RGD Reference Report - Effect of plant neutrophil elastase inhibitor on leucocyte migration, adhesion and cytokine release in inflammatory conditions. - Rat Genome Database

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Effect of plant neutrophil elastase inhibitor on leucocyte migration, adhesion and cytokine release in inflammatory conditions.

Authors: Oliveira, C  Navarro-Xavier, RA  Anjos-Vallota, EA  Martins, JO  Silveira, VL  Goncalves, LR  Araujo, MS  Motta, G  Sannomiya, P  Oliva, ML 
Citation: Oliveira C, etal., Br J Pharmacol. 2010 Oct;161(4):899-910. doi: 10.1111/j.1476-5381.2010.00924.x.
RGD ID: 10450565
Pubmed: PMID:20860667   (View Abstract at PubMed)
PMCID: PMC2992903   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1476-5381.2010.00924.x   (Journal Full-text)

BACKGROUND AND PURPOSE: The serine and cysteine peptidase inhibitor, BbCI, isolated from Bauhinia bauhinioides seeds, is similar to the classical plant Kunitz inhibitor, STI, but lacks disulphide bridges and methionine residues. BbCI blocks activity of the serine peptidases, elastase (K(iapp) 5.3 nM) and cathepsin G (K(iapp) 160.0 nM), and the cysteine peptidase cathepsin L (K(iapp) 0.2 nM). These three peptidases play important roles in the inflammatory process. EXPERIMENTAL APPROACH: We measured the effects of BbCI on paw oedema and on leucocyte accumulation in pleurisy, both induced by carrageenan. Leucocyte-endothelial cell interactions in scrotal microvasculature in Wistar rats were investigated using intravital microscopy. Cytokine levels in pleural exudate and serum were measured by elisa. KEY RESULTS: Pretreatment of the animals with BbCI (2.5 mg.kg(-1)), 30 min before carrageenan-induced inflammation, effectively reduced paw oedema and bradykinin release, neutrophil migration into the pleural cavity. The number of rolling, adhered and migrated leucocytes at the spermatic fascia microcirculation following carrageenan injection into the scrotum were reduced by BbCI pretreatment. Furthermore, levels of the rat chemokine cytokine-induced neutrophil chemo-attractant-1 were significantly reduced in both pleural exudates and serum from animals pretreated with BbCI. Levels of interleukin-1beta or tumour necrosis factor-alpha, however, did not change. CONCLUSIONS AND IMPLICATIONS: Taken together, our data suggest that the anti-inflammatory properties of BbCI may be useful in investigations of other pathological processes in which human neutrophil elastase, cathepsin G and cathepsin L play important roles.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ELANEHumanEdema treatmentISOElane (Rattus norvegicus) RGD 
ElaneMouseEdema treatmentISOElane (Rattus norvegicus) RGD 
ElaneRatEdema treatmentIMP  RGD 
KNG1HumanEdema treatmentISOKng2 (Rattus norvegicus) RGD 
Kng2RatEdema treatmentIEP  RGD 
Kng2MouseEdema treatmentISOKng2 (Rattus norvegicus) RGD 
ELANEHumanpleurisy  ISOElane (Rattus norvegicus) RGD 
ElaneRatpleurisy  IMP  RGD 
ElaneMousepleurisy  ISOElane (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ElaneRatleukocyte migration involved in inflammatory response  IMP  RGD 
ElaneRatpositive regulation of leukocyte tethering or rolling  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Elane  (elastase, neutrophil expressed)
Kng2  (kininogen 2)

Genes (Mus musculus)
Elane  (elastase, neutrophil expressed)
Kng2  (kininogen 2)

Genes (Homo sapiens)
ELANE  (elastase, neutrophil expressed)
KNG1  (kininogen 1)


Additional Information