norvegicus)protein:increased expression:retinaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Neo1  (neogenin 1)
Rgma  (repulsive guidance molecule BMP co-receptor a)

Genes (Mus musculus)
Neo1  (neogenin)
Rgma  (repulsive guidance molecule family member A)

Genes (Homo sapiens)
NEO1  (neogenin 1)
RGMA  (repulsive guidance molecule BMP co-receptor a)

Additional Information