RGD Reference Report - Alterations in expression pattern of splicing factors in epithelial ovarian cancer and its clinical impact. - Rat Genome Database

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Alterations in expression pattern of splicing factors in epithelial ovarian cancer and its clinical impact.

Authors: Iborra, S  Hirschfeld, M  Jaeger, M  Zur Hausen, A  Braicu, I  Sehouli, J  Gitsch, G  Stickeler, E 
Citation: Iborra S, etal., Int J Gynecol Cancer. 2013 Jul;23(6):990-6. doi: 10.1097/IGC.0b013e31829783e3.
RGD ID: 11039469
Pubmed: PMID:23748175   (View Abstract at PubMed)
DOI: DOI:10.1097/IGC.0b013e31829783e3   (Journal Full-text)

OBJECTIVE: Alternative splicing represents an important nuclear mechanism in the posttranscriptional regulation of gene expression, which is frequently altered during tumorigenesis. Previously, we described marked changes in alternative splicing of the CD44 gene in ovarian and breast cancer as well as specific induction of distinct splicing factors during tumor development. The present study was focused on the expression profiles of different splicing factors, including classical serine-arginine (SR) proteins including ASF/SF2, hTra2beta1, hTra2alpha, and Y-box-binding protein (YB-1) in physiological and malignant epithelial ovarian tissue to evaluate their expression pattern with regard to tumor development and disease progression. MATERIALS AND METHODS: Expression levels of the different splicing factors were analyzed in physiological epithelial ovarian tissue samples, primary tumors, and metastatic samples of patients with a diagnosis of epithelial ovarian cancer using quantified reverse transcription polymerase chain reaction analysis. We examined more closely the splicing factor hTra2beta1 using Western blot analysis and immunohistochemistry. RESULTS: The analysis revealed a marked and specific induction of ASF/SF2, SRp20, hTra2beta1, and YB-1 in primary tumors as well as in their metastatic sites. However, in our patient cohort, no induction was seen for the other investigated splicing factors SRp55, SRp40, and hTra2alpha. CONCLUSIONS: Our results suggest a specific induction of distinct splicing factors in ovarian cancer tumorigenesis. The involvement of hTra2beta1, YB-1, SRp20, and ASF/SF2 in exon recognition and alternative splicing may be important for gene regulation of alternatively spliced genes like CD44 with potential functional consequences in this tumor type leading to progression and metastasis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SRSF5HumanNeoplasm Metastasis  IEP associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
SRSF6HumanNeoplasm Metastasis  IEP associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf5RatNeoplasm Metastasis  ISOSRSF5 (Homo sapiens)associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf5MouseNeoplasm Metastasis  ISOSRSF5 (Homo sapiens)associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf6RatNeoplasm Metastasis  ISOSRSF6 (Homo sapiens)associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf6MouseNeoplasm Metastasis  ISOSRSF6 (Homo sapiens)associated with Ovarian Neoplasms and mRNA:increased expression:epithelium of female gonad (human)RGD 
SRSF1HumanOvarian Neoplasms  IEP mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf1RatOvarian Neoplasms  ISOSRSF1 (Homo sapiens)mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf1MouseOvarian Neoplasms  ISOSRSF1 (Homo sapiens)mRNA:increased expression:epithelium of female gonad (human)RGD 
SRSF3Humanovary epithelial cancer  IEP mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf3Ratovary epithelial cancer  ISOSRSF3 (Homo sapiens)mRNA:increased expression:epithelium of female gonad (human)RGD 
Srsf3Mouseovary epithelial cancer  ISOSRSF3 (Homo sapiens)mRNA:increased expression:epithelium of female gonad (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Srsf1  (serine and arginine rich splicing factor 1)
Srsf3  (serine and arginine rich splicing factor 3)
Srsf5  (serine and arginine rich splicing factor 5)
Srsf6  (serine and arginine rich splicing factor 6)

Genes (Mus musculus)
Srsf1  (serine and arginine-rich splicing factor 1)
Srsf3  (serine and arginine-rich splicing factor 3)
Srsf5  (serine and arginine-rich splicing factor 5)
Srsf6  (serine and arginine-rich splicing factor 6)

Genes (Homo sapiens)
SRSF1  (serine and arginine rich splicing factor 1)
SRSF3  (serine and arginine rich splicing factor 3)
SRSF5  (serine and arginine rich splicing factor 5)
SRSF6  (serine and arginine rich splicing factor 6)


Additional Information