RGD Reference Report - Identification of a common receptor for three types of rat cytokine-induced neutrophil chemoattractants (CINCs). - Rat Genome Database

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Identification of a common receptor for three types of rat cytokine-induced neutrophil chemoattractants (CINCs).

Authors: Shibata, F  Konishi, K  Nakagawa, H 
Citation: Shibata F, etal., Cytokine. 2000 Sep;12(9):1368-73.
RGD ID: 5135014
Pubmed: PMID:10975996   (View Abstract at PubMed)
DOI: DOI:10.1006/cyto.2000.0739   (Journal Full-text)

Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1), CINC-2 and CINC-3/macrophage inflammatory protein-2 (MIP-2), members of the CXC chemokine family, are potent chemotactic factors for neutrophils. In order to identify the receptor for CINCs, rat CXC chemokine receptor 2 (CXCR2) was cloned and expressed in HEK293 cells. CINC-1, CINC-2 and CINC-3 induced calcium mobilizations dose-dependently in CXCR2-transfected cells, whereas formyl-methionyl-leucyl-phenylalanine (FMLP) did not. CINC-3 induced enhancement of cytoplasmic calcium concentration more potently than CINC-1 and CINC-2, and desensitized calcium transients induced by CINC-1 and CINC-2, which were essentially identical to those observed in rat neutrophils. In addition, anti-CXCR2 serum inhibited neutrophil chemotactic activities of three types of CINCs almost completely. The mutant CINC-3, whose amino-terminal amino acid sequence (SELR) was replaced to AAR, lost chemotactic activity of its own but inhibited that of CINC-1 and CINC-2 potently, and that of CINC-3 weakly. The results indicate that rat CXCR2 on neutrophils is the unique receptor for all three types of CINCs, and CINC-1/-2 and CINC-3 exert different biological activities through the common receptor.




Biological Process

  

Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cxcl1Ratchemokine activity  IDA  RGD 
Cxcl2Ratchemokine activity  IDA  RGD 
Cxcl3Ratchemokine activity  IDA  RGD 


Genes (Rattus norvegicus)
Cxcl1  (C-X-C motif chemokine ligand 1) Cxcl2  (C-X-C motif chemokine ligand 2) Cxcl3  (C-X-C motif chemokine ligand 3)
Cxcr2  (C-X-C motif chemokine receptor 2)