RGD Reference Report - Involvement of a7nAChR in electroacupuncture relieving neuropathic pain in the spinal cord of rat with spared nerve injury. - Rat Genome Database

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Involvement of a7nAChR in electroacupuncture relieving neuropathic pain in the spinal cord of rat with spared nerve injury.

Authors: Wang, Ying  Jiang, Qian  Xia, Yang-Yang  Huang, Zhi-Hua  Huang, Cheng 
Citation: Wang Y, etal., Brain Res Bull. 2018 Mar;137:257-264. doi: 10.1016/j.brainresbull.2018.01.002. Epub 2018 Jan 4.
RGD ID: 13792836
Pubmed: PMID:29307658   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainresbull.2018.01.002   (Journal Full-text)

Alpha-7 nicotinic acetylcholine receptor (α7nAChR) was reported to be involved in the modulation of neuropathic pain. Electroacupuncture (EA) has therapeutic effects on neuropathic pain induced by nerve injury, but the underlying mechanisms remain unclear. The present study was designed to investigate whether α7nAChR participates in the relieving effects of 2¿Hz EA on neuropathic pain. Paw withdrawal threshold (PWT) was measured to study the EA-mediated analgesic effect in a rat model of spared nerve injury (SNI). The spinal α7nAChR and IL-1ß expression levels were determined by RT-PCR, Western blot analysis, and immunofluorescence staining. Additionally, immunofluorescence targeting the expression of CD11b, which is a molecular indicator of microglial activation. The results showed that 2¿Hz EA stimulation significantly improved the expression of α7nAChR and reduced the production of IL-1ß and CD11b in the spinal cord of rats with SNI-induced neuropathic pain, along with the relief of mechanical hypersensitivity after EA treatment. Moreover, intrathecal injection of alpha-bungarotoxin (α-Bgtx), a selective antagonist for α7nAChR, at the dosage of 1.0¿µg/kg, not only suppressed the analgesic effect of EA in SNI rats, but also inhibited the enhancement of α7nAChR expression and the reduction of IL-1ß expression induced by EA. In conclusion, our study indicated that 2¿Hz EA reduces SNI-induced mechanical hypersensitivity via upregulating α7nAChR and downregulating IL-1ß and CD11b in the spinal cord of SNI rats, which might be one of the mechanisms underlying its effectiveness in the neuropathic pain.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
polyneuropathy treatmentISOIl1b (Rattus norvegicus)13792836; 13792836 RGD 
polyneuropathy treatmentIEP 13792836 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il1b  (interleukin 1 beta)

Genes (Mus musculus)
Il1b  (interleukin 1 beta)

Genes (Homo sapiens)
IL1B  (interleukin 1 beta)


Additional Information