RGD Reference Report - Identification of a common receptor for three types of rat cytokine-induced neutrophil chemoattractants (CINCs). - Rat Genome Database

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Identification of a common receptor for three types of rat cytokine-induced neutrophil chemoattractants (CINCs).

Authors: Shibata, F  Konishi, K  Nakagawa, H 
Citation: Shibata F, etal., Cytokine. 2000 Sep;12(9):1368-73.
RGD ID: 5135014
Pubmed: PMID:10975996   (View Abstract at PubMed)
DOI: DOI:10.1006/cyto.2000.0739   (Journal Full-text)

Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1), CINC-2 and CINC-3/macrophage inflammatory protein-2 (MIP-2), members of the CXC chemokine family, are potent chemotactic factors for neutrophils. In order to identify the receptor for CINCs, rat CXC chemokine receptor 2 (CXCR2) was cloned and expressed in HEK293 cells. CINC-1, CINC-2 and CINC-3 induced calcium mobilizations dose-dependently in CXCR2-transfected cells, whereas formyl-methionyl-leucyl-phenylalanine (FMLP) did not. CINC-3 induced enhancement of cytoplasmic calcium concentration more potently than CINC-1 and CINC-2, and desensitized calcium transients induced by CINC-1 and CINC-2, which were essentially identical to those observed in rat neutrophils. In addition, anti-CXCR2 serum inhibited neutrophil chemotactic activities of three types of CINCs almost completely. The mutant CINC-3, whose amino-terminal amino acid sequence (SELR) was replaced to AAR, lost chemotactic activity of its own but inhibited that of CINC-1 and CINC-2 potently, and that of CINC-3 weakly. The results indicate that rat CXCR2 on neutrophils is the unique receptor for all three types of CINCs, and CINC-1/-2 and CINC-3 exert different biological activities through the common receptor.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cxcl1Ratchemokine activity  IDA  RGD 
Cxcl2Ratchemokine activity  IDA  RGD 
Cxcl3Ratchemokine activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl2  (C-X-C motif chemokine ligand 2)
Cxcl3  (C-X-C motif chemokine ligand 3)
Cxcr2  (C-X-C motif chemokine receptor 2)


Additional Information