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Presynaptic control of striatal glutamatergic neurotransmission by adenosine A1-A2A receptor heteromers.

Authors: Ciruela, F  Casado, V  Rodrigues, RJ  Lujan, R  Burgueno, J  Canals, M  Borycz, J  Rebola, N  Goldberg, SR  Mallol, J  Cortes, A  Canela, EI  Lopez-Gimenez, JF  Milligan, G  Lluis, C  Cunha, RA  Ferre, S  Franco, R 
Citation: Ciruela F, etal., J Neurosci. 2006 Feb 15;26(7):2080-7.
Pubmed: (View Article at PubMed) PMID:16481441
DOI: Full-text: DOI:10.1523/JNEUROSCI.3574-05.2006

The functional role of heteromers of G-protein-coupled receptors is a matter of debate. In the present study, we demonstrate that heteromerization of adenosine A1 receptors (A1Rs) and A2A receptors (A2ARs) allows adenosine to exert a fine-tuning modulation of glutamatergic neurotransmission. By means of coimmunoprecipitation, bioluminescence and time-resolved fluorescence resonance energy transfer techniques, we showed the existence of A1R-A2AR heteromers in the cell surface of cotransfected cells. Immunogold detection and coimmunoprecipitation experiments indicated that A1R and A2AR are colocalized in the same striatal glutamatergic nerve terminals. Radioligand-binding experiments in cotransfected cells and rat striatum showed that a main biochemical characteristic of the A1R-A2AR heteromer is the ability of A2AR activation to reduce the affinity of the A1R for agonists. This provides a switch mechanism by which low and high concentrations of adenosine inhibit and stimulate, respectively, glutamate release. Furthermore, it is also shown that A1R-A2AR heteromers constitute a unique target for caffeine and that chronic caffeine treatment leads to modifications in the function of the A1R-A2AR heteromer that could underlie the strong tolerance to the psychomotor effects of caffeine.


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RGD Object Information
RGD ID: 1625231
Created: 2007-05-30
Species: All species
Last Modified: 2007-05-30
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.