RGD Reference Report - Regulation of the voltage-gated potassium channel KCNQ4 in the auditory pathway. - Rat Genome Database

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Regulation of the voltage-gated potassium channel KCNQ4 in the auditory pathway.

Authors: Chambard, JM  Ashmore, JF 
Citation: Chambard JM and Ashmore JF, Pflugers Arch. 2005 Apr;450(1):34-44. Epub 2005 Jan 20.
RGD ID: 9586011
Pubmed: PMID:15660259   (View Abstract at PubMed)
DOI: DOI:10.1007/s00424-004-1366-2   (Journal Full-text)

The potassium channel KCNQ4, expressed in the mammalian cochlea, has been associated tentatively with an outer hair cell (OHC) potassium current, I(K,n), a current distinguished by an activation curve shifted to exceptionally negative potentials. Using CHO cells as a mammalian expression system, we have examined the properties of KCNQ4 channels under different phosphorylation conditions. The expressed current showed the typical KCNQ4 voltage-dependence, with a voltage for half-maximal activation (V(1/2)) of -25 mV, and was blocked almost completely by 200 microM linopirdine. Application of 8-bromo-cAMP or the catalytic sub-unit of PKA shifted V(1/2) by approximately -10 and -20 mV, respectively. Co-expression of KCNQ4 and prestin, the OHC motor protein, altered the voltage activation by a further -15 mV. Currents recorded with less than 1 nM Ca(2+) in the pipette ran down slowly (12% over 5 min). Buffering the pipette Ca(2+) to 100 nM increased the run-down rate sevenfold. Exogenous PKA in the pipette prevented the effect of elevated [Ca(2+)](i) on run-down. Inhibition of the calcium binding proteins calmodulin or calcineurin by W-7 or cyclosporin A, respectively, also prevented the calcium-dependent rapid run-down. We suggest that KCNQ4 phosphorylation via PKA and coupling to a complex that may include prestin can lead to the negative activation and the negative resting potential found in adult OHCs.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of monoatomic ion transmembrane transport  IDA 9586011 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc26a5  (solute carrier family 26 member 5)


Additional Information