RGD Reference Report - Cosegregation analysis of natriuretic peptide genes and blood pressure in the spontaneously hypertensive rat. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Behavioral & Substance Use Disorder Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Cosegregation analysis of natriuretic peptide genes and blood pressure in the spontaneously hypertensive rat.

Authors: Ye, P  West, MJ 
Citation: Ye P and West MJ, Clin Exp Pharmacol Physiol 2003 Dec;30(12):930-6.
RGD ID: 731210
Pubmed: PMID:14678232   (View Abstract at PubMed)

1. The natriuretic peptide precursor A (Nppa) and B (Nppb) genes are candidate genes for hypertension and cardiac hypertrophy in the spontaneously hypertensive rat (SHR). The purpose of the present study was to determine the role of the Nppa and Nppb genes in the development of hypertension in the SHR. 2. A cohort (n = 162) of F2 segregating intercross animals was established between strains of hypertensive SHR and normotensive Wistar-Kyoto rats. Blood pressure and heart weight were measured in each rat at 12-16 weeks of age. Rats were genotyped using 11 informative microsatellite markers, distributed in the vicinity of the Nppa marker on rat chromosome 5 including an Nppb marker. The phenotype values were compared with genotype using the computer package mapmaker 3.0 (Whitehead Institute, Boston, MA, USA) to determine whether there was a link between the genetic variants of the natriuretic peptide family and blood pressure or cardiac hypertrophy. 3. A strong correlation was observed between the Nppa marker and blood pressure. A quantitative trait locus (QTL) for blood pressure on chromosome 5 was identified between the Nppa locus and the D5Mgh15 marker, less than 2 cM from the Nppa locus. The linkage score for the blood pressure QTL on chromosome 5 was 3.8 and the QTL accounted for 43% of the total variance of systolic blood pressure, 54% of diastolic blood pressure and 59% of mean blood pressure. No association was found between the Nppb gene and blood pressure. This is the first report of linkage between the Nppa marker and blood pressure in the rat. There was no correlation between the Nppa or Nppb genes or other markers in this region and either heart weight or left ventricular weight in F2 rats. 4. These findings suggest the existence of a blood pressure-dependent Nppa marker variant or a gene close to Nppa predisposing to spontaneous hypertension in the rat. It provides a strong foundation for further detailed genetic studies in congenic strains, which may help to narrow down the location of this gene and lead to positional cloning.



Disease Annotations    Click to see Annotation Detail View
RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Bp155Rathypertension  IAGP  RGD 
SHR/NRathypertension MODEL: spontaneousIAGP compared to WKY/N control strainRGD 
WKY/NRathypertension MODEL: controlIAGP compared to SHR/N strainRGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SHR/NRatincreased body weight  IAGP compared to WKY/N control strainRGD 
SHR/NRatincreased heart left ventricle weight  IAGP compared to WKY/N control strainRGD 
SHR/NRatincreased mean systemic arterial blood pressure  IAGP compared to WKY/N control strainRGD 
Bp155Ratincreased systemic arterial blood pressure  IAGP  RGD 
SHR/NRatincreased systemic arterial diastolic blood pressure  IAGP compared to WKY/N control strainRGD 
SHR/NRatincreased systemic arterial systolic blood pressure  IAGP compared to WKY/N control strainRGD 

Objects Annotated

QTLs
Bp155  (Blood pressure QTL 155)

Strains
SHR/N  (NA)
WKY/N  (NA)

Objects referenced in this article
Gene NPPA natriuretic peptide A Homo sapiens
Gene Nppa natriuretic peptide type A Mus musculus
QTL Cm24 Cardiac mass QTL 24 Rattus norvegicus
QTL Lanf1 Left ventricular atrial natriuretic factor QTL 1 Rattus norvegicus
Gene Nppa natriuretic peptide A Rattus norvegicus
QTL Strs2 Sensitivity to stroke QTL 2 Rattus norvegicus

Additional Information