RGD Reference Report - Involvement of acidic fibroblast growth factor in spinal cord injury repair processes revealed by a proteomics approach. - Rat Genome Database

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Involvement of acidic fibroblast growth factor in spinal cord injury repair processes revealed by a proteomics approach.

Authors: Tsai, MC  Shen, LF  Kuo, HS  Cheng, H  Chak, KF 
Citation: Tsai MC, etal., Mol Cell Proteomics. 2008 Sep;7(9):1668-87. Epub 2008 May 14.
RGD ID: 2317692
Pubmed: PMID:18482974   (View Abstract at PubMed)
PMCID: PMC2556019   (View Article at PubMed Central)
DOI: DOI:10.1074/mcp.M800076-MCP200   (Journal Full-text)

Acidic fibroblast growth factor (aFGF; also known as FGF-1) is a potent neurotrophic factor that affects neuronal survival in the injured spinal cord. However, the pathological changes that occur with spinal cord injury (SCI) and the attribution to aFGF of a neuroprotective effect during SCI are still elusive. In this study, we demonstrated that rat SCI, when treated with aFGF, showed significant functional recovery as indicated by the Basso, Beattie, and Bresnahan locomotor rating scale and the combined behavior score (p < 0.01-0.001). Furthermore proteomics and bioinformatics approaches were adapted to investigate changes in the global protein profile of the damaged spinal cord tissue when experimental rats were treated either with or without aFGF at 24 h after injury. We found that 51 protein spots, resolvable by two-dimensional PAGE, had significant differential expression. Using hierarchical clustering analysis, these proteins were categorized into five major expression patterns. Noticeably proteins involved in the process of secondary injury, such as astrocyte activation (glial fibrillary acidic protein), inflammation (S100B), and scar formation (keratan sulfate proteoglycan lumican), which lead to the blocking of injured spinal cord regeneration, were down-regulated in the contusive spinal cord after treatment with aFGF. We propose that aFGF might initiate a series of biological processes to prevent or attenuate secondary injury and that this, in turn, leads to an improvement in functional recovery. Moreover the quantitative expression level of these proteins was verified by quantitative real time PCR. Furthermore we identified various potential neuroprotective protein factors that are induced by aFGF and may be involved in the spinal cord repair processes of SCI rats. Thus, our results could have a remarkable impact on clinical developments in the area of spinal cord injury therapy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Injuries  IDA 2317692Human FGF1 used in rat model of spinal cord injuryRGD 
Spinal Cord Injuries  ISOFGF1 (Homo sapiens)2317692; 2317692Human FGF1 used in rat model of spinal cord injuryRGD 
Spinal Cord Injuries  ISOLum (Rattus norvegicus)2317692; 2317692protein:increased expression:spinal cordRGD 
Spinal Cord Injuries  IEP 2317692protein:increased expression:spinal cordRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to growth factor  IEP 2317692 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fgf1  (fibroblast growth factor 1)
Lum  (lumican)

Genes (Mus musculus)
Fgf1  (fibroblast growth factor 1)
Lum  (lumican)

Genes (Homo sapiens)
FGF1  (fibroblast growth factor 1)
LUM  (lumican)


Additional Information