RGD Reference Report - Satellite cell proliferation is reduced in muscles of obese Zucker rats but restored with loading. - Rat Genome Database

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Satellite cell proliferation is reduced in muscles of obese Zucker rats but restored with loading.

Authors: Peterson, JM  Bryner, RW  Alway, SE 
Citation: Peterson JM, etal., Am J Physiol Cell Physiol. 2008 Aug;295(2):C521-8. Epub 2008 May 28.
RGD ID: 2313320
Pubmed: PMID:18508911   (View Abstract at PubMed)
PMCID: PMC2518421   (View Article at PubMed Central)
DOI: DOI:10.1152/ajpcell.00073.2008   (Journal Full-text)

The obese Zucker rat (OZR) is a model of metabolic syndrome, which has lower skeletal muscle size than the lean Zucker rat (LZR). Because satellite cells are essential for postnatal muscle growth, this study was designed to determine whether reduced satellite cell proliferation contributes to reduced skeletal mass in OZR vs. LZR. Satellite cell proliferation was determined by a constant-release 5-bromo-2-deoxyuridine (BrdU) pellet that was placed subcutaneously in each animal. Satellite cell proliferation, as determined by BrdU incorporation, was significantly attenuated in control soleus and plantaris muscles of the OZR compared with that shown in the LZR. To determine whether this attenuation of satellite cell activity could be rescued in OZR muscles, soleus and gastrocnemius muscles were denervated, placing a compensatory load on the plantaris muscle. In the LZR and the OZR after 21 days of loading, increases of approximately 25% and approximately 30%, respectively, were shown in plantaris muscle wet weight compared with that shown in the contralateral control muscle. The number of BrdU-positive nuclei increased similarly in loaded plantaris muscles from LZR and OZR. Myogenin, MyoD, and Akt protein expressions were lower in control muscles of OZR than in those of the LZR, but they were all elevated to similar levels in the loaded plantaris muscles of OZR and LZR. These data indicate that metabolic syndrome may reduce satellite cell proliferation, and this may be a factor that contributes to the reduced mass in control muscles of OZR; however, satellite cell proliferation can be restored with compensatory loading in OZR.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKT2Humanobesity  ISOAkt2 (Rattus norvegicus)protein:decreased expression:plantaris (rat)RGD 
Akt2Ratobesity  IEP protein:decreased expression:plantaris (rat)RGD 
Akt2Mouseobesity  ISOAkt2 (Rattus norvegicus)protein:decreased expression:plantaris (rat)RGD 
MYOD1Humanobesity  ISOMyod1 (Rattus norvegicus)mRNA:decreased expression:plantarisRGD 
MYOGHumanobesity  ISOMyog (Rattus norvegicus)mRNA:decreased expression:plantarisRGD 
Myod1Ratobesity  IEP mRNA:decreased expression:plantarisRGD 
Myod1Mouseobesity  ISOMyod1 (Rattus norvegicus)mRNA:decreased expression:plantarisRGD 
MyogRatobesity  IEP mRNA:decreased expression:plantarisRGD 
MyogMouseobesity  ISOMyog (Rattus norvegicus)mRNA:decreased expression:plantarisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Akt2  (AKT serine/threonine kinase 2)
Myod1  (myogenic differentiation 1)
Myog  (myogenin)

Genes (Mus musculus)
Akt2  (thymoma viral proto-oncogene 2)
Myod1  (myogenic differentiation 1)
Myog  (myogenin)

Genes (Homo sapiens)
AKT2  (AKT serine/threonine kinase 2)
MYOD1  (myogenic differentiation 1)
MYOG  (myogenin)


Additional Information