Npff (neuropeptide FF-amide peptide precursor) - Rat Genome Database

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Gene: Npff (neuropeptide FF-amide peptide precursor) Rattus norvegicus
Symbol: Npff
Name: neuropeptide FF-amide peptide precursor
RGD ID: 62033
Description: Enables G protein-coupled receptor binding activity and neuropeptide hormone activity. Involved in several processes, including negative regulation of appetite; negative regulation of insulin secretion; and peptide hormone secretion. Located in several cellular components, including axon terminus; dendrite; and perikaryon. Is active in neuronal dense core vesicle. Orthologous to human NPFF (neuropeptide FF-amide peptide precursor); INTERACTS WITH bisphenol A; endosulfan; indole-3-methanol.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: FMRFamide-related peptides; pro-FMRFamide-related neuropeptide FF
RGD Orthologs
Green Monkey
Naked Mole-Rat
Alliance Genes
More Info more info ...
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.27133,654,530 - 133,655,319 (-)NCBImRatBN7.2mRatBN7.2
UTH_Rnor_SHR_Utx7135,410,275 - 135,411,064 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.07137,639,642 - 137,640,431 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.07137,625,895 - 137,626,684 (-)NCBIRnor_WKY
Rnor_6.07144,127,084 - 144,127,873 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.07141,919,079 - 141,919,868 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
Celera7130,082,133 - 130,082,891 (-)NCBICelera
Cytogenetic Map7q36NCBI
JBrowse: View Region in Genome Browser (JBrowse)

Disease Annotations     Click to see Annotation Detail View

Gene-Chemical Interaction Annotations     Click to see Annotation Detail View

References - curated
# Reference Title Reference Citation
1. Centrally administered neuropeptide FF inhibits arginine vasopressin release in conscious rats. Arima H, etal., Endocrinology. 1996 May;137(5):1523-9.
2. Identification and characterization of two G protein-coupled receptors for neuropeptide FF. Bonini JA, etal., J Biol Chem 2000 Dec 15;275(50):39324-31.
3. Synaptic actions of neuropeptide FF in the rat parabrachial nucleus: interactions with opioid receptors. Chen X, etal., J Neurophysiol. 2000 Aug;84(2):744-51.
4. Changes in neuropeptide FF and NPY immunohistochemical patterns in rat brain under heroin treatment. D'Este L, etal., Brain Res. 2006 Apr 14;1083(1):151-8. Epub 2006 Mar 10.
5. Release of neuropeptide FF, an anti-opioid peptide, in rat spinal cord slices is voltage- and Ca(2+)-sensitive: possible involvement of P-type Ca2+ channels. Devillers JP, etal., J Neurochem. 1995 Apr;64(4):1567-75.
6. A novel cryptic peptide derived from the rat neuropeptide FF precursor reverses antinociception and conditioned place preference induced by morphine. Dylag T, etal., Peptides. 2008 Mar;29(3):473-8. Epub 2007 Sep 29.
7. The effects of two FMRFamide related peptides (A-18-F-amide and F-8-F-amide; 'morphine modulating peptides') on the endocrine and exocrine rat pancreas. Fehmann HC, etal., Neuropeptides. 1990 Oct;17(2):87-92.
8. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
9. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
10. Central administration of neuropeptide FF (NPFF) causes increased neuronal activation and up-regulation of NPFF gene expression in the rat brainstem. Jhamandas JH and Mactavish D, J Comp Neurol 2002 Jun 3;447(3):300-7.
11. Neuropeptide FF and neuropeptide VF inhibit GABAergic neurotransmission in parvocellular neurons of the rat hypothalamic paraventricular nucleus. Jhamandas JH, etal., Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1872-80. Epub 2007 Feb 8.
12. Neuropeptide FF, but not prolactin-releasing peptide, mRNA is differentially regulated in the hypothalamic and medullary neurons after salt loading. Kalliomaki ML and Panula P, Neuroscience 2004;124(1):81-7.
13. Ultrastructural localization of neuropeptide FF, a new neuropeptide in the brain and pituitary of rats. Kivipelto L Regul Pept. 1991 Jul 9;34(3):211-24.
14. Cardiovascular effects induced by the stimulation of neuropeptide FF receptors in the dorsal vagal complex: an autoradiographic and pharmacological study in the rat. Laguzzi R, etal., Brain Res. 1996 Mar 4;711(1-2):193-202.
15. Rat ISS GO annotations from MGI mouse gene data--August 2006 MGD data from the GO Consortium
16. Neuropeptide FF reduces food intake in rats. Murase T, etal., Peptides. 1996;17(2):353-4.
17. Electronic Transfer of LocusLink and RefSeq Data NCBI rat LocusLink and RefSeq merged data July 26, 2002
18. Expression of neuropeptide FF, prolactin-releasing peptide, and the receptor UHR1/GPR10 genes during embryogenesis in the rat. Nieminen ML, etal., Dev Dyn 2003 Mar;226(3):561-9.
19. Pain- and morphine-associated transcriptional regulation of neuropeptide FF and the G-protein-coupled NPFF2 receptor gene. Nystedt JM, etal., Neurobiol Dis. 2004 Jun;16(1):254-62.
20. GOA pipeline RGD automated data pipeline
21. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
22. Tentative Sequence Identification Numbers Tentative Sequence Data IDs. TIGR Gene Index, Rat Data
23. Gene for pain modulatory neuropeptide NPFF: induction in spinal cord by noxious stimuli. Vilim FS, etal., Mol Pharmacol 1999 May;55(5):804-11.
Additional References at PubMed
PMID:11587714   PMID:12668052   PMID:14557236   PMID:16517015   PMID:18295932   PMID:22342307   PMID:24316399   PMID:25247577   PMID:28825666   PMID:33255594  


Comparative Map Data
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.27133,654,530 - 133,655,319 (-)NCBImRatBN7.2mRatBN7.2
UTH_Rnor_SHR_Utx7135,410,275 - 135,411,064 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.07137,639,642 - 137,640,431 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.07137,625,895 - 137,626,684 (-)NCBIRnor_WKY
Rnor_6.07144,127,084 - 144,127,873 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.07141,919,079 - 141,919,868 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
Celera7130,082,133 - 130,082,891 (-)NCBICelera
Cytogenetic Map7q36NCBI
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
GRCh381253,506,688 - 53,507,484 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p13 Ensembl1253,506,688 - 53,507,484 (-)EnsemblGRCh38hg38GRCh38
GRCh371253,900,472 - 53,901,268 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 361252,186,741 - 52,187,689 (-)NCBINCBI36Build 36hg18NCBI36
Build 341252,186,740 - 52,187,689NCBI
Celera1253,550,832 - 53,551,780 (-)NCBICelera
Cytogenetic Map12q13.13NCBI
HuRef1250,940,056 - 50,941,004 (-)NCBIHuRef
CHM1_11253,867,530 - 53,868,478 (-)NCBICHM1_1
T2T-CHM13v2.01253,472,693 - 53,473,489 (-)NCBIT2T-CHM13v2.0
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
GRCm3915102,432,274 - 102,433,056 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl15102,432,274 - 102,433,377 (-)EnsemblGRCm39 Ensembl
GRCm3815102,523,839 - 102,524,621 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl15102,523,839 - 102,524,942 (-)EnsemblGRCm38mm10GRCm38
MGSCv3715102,354,270 - 102,355,373 (-)NCBIGRCm37MGSCv37mm9NCBIm37
MGSCv3615102,351,873 - 102,352,655 (-)NCBIMGSCv36mm8
Celera15104,681,604 - 104,682,707 (-)NCBICelera
Cytogenetic Map15F3NCBI
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
ChiLan1.0 EnsemblNW_004955458799,072 - 799,745 (-)EnsemblChiLan1.0
ChiLan1.0NW_004955458799,072 - 799,745 (-)NCBIChiLan1.0ChiLan1.0
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
PanPan1.11236,030,056 - 36,031,591 (+)NCBIpanpan1.1PanPan1.1panPan2
PanPan1.1 Ensembl1236,030,805 - 36,031,591 (+)Ensemblpanpan1.1panPan2
Mhudiblu_PPA_v01235,245,172 - 35,246,710 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
HiC_Itri_2NW_02440494562,382,476 - 62,384,553 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_00493651210,780,354 - 10,780,930 (-)EnsemblSpeTri2.0
SpeTri2.0NW_00493651210,780,277 - 10,782,219 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
Sscrofa11.1 Ensembl518,723,784 - 18,724,826 (-)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.1518,724,078 - 18,724,844 (-)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2519,159,707 - 19,160,579 (-)NCBISscrofa10.2Sscrofa10.2susScr3
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
ChlSab1.11149,607,352 - 49,608,304 (-)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 Ensembl1149,607,443 - 49,608,139 (-)EnsemblChlSab1.1ChlSab1.1 EnsemblchlSab2
Vero_WHO_p1.0NW_023666037196,445,746 - 196,446,698 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
(Heterocephalus glaber - naked mole-rat)
Naked Mole-rat AssemblyChrPosition (strand)SourceGenome Browsers
HetGla_female_1.0 EnsemblNW_0046249041,603,743 - 1,605,519 (-)EnsemblHetGla_female_1.0HetGla_female_1.0 EnsemblhetGla2
HetGla 1.0NW_0046249041,603,743 - 1,606,141 (-)NCBIHetGla_female_1.0HetGla 1.0hetGla2


Variants in Npff
2 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:87
Count of miRNA genes:76
Interacting mature miRNAs:81
Prediction methods:Microtar, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (
For more information about miRGate, see PMID:25858286 or access the full paper here.

QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1300179Kidm5Kidney mass QTL 53.51kidney mass (VT:0002707)left kidney wet weight (CMO:0000083)743747012135012528Rat
70173Niddm19Non-insulin dependent diabetes mellitus QTL 194.330.00005blood glucose amount (VT:0000188)blood glucose level area under curve (AUC) (CMO:0000350)764002457135012528Rat
1358891Bp265Blood pressure QTL 2652.21arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)783591953134666232Rat
1358914Bp266Blood pressure QTL 266arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)783591953134666232Rat
1549899Stresp8Stress response QTL 84.370.0008stress-related behavior trait (VT:0010451)defensive burying duration (CMO:0001961)790482196135012528Rat
2299163Iddm34Insulin dependent diabetes mellitus QTL 342.71blood glucose amount (VT:0000188)age at onset/diagnosis of type 1 diabetes mellitus (CMO:0001140)791281130135012528Rat
731176Glom5Glomerulus QTL 52.50.0035kidney glomerulus morphology trait (VT:0005325)count of superficial glomeruli not directly contacting the kidney surface (CMO:0001002)796670164135012528Rat
731174Uae23Urinary albumin excretion QTL 232.40.0042urine albumin amount (VT:0002871)urine albumin excretion rate (CMO:0000757)7104603555135012528Rat
2306821Bp335Blood pressure QTL 3350.001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)7106571501135012528Rat
631663Bw6Body weight QTL 63.4body mass (VT:0001259)body weight (CMO:0000012)7111075573134976056Rat
1300112Bp183Blood pressure QTL 1833.51arterial blood pressure trait (VT:2000000)blood pressure time series experimental set point of the baroreceptor response (CMO:0002593)7111182207135012528Rat
1354582Stl11Serum triglyceride level QTL 113.42blood triglyceride amount (VT:0002644)serum triglyceride level (CMO:0000360)7119513385135012528Rat

Markers in Region
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.27133,654,086 - 133,654,303 (+)MAPPERmRatBN7.2
Rnor_6.07144,126,641 - 144,126,857NCBIRnor6.0
Rnor_5.07141,918,636 - 141,918,852UniSTSRnor5.0
Celera7130,081,690 - 130,081,906UniSTS
RH 3.4 Map71051.41UniSTS
Cytogenetic Map7q36UniSTS


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
Medium 2 5 10 3 17 10
Low 1 43 57 41 14 41 8 11 64 32 24 1 8
Below cutoff


Reference Sequences
RefSeq Acc Id: ENSRNOT00000074497   ⟹   ENSRNOP00000065607
RefSeq Status:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)Ensembl
RefSeq Acc Id: NM_022586   ⟹   NP_072108
RefSeq Status: VALIDATED
Rat AssemblyChrPosition (strand)Source
mRatBN7.27133,654,530 - 133,655,319 (-)NCBI
Rnor_6.07144,127,084 - 144,127,873 (-)NCBI
Rnor_5.07141,919,079 - 141,919,868 (-)NCBI
Celera7130,082,133 - 130,082,891 (-)RGD
Protein Sequences
Protein RefSeqs NP_072108 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein AAD39828 (Get FASTA)   NCBI Sequence Viewer  
  EDL86824 (Get FASTA)   NCBI Sequence Viewer  
  EDL86825 (Get FASTA)   NCBI Sequence Viewer  
  Q9WVA9 (Get FASTA)   NCBI Sequence Viewer  
Reference Sequences
RefSeq Acc Id: NP_072108   ⟸   NM_022586
- Peptide Label: preproprotein
- UniProtKB: Q9WVA9 (UniProtKB/Swiss-Prot)
- Sequence:
RefSeq Acc Id: ENSRNOP00000065607   ⟸   ENSRNOT00000074497

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-Q9WVA9-F1-model_v2 AlphaFold Q9WVA9 1-114 view protein structure


eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

RGD ID:13695704
Promoter ID:EPDNEW_R6229
Type:single initiation site
Description:neuropeptide FF-amide peptide precursor
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database,
Experiment Methods:Single-end sequencing.
Rat AssemblyChrPosition (strand)Source
Rnor_6.07144,127,847 - 144,127,907EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:62033 AgrOrtholog
BioCyc Gene G2FUF-31931 BioCyc
Ensembl Genes ENSRNOG00000047739 Ensembl
InterPro NPFF UniProtKB/Swiss-Prot
KEGG Report rno:60337 UniProtKB/Swiss-Prot
PANTHER PTHR15044 UniProtKB/Swiss-Prot
Pfam NPFF UniProtKB/Swiss-Prot
PhenoGen Npff PhenoGen
PIRSF FMRFamid-rel_pep_precur UniProtKB/Swiss-Prot
TIGR TC222691

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2004-09-10 Npff  neuropeptide FF-amide peptide precursor    neuropeptide FF-amide peptide precursor   Name updated 1299863 APPROVED
2002-06-10 Npff  neuropeptide FF-amide peptide precursor       Name updated 70584 APPROVED

RGD Curation Notes
Note Type Note Reference
gene_regulation capable of up-regulating its own gene expression in the brainstem nucleus of the solitary tract 633355