RGD Reference Report - Cellular localization of type 5 and type 6 ACs in collecting duct and regulation of cAMP synthesis. - Rat Genome Database

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Cellular localization of type 5 and type 6 ACs in collecting duct and regulation of cAMP synthesis.

Authors: Helies-Toussaint, C  Aarab, L  Gasc, JM  Verbavatz, JM  Chabardes, D 
Citation: Helies-Toussaint C, etal., Am J Physiol Renal Physiol. 2000 Jul;279(1):F185-94.
RGD ID: 2315006
Pubmed: PMID:10894801   (View Abstract at PubMed)

The cellular distribution of Ca(2+)-inhibitable adenylyl cyclase (AC) type 5 and type 6 mRNAs in rat outer medullary collecting duct (OMCD) was performed by in situ hybridization. Kidney sections were also stained with specific antibodies against either collecting duct intercalated cells or principal cells. The localization of type 5 AC in H(+)-ATPase-, but not aquaporin-3-, positive cells demonstrated that type 5 AC mRNA is expressed only in intercalated cells. In contrast, type 6 AC mRNA was observed in both intercalated and principal cells. In microdissected OMCDs, the simultaneous superfusion of carbachol and PGE(2) elicited an additive increase in the intracellular Ca(2+) concentration, suggesting that the Ca(2+)-dependent regulation of these agents occurs in different cell types. Glucagon-dependent cAMP synthesis was inhibited by both a pertussis toxin-sensitive PGE(2) pathway (63.7 +/- 4.6% inhibition, n = 5) and a Ca(2+)-dependent carbachol pathway (48.6 +/- 3.3%, n = 5). The simultaneous addition of both agents induced a cumulative inhibition of glucagon-dependent cAMP synthesis (78.2 +/- 3.3%, n = 5). The results demonstrate a distinct cellular localization of type 5 and type 6 AC mRNAs in OMCD and the functional expression of these Ca(2+)-inhibitable enzymes in intercalated cells.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Adcy5RatcAMP biosynthetic process  IDA  RGD 
Adcy6RatcAMP biosynthetic process  IDA  RGD 

Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Adcy5Ratcalcium- and calmodulin-responsive adenylate cyclase activity  IDA  RGD 
Adcy6Ratcalcium- and calmodulin-responsive adenylate cyclase activity  IDA  RGD 

RGD Manual Annotations


  

Genes (Rattus norvegicus)
Adcy5  (adenylate cyclase 5) Adcy6  (adenylate cyclase 6)

Genes (Mus musculus)
Adcy5  (adenylate cyclase 5) Adcy6  (adenylate cyclase 6)

Genes (Homo sapiens)
ADCY5  (adenylate cyclase 5) ADCY6  (adenylate cyclase 6)