RGD Reference Report - Accessory proteins stabilize the acceptor complex for synaptobrevin, the 1:1 syntaxin/SNAP-25 complex. - Rat Genome Database

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Accessory proteins stabilize the acceptor complex for synaptobrevin, the 1:1 syntaxin/SNAP-25 complex.

Authors: Weninger, Keith  Bowen, Mark E  Choi, Ucheor B  Chu, Steven  Brunger, Axel T 
Citation: Weninger K, etal., Structure. 2008 Feb;16(2):308-20. doi: 10.1016/j.str.2007.12.010.
RGD ID: 13432279
Pubmed: PMID:18275821   (View Abstract at PubMed)
PMCID: PMC2856644   (View Article at PubMed Central)
DOI: DOI:10.1016/j.str.2007.12.010   (Journal Full-text)

Syntaxin/SNAP-25 interactions precede assembly of the ternary SNARE complex that is essential for neurotransmitter release. This binary complex has been difficult to characterize by bulk methods because of the prevalence of a 2:1 dead-end species. Here, using single-molecule fluorescence, we find the structure of the 1:1 syntaxin/SNAP-25 binary complex is variable, with states changing on the second timescale. One state corresponds to a parallel three-helix bundle, whereas other states show one of the SNAP-25 SNARE domains dissociated. Adding synaptobrevin suppresses the dissociated helix states. Remarkably, upon addition of complexin, Munc13, Munc18, or synaptotagmin, a similar effect is observed. Thus, the 1:1 binary complex is a dynamic acceptor for synaptobrevin binding, and accessory proteins stabilize this acceptor. In the cellular environment the binary complex is actively maintained in a configuration where it can rapidly interact with synaptobrevin, so formation is not likely a limiting step for neurotransmitter release.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P3285113432279PMID:18275821IntAct 
protein binding enablesIPIUniProtKB:P60881-213432279PMID:18275821IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Snap25  (synaptosome associated protein 25)
Stx1a  (syntaxin 1A)


Additional Information