RGD Reference Report - MicroRNA-31 and MicroRNA-155 Are Overexpressed in Ulcerative Colitis and Regulate IL-13 Signaling by Targeting Interleukin 13 Receptor α-1. - Rat Genome Database

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MicroRNA-31 and MicroRNA-155 Are Overexpressed in Ulcerative Colitis and Regulate IL-13 Signaling by Targeting Interleukin 13 Receptor α-1.

Authors: Gwiggner, Markus  Martinez-Nunez, Rocio T  Whiteoak, Simon R  Bondanese, Victor P  Claridge, Andy  Collins, Jane E  Cummings, J R Fraser  Sanchez-Elsner, Tilman 
Citation: Gwiggner M, etal., Genes (Basel). 2018 Feb 13;9(2). pii: genes9020085. doi: 10.3390/genes9020085.
RGD ID: 21403682
Pubmed: PMID:29438285   (View Abstract at PubMed)
PMCID: PMC5852581   (View Article at PubMed Central)
DOI: DOI:10.3390/genes9020085   (Journal Full-text)

Interleukin-13 (IL-13) is an important Type 2 T helper (Th2) cytokine, controlling biological functions in epithelium and has been linked to asthma, atopic dermatitis and ulcerative colitis (UC). Interleukin-13 signals through IL-13 receptor α-1 (IL13RA1 (gene) and IL13Rα1 (protein)), a receptor that can be regulated by microRNAs (miRs). MicroRNAs are small non-coding single-stranded RNAs with a role in several pathologies. However, their relevance in the pathophysiology of UC, a chronic inflammatory condition of the colonic mucosa, is poorly characterised. Here, we determined the expression of IL13Rα1 in UC, its potential regulation by miRs and the subsequent effect on IL-13 signalling. Inflamed mucosa of UC patients showed decreased mRNA and protein expression of IL13RA1 when compared to healthy controls. We show that miR-31 and miR-155 are upregulated in inflamed UC mucosa and that both directly target the 3' untranslated region of IL13RA1 mRNA. Transfection of miR-31 and miR-155 mimics reduced the expression of IL13RA1 mRNA and protein, and blocked IL-13-dependent phosphorylation of signal transducer and activator of transcription 6 (STAT6) in HT-29 cells, a gut epithelium cell line. Interleukin-13 activation of suppressor of cytokine signaling 1 (SOCS1) and eotaxin-3 (CCL26) expression was also diminished. MicroRNA-31/microRNA-155 mimics also downregulated IL13RA1 in ex vivo human inflamed UC biopsies. We propose that miR-31 and miR-155 have an important role in limiting IL-13 signalling in UC disease.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ulcerative colitis  IEP 21403682; 21403682miRNA:increased expression:colonRGD 
ulcerative colitis  ISOMIR155 (Homo sapiens)21403682; 21403682miRNA:increased expression:colonRGD 
ulcerative colitis  ISOMIR31 (Homo sapiens)21403682; 21403682miRNA:increased expression:colonRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir155  (microRNA 155)
Mir31  (microRNA 31)

Genes (Mus musculus)
Mir155  (microRNA 155)
Mir31  (microRNA 31)

Genes (Homo sapiens)
MIR155  (microRNA 155)
MIR31  (microRNA 31)


Additional Information