RGD Reference Report - Expression of MARCKS effector domain mutants alters phospholipase D activity and cytoskeletal morphology of SK-N-MC neuroblastoma cells. - Rat Genome Database

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Expression of MARCKS effector domain mutants alters phospholipase D activity and cytoskeletal morphology of SK-N-MC neuroblastoma cells.

Authors: Morash, SC  Douglas, D  McMaster, CR  Cook, HW  Byers, DM 
Citation: Morash SC, etal., Neurochem Res. 2005 Nov;30(11):1353-64.
RGD ID: 9685306
Pubmed: PMID:16341931   (View Abstract at PubMed)
DOI: DOI:10.1007/s11064-005-8220-6   (Journal Full-text)

Stable overexpression of myristoylated alanine-rich C-kinase substrate (MARCKS) is known to enhance phorbol ester stimulation of phospholipase D (PLD) activity and protein kinase Calpha (PKCalpha) levels in SK-N-MC neuroblastoma cells. In contrast, expression of MARCKS mutants (S152A or S156A) lacking key PKC phosphorylation sites within the central basic effector domain (ED) had no significant effect on PLD activity or PKCalpha levels relative to vector control cells. Like control cells, those expressing wild type MARCKS were elongated and possessed longitudinally oriented stress fibers, although these cells were more prone to detach from the substratum and undergo cell death upon phorbol ester treatment. However, cells expressing MARCKS ED mutants were irregularly shaped and stress fibers were either shorter or less abundant, and cell adhesion and viability were not affected. These results suggest that intact phosphorylation sites within the MARCKS ED are required for PLD activation and influence both membrane-cytoskeletal organization and cell viability.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
activation of phospholipase D activity  IDA 9685306 RGD 
positive regulation of protein kinase C activity  IDA 9685306 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
bleb  IDA 9685306 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Marcks  (myristoylated alanine rich protein kinase C substrate)


Additional Information