RGD Reference Report - MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury.

Authors: Zheng, Gui-Hong  Wen, Xin  Wang, Yong-Jian  Han, Xin-Rui  Shan, Qun  Li, Wang  Zhao, Tian  Wu, Dong-Mei  Lu, Jun  Zheng, Yuan-Lin 
Citation: Zheng GH, etal., J Cell Biochem. 2018 Apr;119(4):3149-3161. doi: 10.1002/jcb.26466. Epub 2018 Jan 2.
RGD ID: 13825150
Pubmed: PMID:29073721   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.26466   (Journal Full-text)

This study aims to explore whether microRNA-381 (miR-381) mediating CXCR4 affects the renal tubular epithelial cells (RTEC) of renal ischemia reperfusion (I/R) injury. Forty-eight rats were assigned into the I/R (n¿=¿24, successfully established as I/R model) and sham (n¿=¿24) groups. After collecting kidney tissues, immunohistochemistry, and microvascular density (MVD) counting were conducted for CXCR4 positive expression and MVD numbers. RTECs were assigned into the sham, blank, negative control (NC), miR-381 mimics, miR-381 inhibitor, si-CXCR4, and miR-381 inhibitor¿+¿si-CXCR4 groups. RT-qPCR and Western blotting were performed for relative expressions in tissues and cells. Cell proliferation and apoptosis were measured by MTT assay and flow cytometry. Results showed that compared with the sham group, positive expression of CXCR4 and MVD number were higher in the I/R group, which exhibited decreased miR-381 and increased expression of CXCR4, stromal cell-derived factor-1 (SDF1), vascular endothelial growth factor (VEGF), hypoxia-inducible factor 1 (HIF-1α) and Tie-2. Dual luciferase reporter gene assay verified that CXCR4 is a target gene of miR-381. MiR-381 expression was lower in the miR-381 inhibitor¿+¿si-CXCR4 and miR-381 inhibitor groups and higher in the miR-381 mimics group than the blank and NC groups. Compared with the blank and NC groups, the miR-381 mimics and si-CXCR4 groups exhibited higher cell proliferation but lower cell apoptosis and expression of CXCR4, SDF1, VEGF, HIF-1α, and Tie-2, whereas the miR-381 inhibitor group exhibited the opposite trend. In conclusion, miR-381 may promote RTEC proliferation in rats with renal I/R injury by down-regulating CXCR4.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
CXCR4HumanKidney Reperfusion Injury  ISOCxcr4 (Rattus norvegicus)protein:increased expression:kidneyRGD 
Cxcr4RatKidney Reperfusion Injury  IEP protein:increased expression:kidneyRGD 
Cxcr4MouseKidney Reperfusion Injury  ISOCxcr4 (Rattus norvegicus)protein:increased expression:kidneyRGD 
MIR381HumanKidney Reperfusion Injury  ISOMir381 (Rattus norvegicus)RNA:decreased expression:kidneyRGD 
Mir381MouseKidney Reperfusion Injury  ISOMir381 (Rattus norvegicus)RNA:decreased expression:kidneyRGD 
Mir381RatKidney Reperfusion Injury  IEP RNA:decreased expression:kidneyRGD 


Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Mir381Ratnegative regulation of epithelial cell apoptotic process  IMP  RGD 
Mir381Ratpositive regulation of epithelial cell proliferation  IMP  RGD 


Genes (Rattus norvegicus)
Cxcr4  (C-X-C motif chemokine receptor 4) Mir381  (microRNA 381)

Genes (Mus musculus)
Cxcr4  (C-X-C motif chemokine receptor 4) Mir381  (microRNA 381)

Genes (Homo sapiens)
CXCR4  (C-X-C motif chemokine receptor 4) MIR381  (microRNA 381)